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We narrowed to 900 results for: TRI

Showing: 521 - 540 of 900 results
  1. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...also gave us a few goodies which included an NEB drawstring backpack and a double wall insulated glass to...workshop. Future workshops for Addgene Addgene strives to be a great resource for the research community...
  2. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...This post was contributed by guest blogger Jessica Polka, a Postdoctoral Research Fellow with Pamela ...other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond these applications...
  3. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...This post was contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed...
  4. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...tissue types, recombinases allow you to further restrict gene expression to areas in which the recombinase...expression patterns, thus allowing you to further restrict gene activation to cells expressing both Cre and...
  5. How Addgene Creates Content

    Type
    Blog Post
    ...knowledge, related to the types of materials we help distribute and the research communities that we serve. ...on a case-by-case basis, as many of our blog contributors primarily write in the areas in which they have...
  6. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...These modifications result in conformationally restricted RNA which improves mismatch discrimination and... specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce the innate immune response...
  7. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...References and resources References Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson ..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...
  8. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...This is the role of Gag proteins: they contain the matrix, capsid, and nucleocapsid viral components needed...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...
  9. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...This guest post was contributed by Nathan Sanders of ComSciCon, the Communicating Science Conference ...employer that your past work has prepared you to contribute at a high level to their team.  When you're writing...
  10. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...Chromatin Cheryl Arrowsmith James Bradner Kerstin Bystricky Courtney Hodges Sharon Dent Archa Fox Elaine...theReddyLab), Thoru Pederson, Joanna Wysocka, Kerstin Bystricky, Ben Martin, and Berkley Gryder. Addgene depositors...
  11. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you... mismatch cleavage assays to detect mutations triggered by engineered nucleases. G3 (Bethesda) 5, 407–...
  12. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...This blog post was contributed by guest blogger Kate Palozola Traditional lab notebooks just won't cut... best way to find a system that does is through trial and error, and by asking for tips from others. Comment...
  13. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...focus on development, along with the inherent trickiness of using and optimizing GCE, led to a large toolbox...balance of trade-offs. This balance can be extremely tricky to find: each of the factors can negatively impact...
  14. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...we wanted to take a look back at some of the contributions from our depositors. Below are the 15 top requested...Zhang lab codon-optimized SpCas9. Using the BbsI restriction site, a pair of annealed oligos can be cloned...
  15. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...top of pre-made plates, make sure it is evenly distributed and allow sufficient drying time before use. ...candidates for more specific analysis, like PCR or restriction digest. Make sure you use a proper E. coli ...
  16. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...Last updated Sept 1, 2020. This post was contributed by David Wyatt and Dale Ramsden, UNC at Chapel Hill...mutations at off-target sites. Christina Mork contributed to the update of this post. Thank you to our ...
Showing: 521 - 540 of 900 results