Addgene

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Showing: 521 - 540 of 596 results

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PITChing MMEJ as an Alternative Route for Gene Editing

Type

Blog Post

...strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which...
Anatomy of a Plasmid Page at Addgene

Type

Blog Post

...Addgene. Publication: If the plasmid has been published, the paper in which it was first used is listed...
Genome-wide Screening Using CRISPR

Type

Blog Post

...mutated. Findings from this type of experiment can shed light on the mechanism by which cells gain resistance...
Which Fluorescent Protein Should I Use?

Type

Blog Post

... ilovegfp – a site with very comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent...
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Type

Blog Post

...time, you've got a collection of reusable, off-the-shelf genetic elements that allow you to begin to imagine...
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

Type

Blog Post

... selected and ranked based on the previously established sgRNA rules and potential on- and off-target...
CRISPR Antimicrobials

Type

Blog Post

...antibiotic resistant bacteria have previously flourished. Figure 2: Two-phage strategies to resensitize...
Plasmids 101: Secondary Nanobody Toolbox

Type

Blog Post

...their N-terminal His14-bdNEDD8 tag. After several washes, this tag can be cleaved by the bdNEDP1 protease...
CRISPR 101: Ribonucleoprotein (RNP) Delivery

Type

Blog Post

...specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors...
AAVs in Retinal Gene Therapy

Type

Blog Post

...vector. For example, although researchers know that Usher Syndrome (a disease that results in combined deafness...
Isolating Genomic Regions of Interest with the CRISPR System

Type

Blog Post

...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...
An Introduction to Adenovirus

Type

Blog Post

...Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response...
Course-Based Undergraduate Research Experiences (CUREs)

Type

Blog Post

...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Type

Blog Post

...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...
A Guide to Starting Your Own Journal Club

Type

Blog Post

...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

Type

Blog Post

... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...
Tips for Screening with Yeast Two Hybrid Systems

Type

Blog Post

...with a molecule of interest (the ‘bait’) can be ‘fished out’ for further study. In Y2H screens, a group...
Cpf1: A New Tool for CRISPR Genome Editing

Type

Blog Post

...about Cpf1 and check out our CRISPR guide for a refresher on CRISPR/Cas9. Figure 1: Cpf1 is a single...
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Type

Blog Post

...structure, first reported in 1996, is an eleven β-sheet-containing “barrel” shape, with the chromophore ...
General Transfection

Type

Protocol

...medium such as Opti-MEM or Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear...

Showing: 521 - 540 of 596 results

Results per page:

PITChing MMEJ as an Alternative Route for Gene Editing

Anatomy of a Plasmid Page at Addgene

Genome-wide Screening Using CRISPR

Which Fluorescent Protein Should I Use?

Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

CRISPR Antimicrobials

Plasmids 101: Secondary Nanobody Toolbox

CRISPR 101: Ribonucleoprotein (RNP) Delivery

AAVs in Retinal Gene Therapy

Isolating Genomic Regions of Interest with the CRISPR System

An Introduction to Adenovirus

Course-Based Undergraduate Research Experiences (CUREs)

INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

A Guide to Starting Your Own Journal Club

Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

Tips for Screening with Yeast Two Hybrid Systems

Cpf1: A New Tool for CRISPR Genome Editing

Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

General Transfection

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