We narrowed to 912 results for: IRE;

...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...either briefly boiling the sample before use or by directly adding a small volume of the sample to the PCR...tell you whether the insert has the correct directionality. Verifying the insert sequence with Sanger ...

...from long written proposal narratives. Now they require grant seekers to use online applications with significant...to and proof of compliance with the grantor’s requirements as well as state and federal regulations. A ... worked for an organization in a position that required some involvement in the grants process and managing...

... sites. In cases like these, antibodies can be directly conjugated to agarose or magnetic beads with commercially... commercially available chemical agents. Directly conjugating the primary antibody has the additional ... particularly advantageous because they do not require centrifugation between washes which can cause loss...

...on pEcgRNA plasmid as only two 24 nt oligos are required and the BsaI linearized pEcgRNA backbone can be... in large amounts and frozen for future use or directly ligated with the annealed 24 nt oligos to generate...the above systems are great for experiments that require only single chromosomal integration (ex: genome...

...generating reliable results from CRISPR edit data requires the help of robust software tools. As a consequence...of the edited sample. You can also download the entire analysis as a ZIP file by clicking the Download...the inferred distribution of indel sizes in the entire edited population of genomes. Hovering over each...

...the libraries for you.  Starting from plasmids requires amplification, verification, and lentiviral production...efficiency can vary across cell types and generally requires optimization of many variables, such as seeding...Using pooled libraries can be intimidating and require a large amount of experimental work, but with this...

...exposed even one time. Doctors and researchers don’t entirely understand why some people develop the disease...one of the best ways to improve the traditionally dire prognosis for mesothelioma patients is to catch ...inhibitor) prevents the growth of new blood vessels required to sustain growing tumors. Studies have shown ...

... following injection into the bloodstream, but requires a high vector dose and is unable to reach all ...The Deverman group then looked to see if PHP.B required the same receptors that its parental AAV9 relied...that PHP.B, unlike its parental AAV9, does not require galactose and AAVR to transduce cells. The Wilson...

.... Cre-dependent expression of the SPH activator paired with AAV sgRNA delivery make the SPH mouse an attractive...neuroscientists, not all neuroscience applications require such optimized tools. This is elegantly demonstrated...., 2018). The pooled nature of this experiment requires fluorescence activated cell sorting (FACS) to ...

...recombinase recognizes FRT sites and initiates site directed gene recombination between those sites. FRT sites...with low concentrations of vectors expressing the required recombinase. The high concentration of FLEx vectors...PMC41457. 8. Schnütgen, Frank et al. 2003. “A Directional Strategy for Monitoring Cre-Mediated Recombination...

...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything..., work-life negotiation, and mentorship. We can direct people to the AMA in the future and link to it ...an organizer/promoter was that I didn’t have the direct link to send people until the day of the AMA. This...

...all the steps and technical considerations are directly applicable to the rat [6]. Technical differences...Principles for applying optogenetic tools derived from direct comparative analysis of microbial opsins." Nature...Central PMCID: PMC3082132. 9.Del Bene, Filippo, and Claire Wyart. "Optogenetics: a new enlightenment age for...

...which is ideal for observing and manipulating the entire developmental cycle. A majority of studies involving...most significant advances is the sequencing of the entire axolotl genome, revealing an abundance of genes...year 2000, becoming the first plant to have its entire genome sequenced (Koornneff and Meinke, 2010). ...

... developed using yeast display technology, are directed to integrins that bind the Arginine-Glycine-Aspartate...2022). By contrast, LVDP-binding integrin α4β1 required and conferred less force, leading cells to spread...integrins, αVβ3 and αVβ5, participated but were not required for cell adhesion. The results surprised the investigators...

... the activity of the original, generated using directed evolution (Tei et al., 2023).  PLD can hydrolyze... doesn’t matter. Plus, for folks interested in directed-evolution methods, the activity-based selection... J. C., & Baskin, J. M. (2023). Activity-based directed evolution of a membrane editor in mammalian cells...

...really are selling yourself. A sales pitch that requires reading two pages or even a paragraph to get to... Simply stated, what are your aims? You could directly state your official aims, but I suggest distilling... and Structural Biology, and now serves as the Director of Student and Postdoctoral Programs at the Janelia...

...speaking early and often. Two of my jobs have indirectly resulted from someone seeing me speak in a non-interview... research, “aha” moments, and future research directions. Don’t worry if your work hasn’t been commercialized...does follow the general structure and flow of the entire session. I myself have a terrible habit of saying...

...Brainbow-1.0 in that transient Cre recombination is required to prevent constant inversion of the construct...derivatives of the fluorescent proteins, which are directly trafficked to cell membranes. Membrane trafficking... Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire E, Morin X, Livet J. Neuron. 2014 Feb 5;81(3)...

...advantage to using direct epigenetic modifiers? As with any experiment, your desired outcome will determine... DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety of ...

...guide and the transposon RNA homology both help direct the fusion construct to the new genomic locus. ...indel formation than methods relying on homology-directed repair (HDR), and insertion is scarless, unlike...where those interactions occur in the genome. This requires both targeting a specific genome location and ...