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We narrowed to 929 results for: INA

Showing: 541 - 560 of 929 results

  1. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...Cas9 activators available in lentiviral format. Finally, while we can’t currently produce custom gRNA-containing...representation in the viral prep, infect target cells, and, finally, conduct the screen. To help alleviate some of...any questions or comments to [email protected]. Finally we’d like to thank all those who are allowing us...

  2. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ..., cyan, or magenta) depending on the hairpin combination. A gRNA containing all three hairpins produces...generates blue, green, or red colors for labeling. A combination of MS2, PP7, or boxB sequences in the same sgRNA...increase stability and minimize misfolding and recombination during virus production needed to deliver CRISPR-Sirius...

  3. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ... credit for small contributions. We strive to eliminate these two barriers, creating a community where...other scientists and to help in Crowdfight’s coordination work.  We also encourage you to file a request...Biomédica de Salamanca (IBSAL) and one of the coordinators at Crowdfight. She is currently based in Spain...

  4. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...these materials may not be available from their original sources. Figure 1: Biological repositories...availability of this strain from The Jackson Laboratory. Originally developed and characterized by Stanley Perlman...materials to be deposited into repositories, and financial support to maintain repositories’ capacity for...

  5. Hot Plasmids - January 2023

    Type
    Blog Post
    ...Generated against full length GFP and converted to recombinant form by the NeuroMab Facility Isotype IgG2a ...a polyclonal, with the reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies...  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...

  7. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...community-editable resource for storage and dissemination of information about FPs. FPbase.org FPbase...data to existing proteins when missing, or edit inaccuracies as they find them (anyone can register with ...community-editable database is free from the risk of inaccurate data submissions, so FPbase includes a versioning...

  8. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...DNA preps with multiple samples without cross contamination. We currently use the CosMCPrep system for this...new NEBNext Ultra II FS DNA Library Prep Kit for Illumina sequencing and took a class at NEB’s new teaching...prior NEBNext Ultra II DNA Library Prep Kit for Illumina, one was only able to extract libraries from previously...

  9. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...to break membranes of different organisms. And finally, we demonstrated that R bodies can be “tuned” with...F. R. Organization and expression of genetic determinants for synthesis and assembly of type 51 R bodies...trait: Absence of toxicity of R-body producing recombinant E. coli on paramecia. European Journal of Protistology...

  10. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ... by geneticists to study gene function. In this final installment, I’ll provide a brief introduction as...transgenes in different loci allows for genetic recombination so that the transgenes can be inherited on the...Engineering Posts Use FLEx Vectors for Site-Specific Recombination Resources at Addgene.org Browse Drosophila...

  11. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...since it is impossible to check all possible combinations of biology and equipment. Because the performance...(Goedhart et al, 2011) allows for a precise determination of brightness in cells, under realistic experimental...fluorescent protein in the host cell, which is a key determinant of the practical brightness. Therefore, practical...

  12. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...s ability to process its own pre-crRNA arrays eliminates the need to include multiple promoters to drive...processing, i.e. tRNAs or Csy4 cleavage sites. This eliminates bulk from crRNA expression plasmids resulting...crRNA array (bolded red arrow).  This undoes the original reversing of the direct repeats, leading to expression...

  13. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...insert targeted mutations through homologous recombination. To further increase the utility of CRISPR/Cas9...containing the RNA pol III promoter, gRNA and pol III terminator. These relatively large cassettes (considering...Size is also a factor; the pol lll promoter and terminator need be encoded only once, and each <200 bp tRNA-gRNA...

  15. Hot Plasmids - May 2022

    Type
    Blog Post
    ...method (M-CREATE) by: Brian O'Neill  The Viviana Gradinaru Lab has previously developed the multiplexed version...mutations. These plasmids contain catalytically inactive dCas9 and microbial SSAPs (dCas9-SSAP) which insert...specific transcription response element (TRE) or kinase translocation reporters (KTRs). Find visual barcode...

  16. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...Did you catch our April AAV webinar with Tim Miles, PhD, Director of the CLOVER Center at CalTech? If...are sold in packages of 10…)  If you missed the webinar, you can watch the presentation and Q&A session...recent review.      And that's a wrap on our AAV webinar! Special thanks to our panel of experts, Tim Miles...

  17. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...support the community in developing better, more sustainable, easily sharable products. Open-Source Microscopy...makerspace networks and training users via workshops, seminars, or hackathons greatly increases accessibility...brings stronger networking across laboratory and disciplinary boundaries and can allow for innovative organizations...

  18. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...log off for the day. 1:45 PM--Write final report. Work on a final report for a user research project I...help implement solutions for the stakeholders. Finally, software product managers test the solutions with...

  19. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ... approach, and conclusions (see above). Keep original files in a separate folder: You will often use...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...that was temporarily used. Just be sure that the original code remains in tact! Take notes Comment in ...

  20. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...questions the conclusion of inter-homologue recombination was released. This blog has not been updated...al. were interested in correcting an autosomal dominant mutation in MYBPC3 that leads to hypertrophic ...embryos to find those that do not carry a given dominant disease-causing gene, or that carry only one copy...
Showing: 541 - 560 of 929 results