We narrowed to 605 results for: She

1. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   Blog Post

   ... is generally advised, there is little detail published on how to do it best. Scientists are typically...

2. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   Blog Post

   ...biotinylated target protein is added and after a few washes, binding is detected using a colorimetric reaction...

3. Polymerase Chain Reaction Overview and Applications

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   Blog Post

   ...     References and Resources References Shen C-H (2019) Amplification of Nucleic Acids. In: Diagnostic...

4. Hot Plasmids: Summer 2025

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   ...-58223-5.   TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search...

5. The AAVantages of AAV in CRISPR Screens

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   Blog Post

   ... Alyssa Shepard...

6. Common Injection Routes in Mice

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   Blog Post

   ... Alyssa Shepard...

7. Production of Virus in Insect Versus Mammalian Cells

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   Blog Post

   ... Alyssa Shepard...

8. Viral Vectors 101: Voltage Indicators

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   Blog Post

   ...example is attractive due to the large number of sheer molecules and signal you can generate without disturbing...

9. 3D Printing Meets CRISPR Cas9

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   Blog Post

   ...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...

10. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post

    ...fluorescently-tagged proteins at endogenous loci, shedding light on previously unknown protein functions....

11. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post

    ...strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which...

12. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ...Addgene. Publication: If the plasmid has been published, the paper in which it was first used is listed...

13. Genome-wide Screening Using CRISPR

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    Blog Post

    ...mutated. Findings from this type of experiment can shed light on the mechanism by which cells gain resistance...

14. Which Fluorescent Protein Should I Use?

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    Blog Post

    ... ilovegfp – a site with very comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent...

15. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post

    ...time, you've got a collection of reusable, off-the-shelf genetic elements that allow you to begin to imagine...

16. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post

    ... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...

17. Isolating Genomic Regions of Interest with the CRISPR System

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    ...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

18. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

    ...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

19. A Guide to Starting Your Own Journal Club

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    Blog Post

    ...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...

20. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    Blog Post

    ...specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors...