We narrowed to 910 results for: ire;

1. Addgene Welcomes John Doench to the Advisory Board

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   Blog Post

   ...to the Advisory Board. John Doench is Associate Director of the Genetic Perturbation Platform at the Broad...

2. Resume Writing for Non-academic Science Careers

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   Blog Post

   ...Experimental Medicine. She is the Community Relations Director for Curiosity Collider (hyperlink https://curiositycollider.org...

3. 15 Years of Addgene: The Top 15 Plasmids

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   Blog Post

   ...lab that contains the gag and pol genes. It also requires pRSV-Rev and the envelope expressing plasmid pMD2...

4. Early Career Researcher Toolbox: Social Media for Scientists

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   Blog Post

   ...results we hoped for, but it did open up a new direction for the project. I was invited to participate ...

5. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   Blog Post

   ...plasmids, glycoproteins and other proteins that require post-translational modification may not be easily...

6. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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   Blog Post

   ...Plasmids for Subcellular Localization Browse the entire Fluorescent Protein Collection ...

7. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   ...mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs from Bryan Roth’...

8. 7 Tips to Secure a STEAM Internship This Summer

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   Blog Post

   ...Furthermore, companies across most industries tend to hire their former interns to fill entry-level positions...

9. Behind the scenes: Addgene’s new search engine and more

   Type

   Blog Post

   ...are many ways a user can input their query — by directly typing terms in a search bar, by ticking some ...

10. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type

    Blog Post

    ...proteins. Affinity purification methods typically require a protein to be tagged, which limits these approaches...

11. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post

    ...Although this method shortens construct size, it requires coexpression of Csy4 with Cas9 and the polycistronic...

12. How to Make Friends and Meet People at a Scientific Conference

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    Blog Post

    ...the person you are talking to is really in your direct area.  Review the conference schedule and attendance...

13. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post

    ...is helpful to find peers that share a similar direction or problem. For example, a group of people who...

14. Plasmid Tools for Microbiome Studies

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    Blog Post

    ...transfer origin as well as a selectable marker and a desired genetic payload (ex: GFP). Thus, cells can be both...

15. dTAG - You're it!

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    Blog Post

    ...Models CRISPR 101: Cas9 Nickase Design and Homology Directed Repair Plasmids for endogenous gene tagging in...

16. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post

    ...endothelial cells from women who are missing an entire X chromosome (TS iPSCs), the lab observed abnormal...

17. Addgene: The First Twenty Years

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    Blog Post

    ...to be young again! Addgenie Marcy (now our Sr. Director of Production) pie-fully celebrating Addgene's...

18. Transferable Skills Guide: Public Speaking

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    Blog Post

    ...collaboration and growth opportunities, and gives your entire audience a chance to comfortably engage with you...

19. Antibodies 101: Conjugation

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    Blog Post

    ... 2012). And, you can also conjugate antibodies directly to beads for purification purposes.  Coupling...

20. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post

    ...chemical modifications to the RNA to control the entire editing system with a simple LED light. Lighting...

21. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    ...not so simple Just as our lab used jargon that required context to understand, I came to realize that ...

22. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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    Blog Post

    ...it would take years to develop the mouse lines required for this purpose. Tang wondered if he could use...

23. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    Blog Post

    ...Scientific Manuscripts. Any scientific manuscript will require numerous other references to scientific literature...

24. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type

    Blog Post

    ...GFP.   Figure 2: Synthetic promoters can be paired for targeted expression. This same pairing of...

25. Corralling and Cornering the Goldilocks Antibody

    Type

    Blog Post

    ...perform differently over time), compatible with the desired applications (such as immunohistochemistry (IHC...

26. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    ...al., 2019). Finally, scientists can perform the entire process of antibody selection and maturation in...

27. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    ... order. (It’s also why you can’t skip a module entirely, but instead will have to replace it with a spacer...

28. Early Career Researcher Toolbox: Finding Relevant Papers

    Type

    Blog Post

    ...This is a great way to have new papers delivered directly to your inbox. Best uses Staying up-to-date on...

29. Tips for Technical Support Calls

    Type

    Blog Post

    ...end of that email you’re writing is probably not directly responsible for the problem. We’re here to help...

30. Illuminating Epigenetics with A FRET Based Biosensor

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    Blog Post

    ... has recently been hitting the headlines, with sotires like the potential devastation of the palm oil ...