We narrowed to 629 results for: EMB

1. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   ...Addgene as a DNA Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the...

2. Corralling and Cornering the Goldilocks Antibody

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   ...Zhuhao Wu and his colleagues! Dr. Wu and his lab members (like me!) are dedicated to creating a public and...

3. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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   ...your standard curve to plot to a linear equation (remember y = mx+b?). Figure 5: A standard curve ...

4. CRISPR in the Clinic

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   ... in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail therapy...

5. Viral Vectors 101: Virus Safety

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   ...biosafety cabinet. When decontaminating liquid waste, remember that the percentage indicates the proportion of...

6. Antibodies 101: Choosing the Right Isotype

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   ..., G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical Techniques for Light and ...

7. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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   ...splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 Zibitt MS, Hartford...

8. 3D Printing Meets CRISPR Cas9

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   ...this surface”….where the complex is docked on the membrane of the endoplasmic reticulum. And graduate students...

9. Truncated gRNAs for Regulating Gene Expression

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   ... environments.     Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board. His laboratory...

10. Addgene's Cancer Collection Pages Connect the Research Community

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    ... We invite feedback and contributions from all members of the research community as we continue to to ...

11. Experimenting with New Careers while in Grad School

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    ...positive. I went back into lab the next day and remembered why I still love science. Keep building on your...

12. Anatomy of a Plasmid Page at Addgene

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    ...provides useful information about how the vector was assembled, and enables requesting labs to utilize and modify...

13. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    ...camouflage. An anemone’s red colored tentacles resemble worms which attract fish for them to prey upon...

14. Transferable Skills: Negotiation

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    ...colleagues and supervisors and lead to you being remembered as a person who tries to find solutions that ...

15. Hot Plasmids: Summer 2025

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    ...allows VAMP2-At to remain on the presynaptic cell membrane while AF, fused to a payload, is released into...

16. Common Injection Routes in Mice

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    ...When considering what method you’d like to use, remember that the final decision will likely come down ...

17. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    ...principle, Peters and colleagues constructed a 40-member library targeting 20 genes of Enterobacter cloacae...

18. Pooled Library Amplifications

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    ... protocols attached.  Final step: validation  Remember, there is no “one-size-fits-all” protocol! Each...

19. Antibodies 101: Flow Compensation

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    ...But we hope this guide can help you get started. Remember, you can always change your compensation values...

20. Of Myc and Men

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    ...mapping functional domains of Drosophila hsp 70. Embo J 3:3087–3093. https://doi.org/10.1002/j.1460-2075.1984...