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We narrowed to 1,039 results for: LAS

Showing: 541 - 560 of 1039 results
  1. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ... team led by Kiran Musunuru and Rebecca Ahrens-Nicklas administered the first dose of his personalized... designated NGC-ABE8e-V106W. The name is hardly flashy, but that mouthful reflects the number of different..., Bick, S., Indurthi, V., Sharma, S., … Ahrens-Nicklas, R. C. (2025). Patient-Specific In Vivo Gene Editing...Clinic Resources on Addgene.org Browse CRISPR plasmids for base editing Read our CRISPR 101 eBook Brush...
  2. Evolution of Lab Techniques

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    Blog Post
    ...techniques comes in handy whether you are perusing classic papers or are brainstorming new technological innovations...blotting, restriction mapping and sequencing gels are classic techniques that allowed for many seminal scientific... on Analyzing DNA Sequencing Results Check out Plasmid Cloning by PCR Resources on Addgene.org Check...Pages Find Molecular Biology Tools Analyze Your Plasmids by Restriction Digest ...
  3. Finding Your Perfect Job After University

    Type
    Blog Post
    ...finishing my MSc. The masters program was 6 months of classes and 6 months of hands-on lab work for a dissertation...would like to do. I had taken advanced virology classes in California and enjoyed them, so I applied for...found out the job was with Addgene. I had used plasmids from this company before and recognised the logo...experiments over the phone or explain how various plasmids work. Most people who call or email me are scientists...
  4. A Guide to Getting Started in Undergrad Research

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    Blog Post
    ...Einstein and Bill Nye in their lab coats handling glassware with brightly colored hazardous liquids. Working...Sometimes teaches graduate and/or undergraduate classes Postdoctoral researcher (postdoc) Getting...responsibilities can vary "Lab assistants" usually wash glassware, prep media, etc. "Research assistants" are more...Feeling Like an Imposter: The Effect of Perceived Classroom Competition on the Daily Psychological Experiences...
  5. With an Eye Towards the Future, We Look Back at the March for Science

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    Blog Post
    ...Albany, NY, USA 42.6526°N 73.7562°W – Nicholas Thompson Over the last few years, there's been a disturbing...great, positive event that I hope happens again. Nicholas Thompson is a PhD Candidate studying Nuclear Science...Golden Hi, my name is Erin and I have spent the last 15 years training as an academic scientist. I’ve...
  6. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post
    ...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged...acid) that reacts with biarsenical dyes such as FlAsH (green, based on fluorescein) or ReAsH (red, based... Tetracysteine (Cys4) 15 Biarsenical dyes (FlAsH, ReAsH) Few Easy SNAP-tag 182 Benzylguanine...Addgene’s collection, so you may be able to get a plasmid ready-to-use without any cloning at all! As mentioned...
  7. Participate in the Pets of Addgene Scavenger Hunt

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    Blog Post
    ...blog.  We’ve been mostly working remotely for the last year to give our lab and shipping teams enough space...made their way onto our Slack workspace over the last year. Now, we’ve collected photos of our pets with... You will find five photos this way. At the very last photo, you will find a link to a form where if you...
  8. We’re Going Viral This Week!

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    Blog Post
    ...going viral,” we also have some other exciting news. Last week, we launched our new AAV Data Hub, an open ...production and data analysis. Our first paper came out last year on sucrose supplementation to increase AAV2...AAV2 yields and we’re eager to share more! Lastly, we’ll be at SfN2019. If you’re attending SfN2019, stop...
  9. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...How to Ligate Plasmid DNA... Protocols DNA Ligation DNA Ligation You may also like... Plasmid Cloning by Restriction...Restriction Enzyme Digest Restriction Digest of Plasmid DNA Bacterial Transformation Background Information...final step in the construction of a recombinant plasmid is connecting the insert DNA (gene or fragment ...physically attached to the backbone and the complete plasmid can be transformed into bacterial cells for propagation... and verifies the antibiotic resistance of the plasmid Cut vector - Background due to uncut vector Cut... Any colonies indicate contamination of intact plasmid in ligation or transformation reagents Optimizing...
  10. Congrats to the 2019 Deck The Lab Winners

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    Blog Post
    ...unseasonably warm weather we had last weekend). We’ve spent the last week perusing the Deck The Lab contest...2019 Hope you’ve enjoyed the festivities in the last month!  ...
  11. Twelve Amazing Guest Blog Contributors!

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    Blog Post
    ...and writing of two blog posts. Plasmids 101: Modular Cloning and Plasmids 101: Modular Cloning Applications...career advice collection. Embracing Serendipity Nicolas Mathis, who shared information on his open software...
  12. CRISPR Cheat Sheet

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    Blog Post
    ...Originally published May 31, 2018 and last updated Jan 27, 2021 by Jennifer Tsang. We have some crisp...commonly used CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease... to deliver. Not all cells efficiently take up plasmids used to produce CRISPR systems    ...
  13. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

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    Blog Post
    ...behalf.  If an individual obtains a cap subject H-1B (last year less than 30% of applicants received sponsorship...Please do not include the fact that you are a classically trained ballerina who has performed at Lincoln... papers, awards, presentations, in .pdf format.  Last but not least, NETWORK! Networking is extremely ...Resources on Addgene.org Careers at Addgene Global Plasmid Distribution ...
  14. Grad School Advice Part 2: Building Community

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    Blog Post
    ...Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your Research  ...Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring" We'd like to thank Niroshi and...
  15. How Do I Place an Addgene Order Using a PO Number?

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    Blog Post
    ...of information about how to deposit plasmids, store your plasmids or viral items, technical support, and...your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene,...
  16. Addgene's Website Update

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    Blog Post
    ...! You can use a manuscript title to search for plasmids from a specific paper. If you’re ready to start...everyone’s favorite section: Tools of the Day. Our plasmid and viral vector of the day have a fresh new look...new friend: Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day While those...
  17. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...about other Protein Tags in our Plasmids 101 Series. Get the Inntag Plasmids Tag endogenous proteins with... in a scientist’s toolbox. As we’ve covered in Plasmids 101, tags can help you determine localization ...
Showing: 541 - 560 of 1039 results