We narrowed to 609 results for: She

1. Mouse Modeling, Part 2: Breeding and Crossing Mice

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   Blog Post

   ...strategy that uses genetically engineered mice. Best wishes on your experiment! References Becher, Burkhard...

2. Tips for Titering Your Lentiviral Preps

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   Blog Post

   ... a project forward, transductions should not be rushed into. Before you start any transduction, you should...

3. How-to: 5 Steps to a Great Panel Discussion

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   Blog Post

   ...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates...

4. 3D Printing Meets CRISPR Cas9

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   Blog Post

   ...could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an...

5. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ...fluorescently-tagged proteins at endogenous loci, shedding light on previously unknown protein functions....

6. Viral Vectors 101: Voltage Indicators

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   ...example is attractive due to the large number of sheer molecules and signal you can generate without disturbing...

7. Hot Plasmids: Summer 2025

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   ...-58223-5.   TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search...

8. The AAVantages of AAV in CRISPR Screens

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   Blog Post

   ... Alyssa Shepard...

9. Common Injection Routes in Mice

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   Blog Post

   ... Alyssa Shepard...

10. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post

    ... Alyssa Shepard...

11. Polymerase Chain Reaction Overview and Applications

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    ...     References and Resources References Shen C-H (2019) Amplification of Nucleic Acids. In: Diagnostic...

12. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    Blog Post

    ...biotinylated target protein is added and after a few washes, binding is detected using a colorimetric reaction...

13. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    Blog Post

    ... is generally advised, there is little detail published on how to do it best. Scientists are typically...

14. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post

    ...strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which...

15. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ...Addgene. Publication: If the plasmid has been published, the paper in which it was first used is listed...

16. Genome-wide Screening Using CRISPR

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    ...mutated. Findings from this type of experiment can shed light on the mechanism by which cells gain resistance...

17. Which Fluorescent Protein Should I Use?

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    ... ilovegfp – a site with very comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent...

18. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post

    ...time, you've got a collection of reusable, off-the-shelf genetic elements that allow you to begin to imagine...

19. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    Blog Post

    ... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

20. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    Blog Post

    ...specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors...