...New to the bench? Fragmid depositor John Doench reports even the greenest members of his lab, high school...modular cloning approach, it’s easy to switch out reporters, Cas proteins, guide cassettes, promoters, N’ ...vectors are available? What you’re building is important — but so is where you’re building it! Table 1 ...

...that infect bacteria), many of which are doing important work in our microbiomes. Given that viruses are... and the type of virus, there have been varied reports of titer losses from 5% to 50% per freeze thaw ...vector: optimization of conditions for storage, transport and delivery." Japanese journal of cancer research...

...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...efficiency across CRISPR nucleases and NgAgo is an important next step for the field. Based on what we know...

...that the body’s architecture and plumbing are important. We started with learning the head is connected...and the G protein (supplied in trans) will be incorporated into the viral envelope (but not the viral genome...B. Retrograde monosynaptic tracing reveals the temporal evolution of inputs onto new neurons in the adult...

...Professor at Boston College. National reports stress the importance of providing authentic research experiences... is particularly interested in strategies to incorporate research into undergraduate courses and in communicating...

...still working at the bench. It was a fantastic opportunity to talk to so many scientists who were potential...field. Nobel Laureates and the ever expanding importance of RNA The second keynote lecture was presented... labs are using CRISPR/cas9 and more are being reported. Zhou Songyang from Baylor College of Medicine...

...experiments where maintaining library diversity is important, while lower transfer rates are only suited for...measured by knockdown of a constitutely expressed RFP reporter, was variable across bacterial strains, ranging...Klebsiella pneumoniae, and P. aeruginosa (all opportunistic pathogens) were grown in presence of trimethoprim...

...all the components of the biosensor in optimal proportions from a vector backbone that allows lentiviral...Waldron We recently added two antibodies against important targets for neurodegeneration research: Anti-MAPT...6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...

...CRISPR screens rely on lentiviral systems, a fact supported by the wide array of lentiviral-based CRISPR libraries...locations that could cause deleterious effects. It is important to note, however, that while AAV rarely integrates..., CRISPR may increase this rate. Specifically, portions of the AAV genome can integrate at double-stranded...

.... Andrew and Maria were encouraged by previous reports that described magnetic responses of cryptochrome...reproduced their EGFP findings since the initial report in 2023.)     Figure 1: Fluorescence intensity...frequent collaborator and is happy to give advice and support for follow-up studies. “We don’t have a vision ...

...combs. To make genetic crosses properly, it is important to isolate females from males while they are still... after hatching into their adult phase. It is important to clear out the parent crosses from the container...feature when setting up genetic crosses. More importantly, this knowledge will stop you from embarking ...

...Scientific Reports paper introducing the delivery method in 2014, Manuel Goncalves lab report that AdV-...the likelihood that donor DNA will interact with sporadic double-stranded chromosomal DNA breaks “that always...

...post was updated on Aug 20, 2020. What genes are important in your phenotype of interest? Many scientists...not entirely known. Identifying which genes are important for a phenotype can lead to a wealth of additional...strong evidence that a particular gene is actually important for a given phenotype. Each hit from the screen...

... These parts have been carefully engineered to support reliable functional composition. In other words...YouTube primer on BIL gates.) Addgene: Why is it so important to you that these parts be made freely available...language is put into practice? Endy: I think it's important to understand that these platforms, or pieces ...

...conditions when compared to FRET. However, it’s important to note that BiFC is irreversible — FP fragments...interact or not? — FRET is preferable when the temporal dynamics of PPIs are the focus of study. Many...equal parts neuroscientist, writer, and extreme sports enthusiast. He recently obtained his Ph.D. in neuroscience...

...dramatically when there are many new COVID-19 cases being reported (right, red), likely due to quarantining {%...chose to re-examine our global data at a finer temporal granularity. For each paper, we observed that ...parameters from relatively sparse data from China. An important additional caveat is that our dataset contains...

...by ddPCR begins with diluting the virus. It is important to note that the dynamic range of the ddPCR is...PCR plate. Maintaining a high droplet count is important for calculating the concentration of your sample...separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150 base pairs...

...for further processing. It may be helpful to incorporate regular feeds during this incubation time to ...immunohistochemistry, and immunocytochemistry. It’s important to note that some antibodies are better suited...Rockberg J, Chotteau V. Small-scale bioreactor supports high density HEK293 cell perfusion culture for...

...transduced rat neurons in vivo with beta-gal and other reporters, and they showed that expression persisted for...Figure 2). The HIV genes that do remain are very important for viral production: Gag (structural precursor...activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral envelope protein...

...species, or even an antibiotic resistance gene. Importantly, native, non-pathogenic bacteria remain to help...recolonize the niche, reducing the chance of an opportunistic infection like for organisms like C. difficile...colonization model, phagemid treatment decreased the proportion of virulent S. aureus from 50% to 11% in just...