We narrowed to 911 results for: TRO

...eliminate fluctuations due to copy number by introducing genes of interest into the chromosome. But this...input signal that both positively and negatively controls the output. In this case, plasmid copy number ...gene increases its expression. However, if you introduce negative regulation by using a promoter that is.... placed a trans-acting RepA protein under the control of IPTG. Because RepA is a plasmid replication ...Segall-Shapiro et al. placed the genes under the control of a constitutive promoter and then moved them ...decreased. But when the genes were placed under the control of a stabilized promoter, deoxychromoviridans titers...the joys of a p-value < 0.05, having such fine control of gene expression is useful for many metabolic...

...needed and when is it not? What are the proper controls needed to pressure-test your FRET assay to make...pyrrolysyl-tRNA synthetase Mammalian Cells pCMV-DnpK dinitrophenyl hapten Mammalian Cells pANAP AnapRS Mammalian...commercially available as Lumio Green and Lumio Red from Invitrogen (now ThermoFisher Scientific). These reagents...molecular distances (see Table). However, proper control experiments are needed to establish the dynamic...These experiments include producing a positive control construct where the donor and the acceptor fluorophore... each other on the same protein and a negative control experiment conducted on donor-only labeled protein...biarsenical− tetracysteine motif determined by NMR spectroscopy." Journal of the American Chemical Society 131.13...

...All three can introduce multiple base pair deletions, but CRISPR/Cas9 can also introduce point mutations...homologous recombination. A targeting vector is electroporated into embryonic stem cells and this will insert...joining, the newly inserted gene may have errors introduced to the nucleotide sequence during the DNA repair... in more detail in part 2 of this blog series) controls the cell types in which the conditional recombination...engineered mouse model allows gene expression to be controlled through a drug or small molecule that regulates..., Cre is fused to the ligand binding domain of estrogen receptor alpha (Esr1). Mice are fed or injected...

... A fast blue-light inducible Cre for precise control of bacterial gene expression By Meghan Rego Recombinases...recombinases to manipulate genomes, scientists can control expression of their genes of interest. While recombinases...their activity can be fine-tuned and precisely controlled through the use of optogenetics. Optogenetic ...authors demonstrate that CRISPR-Casɸ is active in vitro as well as in human and plant cells. Casɸ...assay for mammalian cells. It's based on the introduction & resolution of two DSBs in a fluorescent reporter...serotype AAV5.  Get the channelrhodopsin here Astrocyte specific tight affinity glutamate sensor from ...from the Sanford Boye lab with enhanced retinal tropism. Find the upcoming serotypes here  ...

... affinity terminator may be desired for multi-cistronic constructs where high termination efficiency is...structure. It is thought that poly(A)s are used to control the cellular concentration of regulatory RNAs and... and may additionally act as a quality control mechanism to rid the cell of mis-folded RNAs. Eukaryotic...virus 40 late polyadenylation signal by using in vitro analyses. Molecular and Cellular Biology. 1992. ...Ther. 2008. PMID: 18627247 Zufferey R, Donello JE, Trono D, Hope TJ. Woodchuck Hepatitis Virus Posttranscriptional...Enhances Expression of Transgenes  Delivered by Retroviral Vectors. Journal of Virology. 1999. PubMed PMID...Resources on the Addgene Blog Learn how the Promoter Controls Gene Expression Learn about New Cloning Techniques...

...stimulation itself, the output of the laser must be controlled. As discussed above, each opsin variant has slightly...the A.M.P.I Master-9 Pulse Stimulator, which can control output of several lasers simultaneously, can be.... Pulse generation (TTL pulse) is a standard electronics protocol not specific to lasers or optogenetics...powerful alternatives to the Master-9 that only control one or two lasers are also available. Optimal stimulation...loss of laser power. More recently, wirelessly-controlled optogenetic implants have been reported by several...References 1. Gutierrez DV, et al. Optogenetic control of motor coordination by Gi/o protein-coupled vertebrate... Kim TI, et al. Injectable, cellular-scale optoelectronics with applications for wireless optogenetics...

...do not let the panelists introduce themselves. I do a 1-2 minute introduction. If you chose good panelists...and will play a different role so her ability to control the event is what is important. Choose 4 panelists...logistics To make a really lively discussion, I strongly suggest asking the panelists not to use any slides...it (lapel mics are best). The moderator takes control It is the moderator's job to prevent anyone from...talking, so they will easily take 5 minutes to introduce themselves and, voila, 1/2 of your time is gone...publicity. Good panels do not waste time on long introductions. The moderator asks questions, calling on 1-...

... of plasmids, they are generally electroporated into electrocompetent cells to ensure the highest possible...chemical transformations is less than that of electroporation, but an advantage is that chemical transformations...protocol, the size of the cuvettes your lab’s electroporator uses, etc. The DNA amount can be anywhere from...DNA in 100 μL of cells over four individual electroporations. The specifics of this can be based on the...5,000,000 30 bp At least 200 ng Liquid Electroporation Protocol (DOCX, 15.8 KB) Barcode SPLINTR... CRISPR Preparing Pooled Libraries NGS Quality Control for Pooled Libraries Resources on Addgene.org Addgene's...

...SARS-COV-1 plasmids. Export control license process    An export control license, once approved, allows...repository, including rabies plasmids, are export-controlled materials in the USA, where Addgene is located...applicable research project when purchasing export-controlled materials. Addgene will then apply for the license...   Due to recent regulation changes, an export control license for rabies materials may be required for...application process.  More information about export control licenses is available through the US Bureau of ...

... they then confirmed by PCR. Subsequent mass spectroscopy studies allowed for the identification of proteins...Fujii Lab generated a transgenic mouse line and retroviral expression system for using this approach in ...analyzed, the Fujii lab also developed another "in vitro" form. In this form, the CRISPR complex, consisting...biotinylated gRNA, were successfully used in this "in vitro" form. Any tag system can be used to isolate genomic...expressed can cause facioscapulohumeral muscular dystrophy (FSHD), a disease for which there is currently... isolation of DNA fragments and chromatin by in vitro enCh IP technology using recombinant CRISPR ribonucleoproteins...as an RNA-guided platform for sequence-specific control of gene expression." Cell 152.5 (2013): 1173-1183...