We narrowed to 770 results for: VARS
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TypeBlog Post...introducing foreign DNA into the genome is useful for a variety of applications. For example, specific tissues ...transparency of the embryo—have proven useful in a variety of research applications. Lineage tracing studies...
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CRISPR-mediated Plant Base Editors
TypeBlog Post...target genomic locus/loci, choose a base editor variant from Table 1 depending on the PAM sequence. Avoid...Expanding the base editing scope in rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID... -
Transferable Skills Guide: Career Planning Resources
TypeBlog Post...researchers feel that they lack formal training in a variety of transferable skills. At Addgene we've set out...indeed.com or monster.com, where you’ll find a variety of listings. Post your CV and make it visible, ... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog Post...18px;font-family: helvetica;","color":"#FFFFFF","variant":null,"font":"helvetica","style":"font-family: ..., G., Johansen, N. J., Hooper, M., Omstead, V., Vargas, S., Lerma, M. N., Taskin, N., Weed, N., Laird,... -
Using Video to Share Your Science: We Share Science
TypeBlog Post... able to quickly synthesize information from a variety of fields in order to provide useful solutions ...been a long brewing frustration for me. I read a variety of psychology, business, personnel, and sociology... -
5 Reasons to Use Reddit for Science Communication
TypeBlog Post... participate in forum discussions about a wide variety of topics. Individual forums are hosted on subreddits...sometimes, civil. r/Science in particular hosts a variety of conversations with people who work in many different... -
CRISPR Activation: A Practical Guide
TypeBlog Post... Cas9 activator should I use? There are a wide variety of activators you can use for your experiments....Addgene Blog Learn how to Choose the Best Cas9 Variant for Your Next Experiment Find out More about Genome-wide... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post...expression? Low expression outcomes can result from variety of sources, so fear not—there are a few simple ...up a small-scale expression experiment to test variables such as temperature, time, and media conditions... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...expression with an overlapping AAV since both of these variables influence whether cells will be co-transduced ...donor template. Drawbacks: Transgene expression varies with this approach, ranging from ~10% expression... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...homology arm, but keep in mind these numbers may vary based on your locus of interest and experimental...ssODNs in mouse models. Although HDR efficiency varies across loci and experimental systems, ssODN templates... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog Post...genome editing. In this post we'll walk through a variety of ways you can use AAVs to improve your genome...being the vector of choice for gene delivery to a variety of cell types, now you know that AAVs can provide... -
An Integrin Antibody Toolkit from IPI
TypeBlog Post...are chimeric antibodies composed of rat or mouse variable regions fused with rabbit constant domains. All...s work with the Boston Children’s Hospital and Harvard-based lab of Tim Springer, the founder of IPI. ... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog Post...to the intended edit rather than some passenger variant that came along with the single cell clone (although...SaCas9, NmeCas9, Cas12a enzymes, and engineered variants thereof offers additional PAM options that can... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...demethylation and induce transcription at CpG regions of various promoters. Other researchers additionally fused...applications direct catalytically dead dCas9 fused to a variety of epigenetic modifiers to specific loci without... -
Viral Vectors 101: Optogenetic Tools
TypeBlog Post...responsive to light in combination with retinal) variations designed to give researchers flexibility in using...photoswitches can be used in a single pathway to activate various protein regulators at different points in the pathway... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...and red fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns including...PMC4942401. 5. Susuki, K., et al. (2016) Five colour variants of bright luminescent protein for real-time multicolour... -
Finding Your Science Policy Path
TypeBlog Post... worked for seven different organizations in a variety of science policy roles including fellowships, ...folks who work in science policy come from a wide variety of backgrounds with technical expertise that ranges... -
A History of Genome Engineering in Popular Culture
TypeBlog Post...curing diseases caused due to single nucleotide variations in the genome. Due to the rise of CRISPR in...engineering and open advertisement of the technology on various non-scientific platforms, CRISPR has also transcended... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post...investigator (PI) Runs the lab Writes a lot of grants Varying degrees of engagement with what happens in the ...Undergraduate student Roles and responsibilities can vary "Lab assistants" usually wash glassware, prep media... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...the software piñata has been filled with slight variations on a theme without many resources to review them...available CRISPR software tools, what problems various tools are trying to solve, and finally how we might...