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Showing: 521 - 540 of 550 results
  1. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:1143157. doi: 10.3389/fbioe.2023.1143157...
  2. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...living cells and organisms with TurboID. Nature Biotechnology, 36(9), 880–887. https://doi.org/10.1038/nbt...
  3. CRISPR Guide

    Type
    Guide
    ...egularly I nterspaced S hort P alindromic R epeat) technologies. Before CRISPR, genome engineering approaches...CRISPR one of the most scalable genome editing technologies, making CRISPR perfect for genome-wide screens...population, you will look for relevant hits using NGS technology. For more detail on using CRISPR libraries for...identified by in vivo screening in yeast. Nature Biotechnology , 36 (3), 265–271. PMID: 29431739 Chen, J. S...CRISPR-Cas nucleases in human cells. Nature Biotechnology , 31 (9), 822–826. PMID: 23792628 Fu, Y., Sander...specificity using truncated guide RNAs. Nature Biotechnology , 32 (3), 279–284. PMID: 24463574 Gao, S., Wang...specificity of RNA-guided Cas9 nucleases. Nature Biotechnology , 31 (9), 827–832. PMID: 23873081 Hu, J. H.,...
  4. Molecular Biology Reference

    Type
    Guide
    ...synthetic amino acids. Plasmids and Recombinant DNA Technology Chemistry techniques enable the isolation and...specific DNA sequence. The field of recombinant DNA technology has overcome this obstacle by enabling the preparation...purification) and manipulation. With current cloning technology, it is easy to create and modify plasmids containing...editing is most commonly accomplished using CRISPR technology. CRISPR is composed of a DNA endonuclease and...plasmids used in the field of recombinant DNA technology have been optimized for studying and manipulating...thi-1 gyrA96 relA1 tonA EPI300 LGC Biosearch Technologies; MilliporeSigma F- mcrA Δ(mrr-hsdRMS-mcrBC) ...100 µg/mL Chloramphenicol 25 mg/mL (dissolve in Ethanol) 25 µg/mL Hygromycin B 200 mg/mL 200 µg/mL Kanamycin...
  5. Plan Your Experiment

    Type
    Guide
    ...more information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different... for CRISPR-Based genome editing. Trends in Biotechnology , 36 (2), 173–185. https://doi.org/10.1016/j.tibtech... analysis of CRISPR–Cas experiments. Nature Biotechnology , 38 (7), 813–823. https://doi.org/10.1038/s41587...
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