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Addgene

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We narrowed to 743 results for: UTY;

Showing: 521 - 540 of 743 results

  8. AAV ddPCR Titration

    Type
    Protocol
    ...in the dilution BSC. Prepare 1X dilution buffer (see recipe above). Save ~25 µL of 1X dilution buffer ... carefully add the dilution buffer to the dilution plate according to the dilution scheme listed in step...Pro-Tip Prepare the viral dilutions in a separate biological safety cabinet (dilution BSC) than that used for...touching the screen. Prepare the Serial Dilution Place a 48-well dilution plate in a chilled 96-well freezer... add 5 µL of each viral sample to Dilution 1 in the 48-well dilution plate and pipette 5–10 times to mix...change tips each time. Dilution 1 (20X): 5 µL in 95 µL 1X PCR buffer (1:20) Dilution 2 (20X): 5 µL in 95 ...PCR buffer (1:400) Dilution 3 (20X): 5 µL in 95 µL 1X PCR buffer (1:8,000) Dilution 4 (20X): 5 µL in 95...

  17. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...of 2 x 10 8 dilution 90 2 x 10 7 10 of 2 x 10 7 dilution 90 2 x 10 6 10 of 2 x 10 6 dilution 90 2 x 10 ...Total dilution Dilution 1 (DNase step) 5 uL AAV stock 45 uL 10X 10X Dilution 2 5 uL Dil. 1 95 uL 20X 200X...200X Dilution 3 20 uL Dil. 2 80 uL 5X 1000X Dilution 4 20 uL Dil. 3 80 uL 5X 5000X Dilution 5 20 uL Dil...25000X Dilution 6 20 uL Dil. 5 80 uL 5X 125000X Dilution 7 20 uL Dil. 6 80 uL 5X 625000X Dilution 8 20 ... To obtain a solution at 2 x 10 9 molecules/μL: 1.59 x 10 11 / 2 x 10 9 = 79.8X dilution ...10 5 10 of 2 x 10 5 dilution 90 2 x 10 4 10 of 2 x 10 4 dilution 90 2 x 10 3 Pro-Tip To help stabilize ... standard dilution. Dilute DNase-treated and AAV reference samples according to the dilution scheme in...

  18. Lentivirus ddPCR Titration

    Type
    Protocol
    ...transfer 200 µL of the first dilution into the next dilution. Repeat for all dilutions as indicated in the chart... virus dilutions were used to transduce cells: Initial dilution factor of virus stock Dilution factor ... µL 5 200 µL of 2.5-fold dilution 200 µL 400 µL 10 200 µL of 5-fold dilution 200 µL 400 µL 20 200 µL of... of 10-fold dilution 200 µL 400 µL 40 200 µL of 20-fold dilution 200 µL 400 µL 80 200 µL of 40-fold dilution... 160 200 µL of 80-fold dilution 200 µL 400 µL Add 150 µL of each viral dilution to a well of a 6-well ... viral dilution). Remember, this is an additional 10-fold dilution of the virus stock! Don’t forget to...~2 as you progress from the higher dilutions to the lower dilutions (blue). As shown in the image (Figure...
Showing: 521 - 540 of 743 results