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Addgene

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Showing: 541 - 560 of 876 results
  1. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...perfect fit for Addgene. She was Head of Quality at Dimension Therapeutics, which was acquired by Ultragenyx... in 2017. As one of the original employees at Dimension Therapeutics, she helped build and grow the gene...
  2. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...important as its capabilities are the practical considerations of actually using it - the device has a rechargeable...expressing channelrhodopsin 2 (ChR2), a blue light sensitive cation channel, fused to mCherry to the brain ...
  3. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...our early stage.  After each pitch we made, we considered the feedback and adjusted our business model ...company) and is our money guy (CFO).  As CEO, I am responsible for internal operations and building external...
  4. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...iTOP is best suited to binary systems in which transient protein expression has a long-lasting effect, ...in the genome. Second, expression is by nature transient, which may lower nuclease toxicity or the potential...
  5. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ...impact your work? What design variables need consideration? Enrich your knowledge and skills outside of...network Attend informational interviews to gain insight into the daily routine of the potential job Join...
  6. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ... check to make sure that it isn't methylation sensitive . Plasmids grown in Dam or Dcm methylase...opens in a new window) NEB's table of methylation sensitive restriction sites . Sometimes enzymes cut sequences...diagnostic digest), you can create a "Master Mix" consisting of all of the reaction components except for ...each tube. This will save you time and ensure consistency across the reactions....
  7. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ... Everyone. Plan out new experiments carefully. Consider submitting a registered report, where peer review...is based on the methods, not the results. Also consider using this framework to design your future experiments...
  8. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...for specific parameters: jGCaMP7s is the most sensitive responder to action potentials. For a single action... variant 1513-WPRE (105322-AAV1) GABA sensors Sensing levels of g-Amino butyric acid (GABA) - the primary...
  9. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...production, assembled AAV vector particles accumulate inside the producer cells and purification protocols often...the outside surface of the exosome or are carried inside the vesicle, which may also contain other cellular...
  10. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...new institute and mentor that they may want to consider for future work placements. The chance to learn... expectations are reasonable, your reagents are onsite before you need them, and you are set up for success...
  11. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...model yeast strain and discuss why you may want to consider a yeast off the beaten path.   Yeast as a model...for your media buck - it grows at higher cell densities than most of its counterparts, yielding higher...
  12. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...Although highly useful, this technique requires extensive sample processing to avoid disrupting ribosome-mRNA...protein levels were negatively correlated with GFP intensity, showing that TRICK is an accurate readout of ...
  13. Science Career Options

    Type
    Blog Post
    ...computer programming skills? Other things to consider are wage, opportunity to advance, and how many...jobs are normally available (as sectors such as forensics currently have too many applicants compared to...
Showing: 541 - 560 of 876 results