We narrowed to 638 results for: Erf

1. Light Sheet Fluorescence Microscopy

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   ...scanning microscopy . Since image acquisition is performed using cameras with electron multiplying couple-charged...

2. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   ...starving. I think plant biology is massively underfunded considering how important it is. I would really...

3. Which Fluorescent Protein Should I Use?

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   ...it is translated to a few hours. For example, superfolder GFP (sfGFP) and mNeonGFP can fold in <10min at...

4. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...should be free to use and used for good. It's wonderful to have Addgene as a professional public-benefit...

5. AAVs in Retinal Gene Therapy

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   ... the cell’s nucleus. But since nothing’s ever perfect, neither is AAV. Its main limitation is the small...

6. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   ... DNA with an electrical jolt and cells become perfectly capable of synthesizing fluorophore fusions on...

7. AAVs CREATed for Gene Delivery to the CNS and PNS

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   ...neurons (figure 4). These capsids can serve as powerful cell-type-restricted gene vectors for neurons,...

8. MXS Chaining

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   ...of fluorescent proteins and the availability of powerful imaging technologies, the combination of multiple...

9. SciComm: Taking Science to Elementary and Middle Schools

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   ...students each, and each group went on its own wonderful tangents. Some groups asked about vaccines, others...

10. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ... PCR/sequencing While CRISPR-transposons are powerful and simple to use, users should keep aware of ...

11. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...of your PIPs in which the protein-interaction interface has been compromised (Kodama and Hu, 2012). Such...

12. Great Results Start with Great Standard Curves

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    ...trendline. R2 ranges from 0 to 1 with 1 being a perfect fit and 0 indicating that there is no linear relationship...

13. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    ..., Diaz MFP, Yong WH (2018) An Introduction to Performing Immunofluorescence Staining. Methods in Molecular...

14. Getting to Know Expansion Microscopy

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    ...can achieve. This means that regardless of how powerful of an objective you have, you can’t resolve two...

15. CRISPR 101: Making a Knock-In Cell Line

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    ...increase HDR frequency by committing a break to performing HR before other factors were loaded onto the ...

16. Uncovering Ancestral Bias in CRISPR Research

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    ... screens they analyzed were designed to have a perfect match to the reference genome – even single mismatches...

17. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    ... can be stored right away. Every week, the lab performs an average of 140 transformations. The lab typically...

18. Plasmids 101: Protein tags

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    ...co-IP, IF, flow -cytometry; can occassionally interfere with protein folding HBH HHHHHHAGKA GEGEIPAPLA...

19. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method to suppress PCR amplification...

20. Negotiating Work and Life: How to Find the Joy

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    ... and/or your spouse won’t be at every school performance, chaperone every field trip or cheer at every...