Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,010 results for: RING;

Showing: 271 - 280 of 1010 results

  1. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ...acceptance addresses were inspiring! We also got to hear more from them during a panel discussion with ...bestowed upon discovers of CRISPR/Cas9 genome engineering, Dr. Jennifer Doudna and Dr. Emmanuelle Charpentier...the presence of scientific heroines is always inspiring. I was especially excited to attend this event...enough to make up for the somewhat naïve questions. During the panel, an artist was sketching her interpretation...accelerate the launch of clinical applications. During the panel discussion, Dr. Mello took it upon himself... in the room. He offered the great analogy of comparing the CRISPR/Cas9 system to Norton Antivirus. Antivirus...

  2. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...of Plasmid Sharing: Interested in more statistics from Addgene's 10 years of plasmid sharing? Check out...our US counterparts are still sleeping). Plasmid sharing in Europe: Addgene’s request statistics Since 2004...Developmental Physiology at the University of Paris VII. During my years in research, I developed several new plasmids...is excited to be working at Addgene, because it brings scientists together from all over the world to ...discussing a new scientific discovery or invention, and sharing those ideas with others. This love of interacting... blog post Or look at our "10 Years of Plasmid Sharing" Infographic!  ...

  3. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... recently showed that by delivering CRISPR components as RNPs and delivering two halves of a repair template... Precision Medicine, CRISPR, and Genome Engineering. Springer, Cham, 2017. 29-42. PubMed PMID: 29130152... used AAV gene editing to introduce naturally occurring cancer-causing mutations into the endogenous allele...homology arms with the modification of interest, ordering them from a gene synthesis company such as IDT...anaphase, and chromosome segregation in human cells harboring tumor-derived mutations in STAG2." PLoS genetics...Howes, Rob, and Christine Schofield. "Genome engineering using adeno-associated virus (AAV)." Chromosomal...

  4. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...goals are (see monitoring task progress), and I know that I’ve blocked out time during my day according...transferable skills while working in the lab and by offering advice on areas where you might need some help... can spend accomplishing small tasks (like... preparing plasmids to send to Addgene). Following my advice...too many activities too close to one another. Monitoring task progress. For this, I always make sure I...-world example, let’s say I’ve blocked out time during my day to write a blog post, edit other blog posts...leave your phone in your bag on “Do Not Disturb” during the times you’ve blocked out for other activities...instance, while I may have found splitting cells boring, hanging out in the tissue culture room also gave...

  5. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...structure determination LEA proteins protect samples during cryo-EM grid freezing Engineered and evolved optimizations...2024.01.21.576499.    LEA proteins protect fragile samples during cryo-EM grid plunge freezing By Rachel Leeson Cryo-EM...can be damaged at the air-water interface (AWI) during the freezing process, resulting in sample loss....protective methods or detergents like CHAPSO, while requiring a lower concentration of sample — nearly ten times...-water interface protectant for fragile samples during cryo-EM grid plunge freezing. bioRxiv 2024.02.06.579238...prime editing systems for specific applications! Delivering prime editors (PEs) via engineered virus-like...off-target editing. Cargo size limits are also less stringent for eVLPs than other viral delivery methods, allowing...

  6. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ... for monitoring RTK signaling.  Figure 4: Design of pYtag fluorescent biosensors for monitoring RTK activation...for investigating neuronal signaling and for engineering improved sensors for these and other targets....class of signaling receptors, but the options for measuring activity of specific RTKs are limited. In a new...colleagues describe pYtags, a modular system for monitoring specific RTK activation in vivo (Farahani et ...sensors. Using genome mining and structure-guided engineering of potassium binding proteins, they developed...and are a promising starting point for further engineering. Figure 5: Structure-guided design and genome...s41467-022-30685-x. Feel the heat with B-gTEMP  Measuring local temperature variations and heat transfer...

  7. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...of animals. There are many different naturally occurring strains of E. coli, some of which are deadly to...strain is a bit more convoluted due to researchers sharing and renaming samples throughout history. It was...cloning, routine cloning, cloning unstable DNA, preparing unmethylated DNA, and more. Many mutations that...Consequence dam DNA adenine methylase mutation (GATC) Preparing unmethylated DNA, important when trying to cut... dcm DNA cytosine methylase mutation (CCWGG) Preparing unmethylated DNA, important when trying to cut...relA1 Relaxed phenotype, mutation eliminating stringent factor Allows RNA synthesis in absence of protein...lacZΔM15] hsdR17(rK-mK+) JM110  Streptomycin  For storing plasmids that should not be Dam or Dcm methylated...

  8. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...those entering science communication (myself included) come from a scientific background and bring with... (and I hope I’m not alone in this sentiment). Entering the final semester of a neuroscience major, I ...first semester in which I had several projects nearing deadline (an animated video depicting nuclear fission...level of career satisfaction indeed.   For many venturing forth from tertiary study, the myriad trajectories... scientific research is, after all, one of the enduring goals of science communication.   The Owheo...improve the world around us has long been known; ensuring all have access to this power and the tools to...

  9. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...technology since its initial applications for genome engineering in 2012.  Many of these advances have stemmed... fold induction at 31X. By using a slit pattern during blue light exposure (470nm), the researchers showed... C-terminal (residues 714-1368, C714) halves, rendering the Cas9 non-functional when split but regaining...strategy which had been engineered from naturally occurring photoactive proteins (i.e. CRY2 and Vivid) - Alexander... details, check out this comprehensive review comparing optogenetic CRISPR techniques (Matony et al., ...Built to Last. In: Methods in Molecular Biology. Springer US, pp 261–281 Manna D, Maji B, Gangopadhyay SA... Bratovič M, Charpentier E, Möglich A (2013) Engineering of temperature- and light-switchable Cas9 variants...

  10. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ... genome engineering, CRISPR is much more efficient at generating the desired mutant offspring, with 85%...adults mated to produce completely transgenic offspring. Although powerful, this technology is cumbersome... Advantages of using CRISPR for mouse genome engineering From the very start, generating mouse models ...microinjectionist, who has the strategically important job of delivering the payload! Last but not least, when using CRISPR...Marraffini LA, Zhang F (2013) Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 339:819–823...Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering. Cell 154:1370–1379 . https://doi.org/10.1016...Multiple Genes by CRISPR/Cas-Mediated Genome Engineering. Cell 153:910–918 . https://doi.org/10.1016/j.cell...
Showing: 271 - 280 of 1010 results