...reliable sequence data back for the first 50-100 base pairs of your sequencing read. You can expect to...mammalian selection markers. You don’t need a fancy database to organize your own plasmid collection, although...help of DNA-editing software which integrates our database of over 2000 primers. Two 96-well PCR plates are...Finally, you will want to have a well-organized database of expected sequences, either for the inserts ...Get Plasmid Backbone Information from Our Vector Database  ...

...This article is based on an interview with Novartis researcher, Carrie Bhang. The ClonTracer Library,...

... CRISPR guides that flank regions of hundreds of base pairs or more can simultaneously introduce a pair...

...marker help synthesize? Counterselection? (growth-based positive selection for the loss of the marker gene...

...Modified Constructs The genetic code for all life is based upon four nucleotides, 64 codons, and 20 amino acids...acid would be incorporated. Other options, such a 4-base pair codons, have also been utilized. A tRNA that...

...solution will drift rapidly upon addition of acid or base. Add only a few drops at a time, allow them to mix...

...procedure for separating DNA by size (e.g., length in base pairs) for visualization and purification. Electrophoresis...

..., Portugal. She leads the Glycoimmunology group based at the Research Unit on Applied Molecular Biosciences...

...difficult for individual labs to complete. The nanobody based RANbody platform from the Sanes Lab overcomes this...

...got a full time position with a Washington D.C.-based communications consulting firm, Palladian Partners...

...with BioRender.com. To make channelrhodopsin-based optogenetics minimally-invasive, Frances Arnold’...

...and RNAProtect® from Qiagen, are ammonium sulfate-based solutions that work by inhibiting RNase activity...

...distinguished. The UCLA Library offers some Powerpoint based templates with different column numbers for a good...

... at present.   Another issue surrounding CRISPR-based therapy is the level of testing needed to define...

...Gγ). G proteins are divided into four subtypes, based on their Gα subunit: Gαs, Gαi/o, Gαq/11, and Gα12...

...and optimization of a real-time quantitative PCR-based method for the titration of AAV-2 vector stocks....

...beyond generating frameshift mutations or single base pair edits, these enzymes are worth taking a look...Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction. Cas3 is thus not helpful...

...provide recombinant antibodies, another plasmid-based product that, with Addgene’s support, could fill...

...having effects on various biological disciplines, the base technology itself is constantly improving. Cas9 ...

...References 1.Tang, Jonathan C.Y., et al. “A nanobody-based system using fluorescent proteins as scaffolds for...