We narrowed to 641 results for: CAR
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TypeBlog Post...achieve greater efficacy, researchers will have to carry out repeated transfections with the ssDNA guide,...
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3D Printing Meets CRISPR Cas9
TypeBlog Post...now have another model of Cas9 – a flexible alpha-carbon backbone model made of nylon – that more accurately... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog Post...your mutation. To maintain stable lines of flies carrying a specific mutation, Drosophila geneticists use... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...PMCID: PMC3074624. 5. Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent protein for... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...reviews journal. She joined Cell Press to pursue a career in science communication and publishing after completing... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog Post...could be forming dimers in cells. Why should you care? Three simple ways a dimeric FP could ruin your ... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...Pubmed. C. Soderblom, D.-H. Lee, A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2)... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog Post... Caroline LaManna... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post...Golden Gate Modular Cloning Toolbox for Plants. Carola Engler, Mark Youles, Ramona Gruetzner, Tim-Martin... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...many genes simultaneously. These parts have been carefully engineered to support reliable functional composition... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...Ampicillin VWR: #7177-48-2. Use at 100 μg/mL. Carbenicillin VWR: #80030-956. Use at 100 μg/mL. C.2 Annealing...agar plates containing 100 μg/mL ampicillin or carbenicillin (an ampicillin analog). Due to the long terminal... ligation into LB + 100 μg/mL ampicillin or carbenicillin. Day 2: 2. Spin down the cultures and use a ... to 55°C. Add 1mL of 100mg/mL ampicillin or carbenicillin to obtain a final concentration of 100 μg/mL...distribution of any literature by Addgene is not meant to carry with it, and does not grant any license or rights...distribution of materials by Addgene is not meant to carry with it, and does not grant any license, express... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog Post...Mozziconacci O, Schöneich C, Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock... -
An Introduction to Adenovirus
TypeBlog Post...2007-02-062117. PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, J. S., Wold, L. ... -
Getting to Know Expansion Microscopy
TypeBlog Post...distortions to a minimum with protocol optimization and careful execution, but they cannot be entirely eliminated... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...hours, so read up on the timing of this approach carefully (Lin et al., eLife)! Use a Cas that facilitates... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...how will you use these systems in your research? Cary Valley contributed to updating this article. References... -
Droplet Digital PCR for AAV Quantitation
TypeBlog Post... manual droplet generator, you will have to be careful about transferring your droplets from the droplet... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog Post...with a reporter enzyme. For multicolor staining, careful consideration must be used when selecting which... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...below. The main requirement for IVA cloning is carefully designed primers. The majority of mistakes are...