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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 911 results for: ire;

Showing: 521 - 540 of 911 results

  1. CRISPR in the Clinic

    Type
    Blog Post
    ...Systemic therapies involve CRISPR-Cas delivery directly to the patient. Patient-derived cells can alternatively...the goal of ‘fixing’ mutations or introducing a desired edit, but this particular class of treatments has...inflammatory diseases, blindness, and diabetes. Directions and obstacles for future CRISPR therapies CRISPR...

  2. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...longer incubation times and didn’t necessarily require cycling. The Pros and Cons Researchers used the...select for correctly assembled phage genomes, it required a very stringent selection process and benefited...selection, failed under normal cycling protocols, and required a 48-hour incubation at 37 °C. While the 52-piece...

  3. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Targeting Receptor Expression Using chemogenetics requires successfully introducing receptors to target cells... to control ion flux. The system allows you to directly manipulate ion channels. When working with PSAM...Magnus C, Lee P, Bonaventura J, Zemla R, Gomez J, Ramirez M, Hu X, Galvan A, Basu J, Michaelides M, Sternson...

  4. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...simple, and engineering complex cellular behaviors requires making complex manipulations to cells. For example...number in the Level 2 assembly. These pathways can require a lot of optimization - which is why MoClo is so...of a combination of multiple genes to achieve a desired effect. This is commonly used in plant biology ...

  5. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...active sites. In addition, Casɸ has a minimal PAM requirement. For instance, for Casɸ-2 the PAM sequence is... is expected to contribute to experiments that require high photostability, such as time-lapse imaging...Leopold Parts. This library delivers 2 randomly paired guides per construct and allows a reduced scale...

  6. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...that the orientation of the flanking loxP sites directs the Cre recombinase to invert, translocate, or ... the antisense orientation (Figure 1, top). The entire DNA cassette would be flanked by two pairs of target... Wendling O, Chambon P, and Ghyselinck NB. A directional strategy for monitoring Cre-mediated recombination...

  7. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...world. With three Nobel Prize winners, one NIH Director and a co-founder of BGI-China presenting, it’s... discoveries and moving beyond DNA Eric Green, Director of the National Human Genome Research Institute...proteins). The chemistry is complex and this work requires an interdisciplinary team.  Day two of the conference...

  9. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...handle or direct with a degree of skill, To work upon or try to alter for a purpose and To direct the professional... these tasks before I was a manager! Second, my direct report was not engaged because I only left him/...

  10. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...single 96-well plate for HDR that satisfy all requirements. To alleviate this bottleneck, we began building... your own purposes, or delete the originals if desired. It works as a whole or in part. For example, you... were optimized for HDR, except for TagIn. HDR requires the additional step of mutating guide sequences...

  12. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...syndrome coronavirus 2 (SARS-CoV-2), the virus may acquire mutations to escape these therapeutics. Jesse Bloom...the antibodies were able to immunoprecipitate the entire complex in three different cell lines. The Gräslund...enzyme. Compared with previous research, which required protein compartments to co-localize multiple enzymes...

  13. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...deposited their plasmid with Addgene, hoping to inspire others to explore the phenomenon, improve the tool...Evolution of a new variant Maria and the team set up a directed evolution and screening experiment with three ...topics. He embraces open science, self-publishing directly on his website (and often posting updates on social...

  14. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...This will make it difficult to properly gate your desired cell population. By compensating between fluorescent...other corners of the dot plot, compensation is not required.  In Figure 3, we also know that on most cytometers... cytometers, FITC and APC should not require compensation between each other. We can therefore conclude...

  15. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ... minor pre-recombined population that does not require the recombinase for transgene expression. Once ... of quality control is recommended but not yet required for clinical-grade materials, and is not commonly...https://doi.org/10.1089/hum.2019.277 Lecomte E, Tournaire B, Cogné B, Dupont J-B, Lindenbaum P, Martin-Fontaine...

  16. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...of antibiotic-resistant cells; during the time required to grease, place and fill a dozen cloning rings...endocrine cell lines we most commonly work with, require extra serum to survive at low densities - adding...growing cells such as HEK and CHO do not seem to require this, while nearly all endocrine cells do. While...

  17. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...level. The heartbeat – a muscle contraction – is directly related to the electrical impulse of the cardiac... to measure membrane potential and can also be directed to specific types of cells, such as neuron subtypes...For in vivo imaging applications, the ability to direct your voltage sensor selectively is especially essential...

  18. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...alternative approach to NHEJ is to utilize the Homology Directed Repair (HDR) to knock in corrective genes. For...to repair the double strand break via homology directed repair and you would probably need to use a two...human cells being subjected to scarless homology-directed genome editing (Maggio et al., 2014). Gonçalves...

  20. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...suppressors and upregulation of proto-oncogenes ­­­can directly inhibit the ability of a cell to undergo programmed...that a number of novel small molecules can bind directly, albeit weakly, to K-Ras and inhibit its activation...A single mouse click on a gene of interest will direct the investigator to a list of all available Addgene...
Showing: 521 - 540 of 911 results