We narrowed to 749 results for: UTY
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TypeBlog Post... will get busy with GreenGate, producing and contributing more modules into the public domain in the process...
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The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post...individual plasmids to 5,000 different research institutions. This has given us a unique window into technology... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog Post...We began distributing ready-to-use virus preps through our viral service in late 2016 and requests are... -
Reflections and Looking Ahead
TypeBlog Post...thoroughly characterized antibodies, and our contributions to key research papers are already inspiring... -
Editor's Choice, August 2016
TypeBlog Post...how to give a great science talk from Addgene Executive Director Joanne Kamens. I highly recommend taking... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post...representing core nodes that, when over-expressed, constitutively activated or inactivated the pathway. “The ... -
Management for Scientists: Seeking Feedback
TypeBlog Post...and listens in roughly equal measure, keeping contribution short and sweet. One key to this is ensuring... -
From our Table to Yours: An Inside Look at Lunch at Addgene
TypeBlog Post...naturally divided into smaller functional teams. Our Executive Director, Joanne Kamens, responded by starting... -
A new platform for fast functional characterization of potentially cancerous mutations
TypeBlog Post...is important for the development of targeted therapeutic interventions for cancer. This screening platform... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog Post... PI is responsible for determining meaningful solutions. PI-trainee communication builds a positive lab... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog Post..., vendors, Addgene supporters, and of course, Executive Director, Joanne Kamens, and founders, Melina ... -
Tag Your Favorite Yeast Genes with Ease
TypeBlog Post...partial gene deletion, overexpression (by promoter substitution), and tagging at either the N- or C-terminus... -
AAV ddPCR Titration
TypeProtocol...in the dilution BSC. Prepare 1X dilution buffer (see recipe above). Save ~25 µL of 1X dilution buffer ... carefully add the dilution buffer to the dilution plate according to the dilution scheme listed in step...Pro-Tip Prepare the viral dilutions in a separate biological safety cabinet (dilution BSC) than that used for...touching the screen. Prepare the Serial Dilution Place a 48-well dilution plate in a chilled 96-well freezer... add 5 µL of each viral sample to Dilution 1 in the 48-well dilution plate and pipette 5–10 times to mix...change tips each time. Dilution 1 (20X): 5 µL in 95 µL 1X PCR buffer (1:20) Dilution 2 (20X): 5 µL in 95 ...PCR buffer (1:400) Dilution 3 (20X): 5 µL in 95 µL 1X PCR buffer (1:8,000) Dilution 4 (20X): 5 µL in 95... -
Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry
TypeBlog Post...the COVID-19 plasmids first became available: distribution was limited to academic scientists. Expanding... -
IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging
TypeBlog Post...staining and chemical bleaching to allow for high-resolution imaging without degrading the tissue (Radtke ... -
2023 and 2024: Reflections and Looking Ahead
TypeBlog Post...greater value for the scientific community. As the executive director of Addgene, it is my privilege to uphold... -
What's New in CRISPR - March 2020
TypeBlog Post...Caulobacter crescentus Figure 1: Serial dilutions of CRISPRi in Caulobacter cells for ctrA gene ... -
A Better Way to Get Customer Support: The Help Center
TypeBlog Post...speak to a human being, you may not even get a solution to the problem you called about. Next, you try... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog Post...seeking a first job after the academic bench. Utilizing existing relationships as part of the job application... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...loxP sites in mammalian genomes: genome-wide distribution and relevance for the efficiency of BAC/PAC ...