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We narrowed to 951 results for: cha

Showing: 521 - 540 of 951 results

  1. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ... loci of integration [8].   Figure 1: Mechanism of the piggyBac™  transposase/transposon gene ...CRISPR/Cas9, TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely...nuclease technology with the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon...researchers to avoid making unnecessary base pair changes as would be the case with ssODNs. One minor drawback...

  2. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... called SNAPf. eDHFR: The Virginia Cornish and Michael Sheetz labs demonstrated in 2005 that proteins ...and see the results right away. Or perform pulse-chase experiments by sequentially adding different ligands...All this flexibility comes at a price, though. Purchasing lots and lots of organic dyes or specialized ...Johnsson, K. (2021). Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7...

  3. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... genome editing system you use, there remains a chance that the observed phenotype was caused by an off-target...25566793. PubMed Central PMCID: PMC4349094. 3. Bell, Charles C., et al. "A high-throughput screening strategy...: 237-243. PubMed PMID: 25664545. 10. Gabriel, Richard, et al. "An unbiased genome-wide analysis of zinc-finger...Therapy (2015). PubMed PMID: 26502778. 13. Frock, Richard L., et al. "Genome-wide detection of DNA double-stranded...

  4. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...in a screen, to ensure that each gRNA has a good chance to contribute to the screen. In the end, this results...that code for surface proteins. One of the first challenges with a custom screen approach is to identify ...Wu (4), Yusa (5), Wang/Lander/Sabatini (6), and Chari/Mali/Church (7). These reference libraries contain... 28162770. PubMed Central PMCID: PMC5445660. 7. Chari, Raj, et al. "Unraveling CRISPR-Cas9 genome engineering...

  5. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    ... research papers, blogs, free courses, YouTube channels, books, podcasts, and communities to help people...Niko McCarty; adapted from Bruce Wetzel and Harry Schaefer, NCI, NIH | License   I’m updating it regularly...understand the contours of each topic. The video channels are also underrated and I recommend checking them...

  6. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...description of the applicable research project when purchasing export-controlled materials. Addgene will then...our normal process.   Due to recent regulation changes, an export control license for rabies materials...processing fee will be removed before your account is charged.  Timelines  We have offered VSV and SARS-COV-1...

  7. Deck the Lab 2022!

    Type
    Blog Post
    ... the 2022 #DeckTheLab contest!  and it's..... Michael Früh submission, which we have entitled "Do You...friend anymore :C pic.twitter.com/rExV3b8Q8u— Michael Früh (@Michelderapfel) December 19, 2022   Congratulations...used tip boxes for a lab supply tree and set a challenge to my lab mates. They did not disappoint. #deckthelab...

  8. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    ...Number of requests per reagents (as a horizontal bar chart) Number of requests per country (as dot plot) ...) Number of requests per year (as a bar chart) A written summary of the data, including total requests...microscopy (University of Amsterdam). He develops, characterizes, and uses genetically encoded fluorescent probes...

  9. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...Host-parasite Interactions, Institut Pasteur, Paris) that changed genome editing in the malaria community for the...it all settles down and the value of each item changes from what the reviewers so painstakingly assessed...space. The CRISPR research community hasn't had a chance to develop a consensus on the best CRISPR software... up with it. The peer review process has been challenged with many closely spaced CRISPR software submissions...

  11. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...created tagged versions of the cyclin Cdc28 and the chaperone Ydj1, both of which display sophisticated interactions...with multiple other proteins. Again, little to no change in cell morphology was observed with IT5 and IT6...methods (2015). PubMed PMID:26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and fluorescent-protein...

  13. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...biologists have spent a lot of time making things glow. Chances are you’ve used a GFP derivative to conduct subcellular...optogenetics. Even though GFP has been so thoroughly characterized, it turns out this protein has a few more secrets... LM, Wang Y, Condeelis JS, Verkhusha VV. A photoswitchable orange-to-far-red fluorescent protein, PSmOrange...

  14. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ... inferred based on correlative biochemical characteristics and ectopic reporter assays. We have been interested..., identifying these deletions, and finally characterization. Read and download the full protocol from ...Dass A, Yien YY, Chung J, Masuda T, et al. Characterization of genomic deletion efficiency mediated by...

  15. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ... desserts. We enjoyed a video highlighting the changes at Addgene over the last 10 years as well as the...fruition. The concept, though relatively simple, changed the course of scientific research. What was once... ghost writers, of course! Amy Uddin and Emma Gottschall, two of Addgene's lab technicians, gave Blugene...

  16. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    ...Navigating a fulfilling career in science can be challenging – is advice and guidance from only 2 people enough...with the researchers who joined a recent Twitter chat for Early Career Researchers.  Many of the participants...lighthearted, the article does highlight key characteristics of a good mentor: Yoda image courtesy...

  17. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...                      University of North Carolina at Chapel Hill University of Illinois Chicago                     ...beyond academia. For example: Plasmids from the Michael J. Fox Foundation are used in Parkinson’s research...carriers (SLCs) for medical research Recently, the Michael Davidson Fluorescent Protein collection, a ‘One-Stop...

  18. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    ...development, learning, and behavior poses many challenges in neuroscience with many brain regions, neuronal...specific neuronal subtypes and determine how these changes affect the phenotype. Viral vectors and genetic...specific cell type. Find some transduction characteristics of select AAV serotypes in Table 2 from the...

  19. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...homologous recombination in bacteria. These quirks change the way CRISPR-mediated genome engineering functions...While the activation of transcription is a more challenging problem than repression, scientists have deposited...the target sequence which could be adjusted by changing the sgRNA length. The system could be further ...Streptomyces, yet genome engineering remains challenging for most organisms in the genus. Work from the...

  20. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...Sometimes DNA sequences need to be “cleaned up” or changed in some way before you can analyze them. These ...complement sequence.  Filter DNA tool: Removes non-DNA characters, such as numbers and blank spaces, from the text... primer dimer estimation, and other primer characteristics. NEB Data-optimized Assembly Design (DAD): ...
Showing: 521 - 540 of 951 results