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We narrowed to 885 results for: TRI

Showing: 541 - 560 of 885 results

  1. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...These modifications result in conformationally restricted RNA which improves mismatch discrimination and... specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce the innate immune response...

  2. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...approaching it. These include the traditional restriction enzyme/ligase-mediated method, the more recently.../19 vectors and demonstrating the concept of restriction enzyme-mediated disruption of ccdB, leading to...

  5. Lab Automation at Addgene

    Type
    Blog Post
    ... to prep large quantities of DNA because some countries just can’t receive bacteria through the mail due..., if you have any great lab automation tips and tricks, be sure to share them in the comments section....

  8. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...efficiency and any unpaired fluorescent proteins can contribute additional noise to the measurement. If the distance... FRET experiments are expected to continue to contribute to our understanding of diseases and even assist...

  9. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...green, blue, red, and far-red. There is also a ratiometric GECO sensor (GEX), where one wavelength changes... working with a stably integrated biosensor. Ratiometric measurements with certain single FPs (such as...

  10. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...References and resources References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application...(08)60255-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary...

  11. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...although both are of course preferred! When trying to strike that balance, you may wonder: how close is close...significant amount of flexibility compared to a strict single-compatibility sequence like NGG. Nonetheless...

  12. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...laboratory of Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will... function in receiver cells. There are over 40 trillion cells that make up the human body and G-baToN ...

  13. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...photostable YFP to date. As such, mGold is expected to contribute to experiments that require high photostability...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth...

  14. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...plays an important role in RNA processing and contributes to variability in RNA half-life, and ultimately... Rho-independent termination is also known as intrinsic termination, and it relies on the formation of...

  15. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...in-situ hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase...Cardiovascular and diabetes mortality determined by nutrition during parents' and grandparents' slow growth ...

  16. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...This post was contributed by Brittany L. Uhlorn, a PhD Candidate at the University of Arizona. Perhaps... next poster session! There many other tips and tricks for presenting at scientific conferences, so let...

  18. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...standard curves, and normal controls must be run in triplicate.  The nice thing about qPCR is that since it ... of reactions, it does not need to be run in triplicates. ddPCR is an extremely accurate application, ...

  19. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...This post was contributed by guest blogger Iris Lindberg, Professor at the University of Maryland School...Cloning cells using an agarose overlay does have one tricky step. It is necessary to pour the agarose onto ...

  20. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...This post was contributed by guest bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss...relative nuclease efficiencies as well as to more stringent targeting rules that result in a decrease in available...
Showing: 541 - 560 of 885 results