Skip to main content

We narrowed to 749 results for: UTY

Showing: 521 - 540 of 749 results
  1. Reflections and Looking Ahead

    Type
    Blog Post
    ...thoroughly characterized antibodies, and our contributions to key research papers are already inspiring...
  2. Editor's Choice, August 2016

    Type
    Blog Post
    ...how to give a great science talk from Addgene Executive Director Joanne Kamens. I highly recommend taking...
  3. AAV ddPCR Titration

    Type
    Protocol
    ...in the dilution BSC. Prepare 1X dilution buffer (see recipe above). Save ~25 µL of 1X dilution buffer ... carefully add the dilution buffer to the dilution plate according to the dilution scheme listed in step...Pro-Tip Prepare the viral dilutions in a separate biological safety cabinet (dilution BSC) than that used for...touching the screen. Prepare the Serial Dilution Place a 48-well dilution plate in a chilled 96-well freezer... add 5 µL of each viral sample to Dilution 1 in the 48-well dilution plate and pipette 5–10 times to mix...change tips each time. Dilution 1 (20X): 5 µL in 95 µL 1X PCR buffer (1:20) Dilution 2 (20X): 5 µL in 95 ...PCR buffer (1:400) Dilution 3 (20X): 5 µL in 95 µL 1X PCR buffer (1:8,000) Dilution 4 (20X): 5 µL in 95...
Showing: 521 - 540 of 749 results