Addgene

We narrowed to 632 results for: Erf

Showing: 541 - 560 of 632 results

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Tips for a 1st time AAV user (by a Rookie AAV user)

Type

Blog Post

...found in the culture media, you should consider performing PEG precipitation. Instead of sonicating the ...
PCR Applications for Copy Number Variation Assays

Type

Blog Post

... quantification. Even though no one option is perfect, with all this variance in your variant options...
Pooled Library Amplifications

Type

Blog Post

...hundreds of nanograms of DNA, you will likely need to perform several individual transformations, such as splitting...
Antibodies 101: Flow Compensation

Type

Blog Post

...corrected flow plot in 2A, the populations are not perfectly straight, but the center of each population is...
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Type

Blog Post

...PMCID: PMC4777285. Further Reading Microscopy U. Generfal Considerations when Using Fluorescent Proteins...
Fluorescent Proteins 101: Fluorescent Protein Timers

Type

Blog Post

...isolate particular organelles and their contents, or perform in situ hybridization experiments. In many cases...
Addgene's Cancer Collection Pages Connect the Research Community

Type

Blog Post

...growth and proliferation, while tumor suppressors perform the opposite task, keeping cells from dividing ...
Light Sheet Fluorescence Microscopy

Type

Blog Post

...scanning microscopy . Since image acquisition is performed using cameras with electron multiplying couple-charged...
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Type

Blog Post

...starving. I think plant biology is massively underfunded considering how important it is. I would really...
Which Fluorescent Protein Should I Use?

Type

Blog Post

...it is translated to a few hours. For example, superfolder GFP (sfGFP) and mNeonGFP can fold in <10min at...
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Type

Blog Post

...should be free to use and used for good. It's wonderful to have Addgene as a professional public-benefit...
Getting to Know Expansion Microscopy

Type

Blog Post

...can achieve. This means that regardless of how powerful of an objective you have, you can’t resolve two...
Great Results Start with Great Standard Curves

Type

Blog Post

...trendline. R2 ranges from 0 to 1 with 1 being a perfect fit and 0 indicating that there is no linear relationship...
SciComm: Taking Science to Elementary and Middle Schools

Type

Blog Post

...students each, and each group went on its own wonderful tangents. Some groups asked about vaccines, others...
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Type

Blog Post

... PCR/sequencing While CRISPR-transposons are powerful and simple to use, users should keep aware of ...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

Type

Blog Post

...of your PIPs in which the protein-interaction interface has been compromised (Kodama and Hu, 2012). Such...
CRISPR 101: Making a Knock-In Cell Line

Type

Blog Post

...increase HDR frequency by committing a break to performing HR before other factors were loaded onto the ...
Uncovering Ancestral Bias in CRISPR Research

Type

Blog Post

... screens they analyzed were designed to have a perfect match to the reference genome – even single mismatches...
Deep Dive: Fixing and Permeabilizing for Immunofluorescence

Type

Blog Post

..., Diaz MFP, Yong WH (2018) An Introduction to Performing Immunofluorescence Staining. Methods in Molecular...
AAVs in Retinal Gene Therapy

Type

Blog Post

... the cell’s nucleus. But since nothing’s ever perfect, neither is AAV. Its main limitation is the small...

Showing: 541 - 560 of 632 results

Results per page:

Tips for a 1st time AAV user (by a Rookie AAV user)

PCR Applications for Copy Number Variation Assays

Pooled Library Amplifications

Antibodies 101: Flow Compensation

Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

Fluorescent Proteins 101: Fluorescent Protein Timers

Addgene's Cancer Collection Pages Connect the Research Community

Light Sheet Fluorescence Microscopy

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Which Fluorescent Protein Should I Use?

Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Getting to Know Expansion Microscopy

Great Results Start with Great Standard Curves

SciComm: Taking Science to Elementary and Middle Schools

INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

CRISPR 101: Making a Knock-In Cell Line

Uncovering Ancestral Bias in CRISPR Research

Deep Dive: Fixing and Permeabilizing for Immunofluorescence

AAVs in Retinal Gene Therapy

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