We narrowed to 876 results for: NSI;
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TypeBlog Post...perfect fit for Addgene. She was Head of Quality at Dimension Therapeutics, which was acquired by Ultragenyx... in 2017. As one of the original employees at Dimension Therapeutics, she helped build and grow the gene...
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Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...2019) Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of Packaged Single-Stranded Adeno-... A Rapid and Robust Assay for Batch-to-Batch Consistency Evaluation of AAV Preparations. Molecular Therapy... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog Post...interpretations. A good peer-reviewer will help spot inconsistencies in the work, missing information that would...non-native English speaker, I sometimes miss the sensitivity to judge how aggressive my sentences might sound... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...Although we were unable to deduce the mechanism responsible we still believe that these results will be beneficial... cores and laboratories alike as an easy and inexpensive method to improve AAV yields. Although we only... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post...important as its capabilities are the practical considerations of actually using it - the device has a rechargeable...expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the brain ... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...our early stage. After each pitch we made, we considered the feedback and adjusted our business model ...company) and is our money guy (CFO). As CEO, I am responsible for internal operations and building external... -
Another Pathway into Cells: iTOP
TypeBlog Post...iTOP is best suited to binary systems in which transient protein expression has a long-lasting effect, ...in the genome. Second, expression is by nature transient, which may lower nuclease toxicity or the potential... -
Extracurricular Activities for a Strong Science Career Path
TypeBlog Post...impact your work? What design variables need consideration? Enrich your knowledge and skills outside of...network Attend informational interviews to gain insight into the daily routine of the potential job Join... -
Molecular Biology Protocol - Restriction Digest of Plasmid DNA
TypeProtocol... check to make sure that it isn't methylation sensitive . Plasmids grown in Dam or Dcm methylase...opens in a new window) NEB's table of methylation sensitive restriction sites . Sometimes enzymes cut sequences...diagnostic digest), you can create a "Master Mix" consisting of all of the reaction components except for ...each tube. This will save you time and ensure consistency across the reactions.... -
25 Work From Home Tips for Scientists
TypeBlog Post... Everyone. Plan out new experiments carefully. Consider submitting a registered report, where peer review...is based on the methods, not the results. Also consider using this framework to design your future experiments... -
New and Upcoming Viral Vectors - September 2019
TypeBlog Post...for specific parameters: jGCaMP7s is the most sensitive responder to action potentials. For a single action... variant 1513-WPRE (105322-AAV1) GABA sensors Sensing levels of g-Amino butyric acid (GABA) - the primary... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...production, assembled AAV vector particles accumulate inside the producer cells and purification protocols often...the outside surface of the exosome or are carried inside the vesicle, which may also contain other cellular... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...manipulate neural populations in order to get new insights into functional network interactions. Combining...bacterial strain. The sensor will be transported inside a small 3D printed robot that will “swim” in the... -
Trading Spaces - Visiting Scientist Edition
TypeBlog Post...new institute and mentor that they may want to consider for future work placements. The chance to learn... expectations are reasonable, your reagents are onsite before you need them, and you are set up for success... -
K. phaffii: Rising to the Occasion
TypeBlog Post...model yeast strain and discuss why you may want to consider a yeast off the beaten path. Yeast as a model...for your media buck - it grows at higher cell densities than most of its counterparts, yielding higher... -
FPbase: A new community-editable fluorescent protein database
TypeBlog Post... Addgene blog – outlining various important considerations when choosing a FP, but much of the primary... (PostgreSQL), that aims to eventually be a comprehensive community resource for information about fluorescent... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...Although highly useful, this technique requires extensive sample processing to avoid disrupting ribosome-mRNA...protein levels were negatively correlated with GFP intensity, showing that TRICK is an accurate readout of ... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter... to make her work understandable to people both inside and outside the scientific community. We’re excited... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog Post... However, the pH changes in our solutions are transient and return to normal once the tube is opened and...restored. We have no evidence to suggest that the transient change in pH affects AAV function. Furthermore... -
Science Career Options
TypeBlog Post...computer programming skills? Other things to consider are wage, opportunity to advance, and how many...jobs are normally available (as sectors such as forensics currently have too many applicants compared to...