Skip to main content

We narrowed to 810 results for: Mal;

Showing: 541 - 560 of 810 results
  1. Viral Production at Addgene

    Type
    Blog Post
    ...properties of the final product, particularly in large animal studies. Endotoxin contamination is minimized by...MRP 30 kDa subunit (RPP30) as a control for normalization purposes are being used. Quality Control Mycoplasma...
  2. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...Magnetic beads are solid spheres and tend to be much smaller than agarose beads. Proteins conjugate on the surface...following the incubation step. The wash buffer has an optimal pH and ionic strength that breaks weak interactions...
  3. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ... are also great at infecting neurons and cause minimal inflammatory response, but are not taken up by ...References: Crawford KHD, Eguia R, Dingens AS, Loes AN, Malone KD, Wolf CR, Chu HY, Tortorici MA, Veesler D, Murphy...
  4. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ... the body, where it becomes embedded due to the small, hook-like shape of asbestos fibers. Over time these... HMGB1, the high mobility group box 1 protein. Normally localized to the nucleus, if the cell dies from...
  5. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... treat a bacterial infection with a lower than optimal drug concentration. Drug resistance is not a black...patient infection. Limiting the use of antibiotics in animal farming is already being recognized as an important...
  6. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...://doi.org/10.1371/journal.pone.0158294 Chari R, Mali P, Moosburner M, Church GM (2015) Unraveling CRISPR-Cas9...Tsherniak A, Kadoch C (2018) Interrogation of Mammalian Protein Complex Structure, Function, and Membership...
  7. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ... a homogenous cell population in culture, it is normal to observe diversity in cell size, marker expression...s origin mainly stem from cell debris and other small fragments. Where exactly a given cell population...
  8. Tips for Getting a Faculty Position

    Type
    Blog Post
    ... my expensive habits of running shoes and single malt whiskies, but I remember the days of modest postdoc... very hoppy pint of IPA or a few drams of single malt (preferably from Islay or Skye). Good luck with ...
  9. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... conformational complexity There are 24 known mammalian integrins, all heterodimers composed of one of...sequences that imitate this motif and compete with both small molecule integrin inhibitors and natural ligands...
  10. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ... which are commonly used to drive expression of small RNAs in their respective species. Addgene carries...allow researchers to insert targeting oligos with minimal cloning required. As with other model systems, ...
  11. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... a crowd. Having answer slides ready after your formal conclusion slide will also help with your confidence...with other ways to engage the audience. Take an informal poll by asking for a show of hands on a particular...
  12. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...biomolecular condensate in human cells — causes a rare malformation syndrome (Mensah & Niskanen et al., 2023). Now...oxygen consumption and upregulating other genes normally expressed in BAT. When donor-derived CRISPRa-AAV-treated...
  13. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...collaborative learning style at all. Finally I rotated in a small lab with a new PI. I realized that as a first born...collaboration? If you are considering a career at a small, teaching college you might want to consider less...
  14. A Control for All Seasons

    Type
    Blog Post
    ...antibody is replaced with pre-immune serum from the animal the antibody was produced in or an isotype-matched...primary antibody is incubated with its antigen or a small epitope-containing peptide to block the antigen-...
  15. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...of endogenous light-sensitive receptors, like mammalian rhodopsin, and the intracellular domain of an ...experiments.  Wavelengths Most vectors have an optimal wavelength to use but can be activated by a range...
Showing: 541 - 560 of 810 results