We narrowed to 997 results for: lars

...If you work in a lab that regularly does immunoimaging, there’s likely a large collection of antibodies...specific to denatured versions, or even to just that particular assay.      Fig. 1: A native protein ...that has been validated for an assay that uses similar sample preparation - for instance, if you cannot...

...their name? In 1952, Joshua Lederberg set out to clarify the classification of these cytoplasmic inheritance...began to study other extrachromosomal particles, particularly Resistance or R-factors. Like F-factors, R-factors...especially lambda, was the tool of choice for molecular biologists wanting to study bacterial genetics...pSC101 became the first plasmid cloning vector and molecular biology was never the same. Over the next few ...

...meetings or calls regularly and don’t cancel, so that you drive progress with regular accountability checks...Mentoring for Scientists Guide.  You can use it to clarify and discuss values. The activity is just the start...Relevant, and Time-bound. It is a good idea to take larger goals (e.g. Figure out what I want to do after ...

...outlier, this story had us wondering: is there a larger trend here? Do papers associated with Addgene deposits...deposits are more highly cited, we went to one of the largest bibliographic databases available, Web of Science...Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology, and Biochemical Research Methods. We ...at the MIT Sloan School of Management. She is particularly interested in exploring how innovative tools...

...often have to choose the best serotype for their particular study, which includes comparing the transduction...samples are available in various serotypes of two popular control AAV encoding GFP. Check out our inventory...variant provides improved detection for tracking large populations of neurons in wide-field imaging (Dana...on availability date or to be notified when a particular catalog item is available for order please email...

...are responsible for the degradation of over 600 cellular components ultimately leading to cell fragmentation... scientists need a protease reporter that has a large dynamic range and high brightness. The Shu Lab from... pattern was consistent with TUNEL staining, a popular method to observe apoptotic cells. Lastly, the ...FlipGFP is a novel fluorogenic reporter that has a large dynamic range and high brightness. This reporter...

...drastically alter your strategy. I realized that similar types of thinking go into developing gene therapy...PLASMID is a game where you need to apply basic molecular biology, cell biology, microbiology, genetic engineering... He is also founder and consultant at FRESCI particularly interested in advanced in vitro models technology...Resource Center Resources on Addgene.org Find molecular biology educational resources Find protocol videos...

...FPs are tremendously complicated entities with a large range of characteristics (color, brightness, photostability... a project written in Python & Django, using a popular database management system (PostgreSQL), that aims...use case for FPbase is finding information on a particular FP; this can be done directly using the search...your help!), I believe this represents one of the largest available online collections of FP spectra. Every...

...the cell, and potentially spread out over an even larger area, thus necessitating even higher retrograde...methods to improve AAV production.     References 1.Clark, K. Reed. "Recent advances in recombinant adeno-...Adeno-associated Virus Transduction and Integration. Molecular therapy : the journal of the American Society ...anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways for defense ...

...levels for 6 days - after just 15 minutes of FUS.  Similar constructs to the one above can be made for expressing...But why might you need a second GFP antibody?  Similarities:  Generated against full length GFP and converted...the group rationally designed GFP variants with similar capabilities. If you’re looking for a super stable...hfYFP retains folding and fluorescence in high molarity GdnHCl, at high temperatures, and in the presence...

...orchestrated movements of networks of proteins consuming molecular fuel sources. While the importance of understanding... realized that these mutations clustered in a particular region of the R-body. Further mutatgenesis of...penetrate other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond...

...enable the assembly of larger systems. The work of Schmidl et al. also represents a large step forward in optimizing...required per system. Applications of these systems The large dynamic range of these systems ensures that they...TCSs have been identified in bacteria, and with similar optimization methods, many of these systems could...

...contain all fluorescent proteins in common use (particularly not the most recently published ones), they ...if you know that your protein of interest has a similar spectrum. For example, here's a screenshot from...TagRFP excitation and the emission filter collects a larger fraction of the TagRFP emission than the mCherry...Fluorescein / Rhodamine / Cy5 (e.g. Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / ...

...of Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription and translation...develop and test hypotheses. Students then use cellular extracts as a means to rapidly prototype DNA circuits...biobuilder.org) or by emailing [email protected]. Of particular interest this fall:  The BioBuilderClub BioBuilderClub...for all who wish to participate. All new teams, large or small, receive the BioBuilder textbook, a laboratory...

...with a transfectable cell line. 2) They require larger expression vectors which are often more difficult...into viral vectors. Cas9 multiplexing vectors are larger because they require regulatory sequences to allow...individual promoters).  spCas9 and its gRNAs are also larger than their Cpf1 counterparts. Table 2. Cas9 Multiplexing...oligos that consist of direct repeats and crRNA.  Similar to a jigsaw puzzle, the oligos were designed with...

...preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing plasmids...dependent on grants. Aiming to operate under a similar model for our Viral Service, we solicited seed ...support we are able to provide faster access to a larger number of viral preps. Visit our Sponsors and Supporters...scientists who have gone the extra mile for this particular project. John Doench and David Root from the ...

...Elasticsearch to support the different usage patterns the larger team identified. The basic function of a search...scientific domain in general and of our data in particular and our deep understanding of Addgene’s customer...categorization of our information in order to more granularly customize the search engine, and a scoping mechanism...depositors (see below). Even if the user experience is similar, the way the software searches through our catalog...

.../phototoxicity Not susceptible Susceptible Subcellular imaging Improving Well-established High-throughput...bioluminescent reporter to be cloned and remains popular, other luciferases have been identified in the ...luciferase is measured via the light produced. A similar approach can be used to analyze cell signaling ...brings the two luciferase components together. A similar approach can test protein-protein interactions ...

...back” overhangs, they can be annealed, forming a circular product with four nicks easily repairable by the...terminators. Another potential issue is sequence similarity. If fragments in a multicomponent assembly have...its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless, unlike...Protocol Gibson Assembly Blog Post Choosing a Molecular Cloning Technique Choosing Your Perfect Plasmid...

...in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated viruses...subject delivery, as immunogenicity was previously a large barrier for gene therapy. In fact, AAV gene therapies...multiple diseases, including hemophilia B and spinal muscular atrophy.   Type of Virus Pros Cons Retrovirus...High Gene Expression Infects Non-Dividing Cells Large Packaging Capacity (>8kb) High Transduction Efficiency...