We narrowed to 922 results for: POR

1. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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   Blog Post

   ... source library of characterized aptamers and supporting publication: http://www.freebase.com/base/aptamer...

2. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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   Blog Post

   ...many of them very popular. Why has it been so important to you to distribute these tools as freely as ...

3. Hot Plasmids - January 2023

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   Blog Post

   ...ubiquitous promoter drives expression of an mCherry reporter.  After using mCherry for FACS sorting of the ...

4. The Power Behind NGS Plasmid Validation: seqWell

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   Blog Post

   ...proprietary chemistry). This turns out to be critically important for getting adequate sequencing depth and coverage...

5. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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   Blog Post

   ...equipment, and expected DNA concentration. It’s important to consider the advantages and disadvantages of...

6. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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   Blog Post

   ...session you’re presenting is eye contact. “It’s important to keep your video on and look into the camera...

7. Visualizing Genomic Loci with CRISPR-Sirius

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   Blog Post

   ...robust visualization tool to study the spatial and temporal arrangement of chromosomes and how these arrangements...

8. Improving Transduction Efficiency with Exosome AAVs

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   Blog Post

   ...retina upon intravitreal injection." Scientific reports 7 (2017): 45329. PubMed PMID: 28361998. PubMed ...

9. Plasmids 101: Gram Negative and Positive Bacteria

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   Blog Post

   ...inserted into a specific bacterium, it can be important to consider the type of bacteria it is being used...

10. DAP arrays for multiplex gene editing

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    Blog Post

    ...Multiplex expression of gRNAs Four Base Editing Reporters to Monitor and Enrich Editing in Real Time Prime...

11. Celebrate National Book Month with Addgene

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    Blog Post

    ...Microbes are everywhere and this book emphasizes the important roles microbes play in our ecosystem, examining...

12. New and Upcoming Viral Vectors - September 2019

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    Blog Post

    ...intersectional approaches is a growing field that we aim to support by growing our inventory of recombinases and recombinase-dependent...

13. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    Blog Post

    ...Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven ChRs derived...

14. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    Blog Post

    ...photosensitizer fluorogenic dye.  MG-2I is the TAP portion of the complex and is derived from the malachite...

15. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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    Blog Post

    ...you to systematically study gene function with temporal control and cell-type specificity! The Gal4/...

16. Synthetic Photobiology: Optogenetics for E. coli

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    Blog Post

    ...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates...

17. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    Blog Post

    ...fluorescent proteins with this filter set and have reported brightness measurements. Here, EGFP outperforms...

18. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post

    ...cloning: A SLIC makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen ...

19. Flow Cytometry Readouts: Yes, No, and Everything in Between

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    Blog Post

    ...populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk you through how ...

20. Antibodies 101: Flow Cytometry Controls

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    Blog Post

    ...point for compensation (Fig. 1B-C). However, it’s important to note that because you’re using beads and not...

21. Viral Vectors 101: Biosensors

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    Blog Post

    ...phenomena by generating an output which is directly proportionate to the amount of a given factor within a biological...

22. Viral Vectors 101: Types of viruses

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    Blog Post

    ...Lenti particles enter the nucleus through nuclear pores and thus do not require breakdown of the nuclear...

23. Top Requested Lentivirus and AAV of 2016

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    Blog Post

    ...of transduction efficiency, tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse...

24. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post

    ...localization signal (NLS). In a HEK293 mCherry reporter assay, CasRx and AdmCas13d produced 92% and 87%...

25. 15 Years of Addgene: The Top 15 Plasmids

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    Blog Post

    ...requestors for making their reagents available and for supporting scientific sharing. We’re very excited for more...

26. Antibodies 101: ChIP

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    Blog Post

    ...agitation; a shorter sonication time could be an opportunity to decrease crosslinking. (Homogenization for...

27. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post

    ...whenever you use the antibody. This information is important, and you’ll need to track it throughout your experiments...

28. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    Blog Post

    ...signaling and inflammatory responses remotely with spatiotemporal precision. This is a great tool to regulate...

29. Plasmids 101: Methylation and Restriction Enzymes

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    Blog Post

    ... directed mutagenesis. During this process, incorporation of a desired mutation into your plasmid of interest...

30. How to Keep a Lab Notebook for Bioinformatic Analyses

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    Blog Post

    ...your methods section about the edit that was temporarily used. Just be sure that the original code remains...