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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 816 results for: MAL

Showing: 541 - 560 of 816 results

  1. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...can be used. The Fujii lab demonstrated this in mammalian cells by expressing dCas9 with an N-terminal 3xFLAG...expression of IGF2, a characteristic of many human malignancies. Additionally, the Tapscott Lab identified regulators...

  2. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...answer to the space problem. Instead of imaging smaller, what if we just made the sample bigger? In ExM...provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid...

  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...higher Tm give more efficient recombination. We normally use a Tm between 47-55 °C for the homologous region...be very helpful to confirm primer design.  Use minimal template DNA Use 1 ng of PCR template DNA to minimize...

  4. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...://doi.org/10.1371/journal.pone.0158294 Chari R, Mali P, Moosburner M, Church GM (2015) Unraveling CRISPR-Cas9...Tsherniak A, Kadoch C (2018) Interrogation of Mammalian Protein Complex Structure, Function, and Membership...

  5. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...requiring rapid maturation and prolonged imaging with minimal background.   Find these C. elegans mScarlet plasmids...18 kDa) and STReTCh (11 kDa) are significantly smaller than FRET-based mechanosensors (~60 kDa) and are...

  6. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...Yersinia enterocolitica, Xanthomonas campestris, X. maltophilia IncW pSa, pR388 Gram-negative  Acinetobacter...campestris pv. campestris, X. campestris pv. malvacearum, Zymomonas mobilis pBBR1 based pBBR1 ...

  7. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ... the body, where it becomes embedded due to the small, hook-like shape of asbestos fibers. Over time these... HMGB1, the high mobility group box 1 protein. Normally localized to the nucleus, if the cell dies from...

  8. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... treat a bacterial infection with a lower than optimal drug concentration. Drug resistance is not a black...patient infection. Limiting the use of antibiotics in animal farming is already being recognized as an important...

  9. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...Magnetic beads are solid spheres and tend to be much smaller than agarose beads. Proteins conjugate on the surface...following the incubation step. The wash buffer has an optimal pH and ionic strength that breaks weak interactions...

  10. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ... are also great at infecting neurons and cause minimal inflammatory response, but are not taken up by ...References: Crawford KHD, Eguia R, Dingens AS, Loes AN, Malone KD, Wolf CR, Chu HY, Tortorici MA, Veesler D, Murphy...

  11. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...entirely. The other advantage is that these tags are small (8-12 amino acids) and neither disturb POI folding...toxic for the cells and affects the labeling of small structures like neurites. Like epitope tags, low...

  13. Viral Production at Addgene

    Type
    Blog Post
    ...properties of the final product, particularly in large animal studies. Endotoxin contamination is minimized by...MRP 30 kDa subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma...

  15. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ... which are commonly used to drive expression of small RNAs in their respective species. Addgene carries...allow researchers to insert targeting oligos with minimal cloning required. As with other model systems, ...

  16. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... a crowd. Having answer slides ready after your formal conclusion slide will also help with your confidence...with other ways to engage the audience. Take an informal poll by asking for a show of hands on a particular...

  18. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ... a homogenous cell population in culture, it is normal to observe diversity in cell size, marker expression...s origin mainly stem from cell debris and other small fragments. Where exactly a given cell population...

  19. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... conformational complexity There are 24 known mammalian integrins, all heterodimers composed of one of...sequences that imitate this motif and compete with both small molecule integrin inhibitors and natural ligands...
Showing: 541 - 560 of 816 results