We narrowed to 1,085 results for: IND

...site selection by TnsD, a sequence-specific DNA binding protein. Selection removes donor bacteria and recipient...subtilis to the bacterium of interest following IPTG induction of conjugation genes and bi-parental mating. Following...and colleagues used Mobile-CRISPRi to knockdown individual genes in several bacteria associated with human...measured after 6 and 12 generations with or without induction of CRISPRi knockdown. As expected, bacteria expressing...move Mobile-CRISPRi into your bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References...Addgene.org Browse our CRISPR interference plasmids Find bacterial CRISPR plasmids Read our CRISPR guide ...

...this kind of animal models. Start using adenoviral vectors with your CRISPR/Cas9 research! To find more...cells, Cas9 machinery is transiently expressed and binds to target DNA. In the final step of targeted gene...region. The CRISPR/Cas9-derived RNA-guided nuclease-induced gene disruption frequencies in the various cell...looking for a broader range of CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and...delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human...

... and an extension sequence containing a primer binding site and a reverse transcription template (RTT)...high-throughput libraries My labmates and I wanted to find a way to predict the efficiencies of pegRNA designs...the RTT overhang, the GC content of the primer binding site, and many more. Once we had our datasets with...experiments Prediction models only become useful once they find their way into the hands of users. For this reason...resources References Akhtar, W., De Jong, J., Pindyurin, A. V., Pagie, L., Meuleman, W., De Ridder, J....B. (2021). Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance....

... switch to Golden Gate Cloning easier because individual labs will not need to ‘domesticate’ all the basic... they were developed mostly with model plants in mind, Arabidopsis and Nicotiana benthamiana. N. benthamiana...Golden Gate cloning system especially with plants in mind. When it’s ready, I’ll make those available on my... up making 30 constructs. They’ve never done any kind of cloning and they don’t have any prejudice about... active on Twitter. Sometimes it’s a good way to find out what’s happening in the office next to you. ...remarkable number of female scientists whose tweets I find really inspirational. They share experiences, articles...

... determines how long and precise we can track individual molecules. Poor photon budgets are a widespread... synthetic fluorophore-contianing “ligand” that binds to the tag. Like most good ideas in biological imaging...proteins (FAPs) – These modified antibody fragments bind and enhance small molecule fluorogens (Figure 1e... encoded tag is required as the small-molecule binding motif targets endogenous protein (Figure 1f). ...when free in solution, but high fluorescence upon binding to its cognate protein. In some cases this property...Request Plasmids from the Michael Davidson Collection Find Fluorescent Protein Kits ...

...protein (FP) is split into two fragments that individually lack fluorescence. When complementary FP fragments...resources in less genetically tractable organisms lags behind. BiFC can also be used to screen for small-molecule...carry out because it relies on a single BiFC pair. Find BiFC plasmids for your experiment at Addgene! How... the random association between FP fragments independent of PPIs. Overexpression can be assessed by comparing...fluorescence due to the random association between FPs independent of PPIs. Simply compare the fluorescence intensity... introduction to FRET Resources on Addgene.org Find fluorescent protein plasmids and resources Visit...

...of genome engineering via CRISPR.  Though the individual topics are not surprising, the real value here...Science,” he describes the now infamous tale of Rosalind Franklin and her essential-but-unlauded role in...quieter, more reserved Franklins and Prashers will find it increasingly difficult to compete (for funding... quite a walk down memory lane for me. A brief window in time “The most obvious future consequence of... but that’s unlikely to be foremost in readers’ minds. Six months ago I would have nodded my head knowingly...book left them wanting to learn more. There are individual paragraphs that may have warranted their own ...

...proficiency.  What kind of content are you sharing?  Sometimes your subject matter will drive the kinds of communications... I want to put to rest a common misconception. I find personal bias often unintentionally plays a big ...My dad, on the other hand, mastered the art of finding how-to videos on YouTube long before he learned...audience already is.  You’ll also want to keep in mind that access and availability to resources (including...embark on a project, do your best to understand what kind of resources - including your time - will be required...

...expressing a protein that directly binds to zeocin, preventing it from binding DNA. Zeocin is an all-in-one ...a way to produce more of the plasmid, isolate individual plasmids, or express a gene contained in a plasmid...member of the bleomycin family of antibiotics, which induce cell death by intercalating into DNA and generating...genotoxic when not 100% inhibited, meaning it will induce mutations in the host/plasmid DNA during selection...the Sh ble gene does not fully suppress Zeocin-induced DNA cleavage in human cells. Mutagenesis, 20(2)...

...forum discussions about a wide variety of topics. Individual forums are hosted on subreddits (r/”topic”) which...yourself and your work out to other scientists who will find it useful, I highly recommend doing an AMA! Before...something we are, not something we do. We have to find a way to keep doing science to be happy. The happy... I said in the AMA, it’s incredibly important to find good mentors and incentivize good mentorship: AMA...get to speak publically about your work (you can find my tips for a perfect science talk here). Finally...the features I need to save, organize and easily find the resources that I come across on the internet...

...Use the links below to find Gateway plasmids for your organisms of interest: Find Gateway cloning plasmids...carry different antibiotic resistance markers (indicated here by plasmid color), allowing you to easily...pLenti CMV Puro DEST (w118-1) or the doxycycline-inducible pLIX_403. The chosen attR destination vector will...fragments, cDNA or Genomic DNA and is available for all kind of organisms from mammals to E. coli. Glossary of...Quickly Verify Your Clones Resources on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic Restriction...

...Plant Plasmids and Resources page Find synthetic biology plant plasmids Find CRISPR plant plasmids ...plant. A common method for silencing genes Virus induced gene silencing (VIGS) is a transient technique ...subcellular localization of your protein of interest. Find all plasmids from the Lee-Parsons lab. Test different...PMC6585625. Unver, Turgay, and Hikmet Budak. "Virus-induced gene silencing, a post transcriptional gene silencing...Addgene blog Read all of our plant biology blog posts Find tips for Arabidopsis transformation Read our Plasmids...

... above showed successful target modification, indicating that AAV is a good delivery vehicle for Cas9....cleavage activity in mammalian cells. SaCas9 produced indels at a similar efficiency to SpCas9, leading the ...tested two genes in mice. For both genes, they saw indel formation and phenotypic changes as early as 1 week...packaged in AAV.   Split AAV approaches  While finding smaller Cas9 orthologs works for some applications... in two pieces and package each piece into an individual AAV. When a cell is transduced by both AAVs, ...Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids ...

... To create the eGFP reporters the Harris lab individually mutated three codons in eGFP to eliminate fluorescence...capability of the seven human APOBEC3 proteins, finding that APOBEC3A and APOBEC3bctd are the most efficient...using a base-editor (C>T conversion) they could induce the translation of any detectable protein, not ...contained the cytidine base editor exhibited robust induction of GFP fluorescence confirming the efficacy of... universal, the Dow lab successfully used GO to induce the translation of an array of different reporters...Resources on Addgene.org Browse the CRISPR guide Find CRISPR plasmids for your research ...

...et al. 2010), inducible promoters with different degrees of stringency and inducibility (Toh et al. 2023... replication initiation factors and proteins independent of the host. Host range depends on whether the... vary in DnaA-box and iteron structure, host-independent   Building broad host range recombinant...2023), and additional cloning sites (Keen 1988).  Find broad host range recombinant vectors at Addgene!... (2023). Development of constitutive and IPTG-inducible integron promoter-based expression systems for...

...different or novel tools and assumptions to reveal findings that the originator was oblivious to, no matter...of the experiment would require access to each individual video. To PLOS’s credit, the organization subsequently...recognize that storing data in a sharable form is indeed a significant effort. Our standards of data recording...recording are highly variable and usually left to individual researchers. Yet accurate and comprehensible ...appears to leave the scope of data to be shared to individual authors. Minimally, raw but sufficiently annotated...charges of elitism and for scientists to be hiding behind our jargon and esoterica. Public trust in and funding...

...from the termite hindgut. Want to level up your or your class’s streak plate skills? Find the chromoprotein...Resources on Addgene.org Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids at ...the students to separate out colonies from each individual strain using the streak plate technique. Time...

...homology-directed repair (HDR) of a CRISPR/Cas9 induced double-stranded break. This site-directed genome...rendering the resulting construct resistant to Cas9 induced cleavage. Summary of Site Directed Mutagenesis ...is removed by DpnI restriction digestion leaving behind only the mutagenized plasmid. Although it is commonly...reaction. Ideally, your primers should be free of palindromic and repetitive sequences, but if present, a minor...similar approach can be used to identify Cas9/CRISPR-induced genome modifications. Occasionally, multimerization...Addgene.org Browse Our Molecular Biology Protocols Find Empty Vectors for Your Next Cloning Experiment ...

...on Addgene.org Find Synthetic Biology Plasmids Find Bacterial Expression Systems Find Plasmids for Protein...optimized for parameters such as induction temperature and length of induction, which will vary between different...gels containing Phos-Tag, a small molecule that binds specifically to phosphate moieties and promotes ...

...copies of the transgene that will recombine independently, cells may acquire one of a variety of colors...extremely versatile technology as recombination can be induced in developing fish via Cre protein microinjection...microinjection or crossing of Zebrabow animals to an inducible Cre line. The Schier group developed Zebrabow as ...powerful tool for understanding cell proliferation. Inducible XFP expression in the Ubow mouse Another murine...CFP, and YFP. After crossing onto a tamoxifen-inducible ubiquitin background, each cell in the mouse that...Köhler, Corinna, et al. "Mouse cutaneous melanoma induced by mutant BRaf arises from expansion and dedifferentiation...