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We narrowed to 1,035 results for: LAS

Showing: 541 - 560 of 1035 results

  2. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    ...and writing of two blog posts. Plasmids 101: Modular Cloning and Plasmids 101: Modular Cloning Applications...career advice collection. Embracing Serendipity Nicolas Mathis, who shared information on his open software...

  3. CRISPR Cheat Sheet

    Type
    Blog Post
    ...Originally published May 31, 2018 and last updated Jan 27, 2021 by Jennifer Tsang. We have some crisp...commonly used CRISPR nuclease. Note: Most CRISPR plasmids from Addgene produce either a gRNA, a nuclease... to deliver. Not all cells efficiently take up plasmids used to produce CRISPR systems    ...

  4. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...behalf.  If an individual obtains a cap subject H-1B (last year less than 30% of applicants received sponsorship...Please do not include the fact that you are a classically trained ballerina who has performed at Lincoln... papers, awards, presentations, in .pdf format.  Last but not least, NETWORK! Networking is extremely ...Resources on Addgene.org Careers at Addgene Global Plasmid Distribution ...

  5. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...Resources on Addgene.org Browse Wu Lab Plasmids Find Plasmids for Your Research  ...Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring" We'd like to thank Niroshi and...

  6. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ...of information about how to deposit plasmids, store your plasmids or viral items, technical support, and...your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene,...

  7. Addgene's Website Update

    Type
    Blog Post
    ...! You can use a manuscript title to search for plasmids from a specific paper. If you’re ready to start...everyone’s favorite section: Tools of the Day. Our plasmid and viral vector of the day have a fresh new look...new friend: Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day While those...

  8. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...about other Protein Tags in our Plasmids 101 Series. Get the Inntag Plasmids Tag endogenous proteins with... in a scientist’s toolbox. As we’ve covered in Plasmids 101, tags can help you determine localization ...

  9. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ...circumstances – including unpredictable loud noises and flashing lights - that sent average, control mice into ...come to the right place. Get started with PGC-1 plasmids from Addgene. References: Skeletal muscle PGC....2014.09.012. PMID: 25295784 Related Materials: PGC-1a Plasmids      ...

  11. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...for material transfers. When Addgene entered the plasmid sharing space, we heard over and over again that...with the UBMTA result in quick approvals, so your plasmid requests can get to your lab faster. For Everybody...reference pages. So go ahead and use Addgene plasmids in your experiments. You know that they’re properly...

  12. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...Cas9 cleavage when using a photomasking technique. Last, Hemphill et al. presented data showing that this...S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek...Optogenetics and CRISPR: A New Relationship Built to Last. In: Methods in Molecular Biology. Springer US, ...Website Browse Our Collection of Optogenetics Plasmids Check out Our Genome Engineering Resources Brush...

  13. Deep Dive: qPCR

    Type
    Blog Post
    ... Though you’ll want to predict this value using BLAST, you’ll need to experimentally verify once you’ve...11237753. Primer design resources PrimerBlastDINAmelMfoldBLASTEnsembl More resources on the Addgene blog...blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...

  14. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...Table 1). The four systems Name Function Plasmid Transduction Marker Mouse Strain C-CHIME...  The X-CHIME deposit also includes pXPR_071, a plasmid that leads to higher titer lentivirus preparations...first generation CHIME (pXPR_053). Find X-CHIME plasmids here!   Marty LaFleur is a Postdoctoral Fellow...

  15. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...Plasmid pumpkins, team costumes, and spooky science stories. Yes, please! At Addgene, we love finding... contest trophy. We’ve had teams dressed up as plasmids, Blugene, and the Addgene ping pong table. This... of the algae cell is just the cell wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not...

  16. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...How to Ligate Plasmid DNA... Protocols DNA Ligation DNA Ligation You may also like... Plasmid Cloning by Restriction...Restriction Enzyme Digest Restriction Digest of Plasmid DNA Bacterial Transformation Background Information...final step in the construction of a recombinant plasmid is connecting the insert DNA (gene or fragment ...physically attached to the backbone and the complete plasmid can be transformed into bacterial cells for propagation... and verifies the antibiotic resistance of the plasmid Cut vector - Background due to uncut vector Cut... Any colonies indicate contamination of intact plasmid in ligation or transformation reagents Optimizing...

  17. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...Resources on Addgene.org Find Biosensor Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide...accounted for in your experimental design. Find nLight plasmids here! Next steps While nLightG and nLightR greatly...

  18. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    ...viral vectors (AAV), neuroscientists can combine classical genetic tools with the manipulation of specific...combinations to choose from for your experiments! Late last year, we hosted our first AAV Education and Development...of molecular tools and sensors Combinations of classical genetic tools, for example Cre/loxP or Flp/FRT...

  19. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...moved a gene required for phage propagation to a plasmid that lacked a promoter — only CAST integration ...integrate DNA payloads of up to 15 kb. Find evoCAST plasmids here! The new horizons of evoCAST In the emerging...Addgene's CRISPR Guide (Jump to Large Edits) CAST Plasmid Collection ...

  20. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...These receptors are specific to isotype class or even subclass, and are part of the reason why different...antibodies on a pathogen activates the immune system's classical complement pathway, starting a cascade of events...
Showing: 541 - 560 of 1035 results