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We narrowed to 970 results for: Abo;

Showing: 541 - 560 of 970 results

  1. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...clinical settings. AAV is also a cool tool in the laboratory! It is ideal for whole animal administration,... be helpful to you. Interested in learning more about a specific virus? We have you covered with guides..., adenovirus, and AAV. If you’re wondering more about what applications these viruses are good for, we...

  2. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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    Blog Post
    ...off target effects. However, these methods can be labor intensive and quite time consuming.   As Cas9/gRNA...have been several systems to do this but most are labor-intensive, require multiple fluorescent proteins... Browse all of our CRISPR blog posts Find more about fluorescent proteins Get a refresher on CRISPR with...

  4. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...this multidisciplinary effort and cross-team collaboration (thanks to all Addgenies involved). The new ...Additional resources from the Addgene blog Learn about other features on Addgene's website Find a step-...to using Addgene's new search Read to learn more about why we redesigned our homepage and mascot ...

  5. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...chemogenetics  While there’s still a lot to learn about GPCR signaling, researchers in the meantime have... inhibit neurons. You can find information more about chemogenetics and a concise table of DREADDS and...Additional resources on the Addgene blog Learn more about TRUPATH Read our quick guide to chemogenetics Resources...

  6. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...Rose is passionate about outreach among low-income communities to educate parents about resources that can...automated filters of consideration. If you are worried about any eligibility provision, don’t guess. Simply reach...

  7. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...blog Learn about other CRISPR methods for bacterial genome engineering   Learn more about lambda red ... genetic variants in bacteria. Read on to learn about the key components of EvolvR and its potential applications...

  8. Antibodies 101: Conjugation

    Type
    Blog Post
    ...Check out our post on Biotinylation to learn more about biotin and its use in molecular biology. Other Conjugates...perfectly blissful, productive lives without thinking about these chemical reactions. After all, there is an...ready to use off the shelf. So why are we talking about this? Even though there are so many commercially...

  9. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...Deficiency (CTD) is a rare X-linked inherited neurometabolic condition and one of three Cerebral Creatine... into CCDS. The brain and muscles have a high metabolic demand that is powered by ATP. A molecule central...Hébert's lab. Additional Resources Find out more about the Rare Genomics Institute and this year's BeHeard...

  11. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post
    ...“I hope it isn’t degraded” when scientists talk about their precious RNA samples. RNA is inherently less...efficacy as well as the unnecessary stress of worrying about the status of your RNA. gRNAs modifications are ...Some of the RNA stabilizing modifications listed above were developed before CRISPR-Cas9, for use in applications...

  12. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...Narrowing the field After working your way through the above steps, you may find that you still have a number...advice if you need to troubleshoot. What do you know about the vendor and the vendor’s technical support? An...validation is equal and you should think critically about what data is and is not shown on an antibody website...

  13. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...make AAV ideal for human therapies and in vivo laboratory experiments ranging from transient gene expression...genetic cargo. Typically, when researchers speak about AAVs, they are actually referring to rAAVs. ITRs...Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources on Addgene.org ...

  14. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ...which can be used with this approach - read more about them on our CRISPRa blog! Fig. 1 CRISPR ... is a lot of overlap with the systems discussed above, just with more elements of control. Tet promoters...to be site-specifically knocked in. This can be labor intensive and time consuming. If your native protein...

  15. CRISPR 101: Cas9 vs. The Other Cas(s)

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    Blog Post
    ... is smaller than the traditional SpCas9 enzyme (about a third of the size) and does not require a tracrRNA...Cas12a to further optimize it for therapeutic and laboratory usage. Fast facts – PAM requirement: TTN or ... the lack of collateral cleavage). You can read about Cas7-11 in our blog post on its development and ...

  16. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...both quantitative and spatio-temporal information about the turnover of specific endogenous proteins in ... interested in neurobiology.  She is passionate about communicating science to all audiences. Right now...

  17. RNA Extraction Without A Kit

    Type
    Blog Post
    ...more foolproof (still without a kit!) As mentioned above, working with RNA requires keeping your samples ...lysis. This can be challenging depending on your laboratory situation or tissue collection method, so biotechnology...supernatant is extracted with chloroform as in the above no-kit method.  Researchers have also developed ...

  19. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...  Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...Once the initial antibody response starts to wane about a month later, the animal is given additional booster...

  20. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...With this new found structural knowledge, in collaboration with the Joung Lab, they used targeted mutagenesis...edits in mammalian cells and may decrease worries about off target effects in applied and/or therapeutic...at Addgene. Resources on the Addgene Blog Read about Cas9 Homolog Cpf1 Read How Non-Homologous End Joining...
Showing: 541 - 560 of 970 results