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  2. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...2014). Scientists from the University of Geneva (Creed et al., 2015) used optogenetics to support these...Psychoneuroendocrinology 46 (2014): 78-87. PubMed PMID: 24882160. Creed, Meaghan, Vincent Jean Pascoli, and Christian Lüscher...

  4. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ... gene Y, which has a defective allele Ym and you need to convert an adenine (A) to a guanine (G)? You ...with the endogenous tRNA-processing system." Proceedings of the National Academy of Sciences 112.11 (2015...

  6. Hot Plasmids - August 2020

    Type
    Blog Post
    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...bistable network, stripe pattern-forming incoherent feed-forward loop. Find the plasmids at Addgene! ...

  9. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...processes in C. elegans: mScarlet3 for applications needing intense brightness (such as low-expression proteins...co-imaging. And because bioluminescent markers do not need to be excited by an external light source, they ...

  10. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...viral vector selection process to standardize our feedback and evaluation of viral vectors. This process ...as they've helped us.  Interested in providing feedback on AAVs at Addgene? Suggest a viral vector for...

  11. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    .... But in order to plan a flow experiment, you’ll need to first read papers with flow data... and to read...endless loop. Let’s break out of the loop with all you need to know on how to read a flow plot.  Dot plots ...

  12. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...you may even wish to generate a point mutation and need to focus your attention to that particular region...easier and doesn’t require the extra precautions needed when dealing with RNA (e.g. face mask, and a dust...

  15. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...commercial strains designed to avoid them. Why do I need an expression strain? Protein expression from high-copy...number plasmids and powerful promoters will greatly exceed that of any native host protein, using up valuable...

  18. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...recruitment of katanin to that anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module...many tools available for controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and...

  19. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...Lentiviral vectors can be produced easily, without the need for time-consuming purification processes, and are...delivered to cells, the viral life cycle initially proceeds as usual. Reverse transcription of the transgene...
Showing: 541 - 560 of 816 results