We narrowed to 835 results for: ang

... Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these three luminescent...eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al. also envision...

... is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014...PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries for...

... of blue light, iLID undergoes a conformational change allowing high affinity binding to SspB, brings ...host organism, the expression of some genes can change depending on the location. Current methods for ...

...Morning. Karen was a founding member of Mass Medical Angels, a venture mentor at MIT and served on the Boards... Jennifer Tsang...

... Sons of Sevenless (SOS), a guanine nucleotide exchange factor that activates Ras. They expressed this...message reaches the nucleus where gene expression changes lead to cell proliferation and differentiation....

...our first blog post on choosing a Cas9, the Feng Zhang Lab had described eSpCas9, while the Keith Joung...potentially treatments. Cas12b, characterized by the Zhang (Strecker et al., 2019) and Li (Teng et al., 2018...

...Developmental Editor at F1000Research.  With many changes taking place in the world of science publishing...proofread your manuscript and help you correct any language- and spelling mistakes. A manuscript that is well...

... mentor met with a group of mentees to get more “bang for the buck”. Listen to our podcast interview with...years. They helped one another navigate career changes and overcome barriers by sharing broader perspectives...

...genome editing tool with which one can introduce changes into the genomes of mammalian or other cells. TALENs...genetic backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare the TALEN ...

...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...harder it is to throw in last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means...

...and several contaminating proteins (blue, green, orange) flows through the Ni2+-containing resin. The protein...polyhistidine containing contaminating protein (orange). A low imidazole wash buffer disrupts the weak...

... hybrid molecules both exist and have a diverse range of functions within mammalian cells. Here, we will... much more! Resources and References References Wang, I. X., Grunseich, C., Fox, J., et al., Human proteins...

...parts in the micromolar or nanomolar concentration range. Reactions to happen under lots of conditions ...from Promega, including many HaloTag plasmids Exchangeable HaloTag Ligands HaloTag Reversible binding of...

....  Next, Fab and Fab’ fragments are almost interchangeable, but the extra bit of heavy chain on a Fab’...:703–706. https://doi.org/10.1177/34.6.3084626  Zhang Q, Miyamoto A, Watanabe S, Arimori T, Sakai M, Tomisaki...

...otherwise it would be a lot quicker to pick up new languages!) Jargon has a place in science communication,...student groups and being aware of cognitive load changed the way I thought about engaging with learners....

...protocol modifications, and ultimately decided on the changes to implement. As with any skill, there will always...solving skills and learn to apply them flexibly in a range of contexts. As such, scientists are uniquely prepared...

...monkeys. Surprisingly, we found that this small change made a big difference. Replacing the mCherry with...understanding of the physiology and pathology of the basal ganglia in movement disorders.   References and Resources...

...abilities is that its fluorescence emission spectra changes when it goes from a more neutral pH compartment...emission spectra. Component Excitation Emission pH Range DsRed.T3 488, 543*, 568 nm 587 nm ~4.9 - ...

...to Sonali Roy, Magdalena Julkowska, and Jennifer Tsang for editing. Additional resources on the Addgene... Angela Abitua...

...al., 2014; Ma et al., 2015; Shalem et al., 2014; Wang et al., 2014), knock down (Gilbert et al., 2014)...: 24336571. PubMed Central PMCID: PMC4089965. 9. Wang, Tim, et al. "Genetic screens in human cells using...