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... is generally advised, there is little detail published on how to do it best. Scientists are typically...

...     References and Resources References Shen C-H (2019) Amplification of Nucleic Acids. In: Diagnostic...

... Alyssa Shepard...

... Alyssa Shepard...

... Alyssa Shepard...

...example is attractive due to the large number of sheer molecules and signal you can generate without disturbing...

...-58223-5.   TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search...

...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...

...fluorescently-tagged proteins at endogenous loci, shedding light on previously unknown protein functions....

...strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which...

...Addgene. Publication: If the plasmid has been published, the paper in which it was first used is listed...

...mutated. Findings from this type of experiment can shed light on the mechanism by which cells gain resistance...

... ilovegfp – a site with very comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent...

...time, you've got a collection of reusable, off-the-shelf genetic elements that allow you to begin to imagine...

...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...

...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

...Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response...

...their N-terminal His14-bdNEDD8 tag. After several washes, this tag can be cleaved by the bdNEDP1 protease...

... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...