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  1. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...requiring rapid maturation and prolonged imaging with minimal background.   Find these C. elegans mScarlet plasmids...18 kDa) and STReTCh (11 kDa) are significantly smaller than FRET-based mechanosensors (~60 kDa) and are...

  2. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...Yersinia enterocolitica, Xanthomonas campestris, X. maltophilia IncW pSa, pR388 Gram-negative  Acinetobacter...campestris pv. campestris, X. campestris pv. malvacearum, Zymomonas mobilis pBBR1 based pBBR1 ...

  3. Viral Production at Addgene

    Type
    Blog Post
    ...properties of the final product, particularly in large animal studies. Endotoxin contamination is minimized by...MRP 30 kDa subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma...

  4. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...://doi.org/10.1371/journal.pone.0158294 Chari R, Mali P, Moosburner M, Church GM (2015) Unraveling CRISPR-Cas9...Tsherniak A, Kadoch C (2018) Interrogation of Mammalian Protein Complex Structure, Function, and Membership...

  5. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ... the body, where it becomes embedded due to the small, hook-like shape of asbestos fibers. Over time these... HMGB1, the high mobility group box 1 protein. Normally localized to the nucleus, if the cell dies from...

  6. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...entirely. The other advantage is that these tags are small (8-12 amino acids) and neither disturb POI folding...toxic for the cells and affects the labeling of small structures like neurites. Like epitope tags, low...

  7. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...Magnetic beads are solid spheres and tend to be much smaller than agarose beads. Proteins conjugate on the surface...following the incubation step. The wash buffer has an optimal pH and ionic strength that breaks weak interactions...

  8. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ... are also great at infecting neurons and cause minimal inflammatory response, but are not taken up by ...References: Crawford KHD, Eguia R, Dingens AS, Loes AN, Malone KD, Wolf CR, Chu HY, Tortorici MA, Veesler D, Murphy...

  10. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... treat a bacterial infection with a lower than optimal drug concentration. Drug resistance is not a black...patient infection. Limiting the use of antibiotics in animal farming is already being recognized as an important...

  11. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ... a homogenous cell population in culture, it is normal to observe diversity in cell size, marker expression...s origin mainly stem from cell debris and other small fragments. Where exactly a given cell population...

  12. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... conformational complexity There are 24 known mammalian integrins, all heterodimers composed of one of...sequences that imitate this motif and compete with both small molecule integrin inhibitors and natural ligands...

  13. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... my expensive habits of running shoes and single malt whiskies, but I remember the days of modest postdoc... very hoppy pint of IPA or a few drams of single malt (preferably from Islay or Skye). Good luck with ...

  14. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... a crowd. Having answer slides ready after your formal conclusion slide will also help with your confidence...with other ways to engage the audience. Take an informal poll by asking for a show of hands on a particular...

  17. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ... which are commonly used to drive expression of small RNAs in their respective species. Addgene carries...allow researchers to insert targeting oligos with minimal cloning required. As with other model systems, ...

  19. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...of one AAV serotype with the capsid of another. Formally, pseudotypes are described with two numbers separated...using the genomic structure of AAV2, so they are formally called rAAV2/1, rAAV2/2, and so on, although we...

  20. A Control for All Seasons

    Type
    Blog Post
    ...antibody is replaced with pre-immune serum from the animal the antibody was produced in or an isotype-matched...primary antibody is incubated with its antigen or a small epitope-containing peptide to block the antigen-...
Showing: 541 - 560 of 811 results