We narrowed to 821 results for: EED

...Ligand regulated recombinase - keeping Cre out until needed But what can be done when the recombination should...induced by Cre recombinase expression while proteins needed for later experiments (cellular tracking for instance...

...host cell division to plasmid proliferation." Proceedings of the National Academy of Sciences 80.15 (1983... model for programmed bacterial cell death." Proceedings of the National Academy of Sciences 93.12 (1996...

... neuronal synapses for instance. Also these tags need to be fused to scaffold proteins in order to be ...composed of FPs fused to multiple epitope tags. Indeed, to create the smFPs Looger’s team strategically...

... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...bistable network, stripe pattern-forming incoherent feed-forward loop. Find the plasmids at Addgene! ...

.... But in order to plan a flow experiment, you’ll need to first read papers with flow data... and to read...endless loop. Let’s break out of the loop with all you need to know on how to read a flow plot.  Dot plots ...

... adjacent motif) near the target DNA, TnpBs also need a short sequence motif called a TAM (transposon-... used to generate gene-edited plants without the need for tissue culture (Maher et al., 2020; Qiao et ...

...processes in C. elegans: mScarlet3 for applications needing intense brightness (such as low-expression proteins...co-imaging. And because bioluminescent markers do not need to be excited by an external light source, they ...

...viral vector selection process to standardize our feedback and evaluation of viral vectors. This process ...as they've helped us.  Interested in providing feedback on AAVs at Addgene? Suggest a viral vector for...

... with WPRE reduced the number of copies of gvpA needed to increase expression. We were able to express...microscopy and TEM (Fig. 2c), and ultrasound imaging. If needed, resort the cells again for improved purity and...

... noticed Holly Bik live-tweeting about topics I needed to know more about. I caught up with her outside...Some of the variability was organic, as some people need less assistance than others, but we did have some...

...companies produce different products and therefore need people with different areas of expertise in technical...unobtainable solutions. To some extent there is the need (or opportunity) to get involved in product testing...

...or C-terminal fusion based on your experimental needs. Equally important, before cloning you must consider...dark fusions is likely misfolded, blocked from proceeding through the secretory pathway, and retained in...

...neuronal activation and is a sufficient readout to proceed with your behavioral experiments. Comparison of... to use c-fos expression to get the evidence you need. For example, as shown in this study [3], hippocampal...

...commercial strains designed to avoid them. Why do I need an expression strain? Protein expression from high-copy...number plasmids and powerful promoters will greatly exceed that of any native host protein, using up valuable...

...their biotinylation. The advantage of APEX is its speed, labeling neighboring proteins in minutes rather...developed in the Ting Lab in 2018 to increase the speed of proximity labeling by promiscuous biotin ligases...

...In the mVenus-APT1, a lipidation motif, which is needed for Golgi localization, is occluded and therefore...circularly permuted yellow fluorescent proteins." Proceedings of the National Academy of Sciences 101.29 (2004...

... A,T or C – but avoid NAG) or guide RNA target (“seed”) sequence. The idea was that these “blocking mutations...locus you are editing, and if the blocking mutation needs to be silent. In our view, it’s always best to change...

...recruitment of katanin to that anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module...many tools available for controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and...

...Lentiviral vectors can be produced easily, without the need for time-consuming purification processes, and are...delivered to cells, the viral life cycle initially proceeds as usual. Reverse transcription of the transgene...

... expression levels of your gene of interest, as needed. Controlling the expression levels of your gene...approach is that, since there is no selection, you will need to screen somewhere between one hundred and one ...