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... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing...

...work has focused on modifying the outside protein shell, or capsid, of the virus so that it only enters ...

... a project forward, transductions should not be rushed into. Before you start any transduction, you should...

...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates...

...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...

...fluorescently-tagged proteins at endogenous loci, shedding light on previously unknown protein functions....

... is generally advised, there is little detail published on how to do it best. Scientists are typically...

... Alyssa Shepard...

... Alyssa Shepard...

... Alyssa Shepard...

...biotinylated target protein is added and after a few washes, binding is detected using a colorimetric reaction...

...     References and Resources References Shen C-H (2019) Amplification of Nucleic Acids. In: Diagnostic...

...example is attractive due to the large number of sheer molecules and signal you can generate without disturbing...

...-58223-5.   TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search...

...strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which...

...Addgene. Publication: If the plasmid has been published, the paper in which it was first used is listed...

...mutated. Findings from this type of experiment can shed light on the mechanism by which cells gain resistance...

... ilovegfp – a site with very comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent...

...time, you've got a collection of reusable, off-the-shelf genetic elements that allow you to begin to imagine...

...medium such as Opti-MEM or Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear...