We narrowed to 928 results for: INA

1. Six Spooky Science Stories and Halloween at Addgene

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   Blog Post

   ...Blugene made an appearance there too) and shared fascinatingly creepy science stories. Here are some of our...

2. IDP and your PI: A Roadmap for Career Planning and Personal Development

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   ...accomplishments, from learning new techniques to finally figuring out a tough experiment, sets a positive...

3. Science as Art

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   ...plants and other organisms without destroying the original, which could provide a new tool to study old and...

4. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

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   ...creating deletions, identifying these deletions, and finally characterization. Read and download the full protocol...

5. What's New in CRISPR - September 2019

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   ...unwinding. They’ve deposited plasmids for active and inactive CasX for E. coli and human expression. Read the...

6. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

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   ...testing - and repeat - we’re proud and excited to finally share with you what we’ve been working on behind...

7. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

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   ...reagent, e.g., antigen retrieval conditions, dye inactivation details, tissue preservation method, and detergents...

8. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

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   ...been working with members of Davidson's lab to coordinate the impressively big deposit. “He has every fluorescent...

9. In Living Color: The Skinny on In Vivo Imaging Tools

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   ...toolbox, which increase their fluorescence upon illumination with far-red light. As the researchers described...

10. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

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    ...These pooled libraries include enrichment against kinases, cell cycle proteins, and more.  “In the past, ...

11. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

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    ...Check out our Plasmids 101 featured topic page Additioinal Resources on Addgene.org Molecular Biology Reference...

12. Fostering communication in remote collaborations

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    ...for incorporating everyone’s suggestions into a final version of the document. Set and manage expectations...

13. Some Like it Hot: Thermostable GeoCas9

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    Blog Post

    ... require high temperature reaction conditions. Finally, its longevity in human serum may allow for more...

14. SunTag and Fluorescent Imaging

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    Blog Post

    ...This workaround risks cellular phototoxicity and eliminates the possibility of long-term imaging studies....

15. CASTing Off for New Shores in Human Genome Editing

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    ... combines prime editing with a site-specific recombinase to achieve insertion of long sequences. In their...

16. Build Your CRISPR Vocabulary

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    ... with alternating spacers and repeats, was the original impetus for studying CRISPR. Spacers: Copies of...

17. Antibodies 101: Affinity Tags

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    ... it is still bound to the glutathione resin, eliminating the need for an extra clean up step of the tag...

18. Plasmids 101: Gene, enhancer, and promoter trapping

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    Blog Post

    ...measure the function of a gene. For example, they eliminate the expression of the gene (by CRISPR knockout...

19. Year of the Camelid: Antibody Style

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    Blog Post

    ...alpacas). But because sdAbs are so amenable to recombinant expression (and because maintaining a llama facility...

20. Running for Rare Disease, Running for FOP, Running for AJ

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    ...antibody that binds Activin A and interferes with inappropriate activin-ACVR1 interactions prevents FOP bone...

21. Addgene at the ASM Microbe 2016 Conference

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    Blog Post

    ...Advisory Board member Siouxsie Wiles gave us a fascinating and fun look at her outreach work with fluorescent...

22. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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    ...land a position you’ll be happy in once you’ve finally acquired that PhD. Resources at Addgene Read ...

23. Management for Scientists: What Makes a Good Manager Anyway?

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    ...prefer to be approached as peers rather than subordinates Need mental space and dislike intrusions (this...

24. Choosing Your Perfect Empty Backbone

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    ...all out with constitutive high level expression? Finally, you can add bells and whistles to your construct...

25. Advice for Choosing a Research Project

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    ...the research project must have innovative yet attainable milestones that contribute to intellectual thought...

26. Choosing a Good Mentor for Scientists

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    ...many aspects to choosing the right labs (see my webinar (updated link from 2020) on this topic), for this...

27. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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    ...are free for K-12 teachers and available for a nominal fee for university professors, are currently available...

28. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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    Blog Post

    ...reporting in the discipline and reduces the dissemination of unwarranted claims or unacceptable interpretations...

29. Fluorescent Biosensors for Measuring Autophagic Flux

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    Blog Post

    ...however, can not be conjugated to PE due to a C-terminal glycine deletion, so it remains in the cytosol...

30. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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    ...have no effect on the purity or infectivity of the final viral preparation. Through sugar detection assays...