dTAG - You're it!
Type
Blog Post
...proteins is an exciting strategy, it is difficult to apply this technique to oncoproteins that lack selective...
We narrowed to 663 results for: PPL;
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Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...developed by the James and Schuh labs. Clift et al. applied TRIM-away to nine proteins in ten ddifferent cell... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post...some reporters are better suited for particular applications. Read on to learn more about each reporter or... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...kinases good for? There are a number of potential applications for photoswitchable kinases. Below are a few... -
Transferable Skills Guide: Public Speaking
TypeBlog Post...comes to public speaking, Murphy’s law definitely applies: what can go wrong, will go wrong. The trash removal... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog Post...were developed before CRISPR-Cas9, for use in applications like siRNA and antisense oligonucleotides. Some... -
What's New in CRISPR - November 2021
TypeBlog Post...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease.... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...are particularly useful for certain research applications. Many secondary antibodies are polyclonal, as... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...increasingly been recognized for its biotechnological applications including conversion of CO2 into water and electrical... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...which can then be used for downstream cloning applications. While these two strains ultimately perform ... -
Addgene's Tips for Plasmid Quality Control
TypeBlog Post...growth instructions (temperature, strain, media supplements), suggested sequencing primers, or mammalian ... -
The Future of Research Symposium Boston 2015
TypeBlog Post...and from what fields – are needed to adequately supply these demands? Are there other solutions, such ... -
Plasmids 101: Cre-lox
TypeBlog Post...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ... -
Design Tips for Prime Editing
TypeBlog Post...its design changes for every new prime editing application. And optimizing its sequence is important, as... -
Making CRISPR Plasmids Using Fragmid
TypeBlog Post...validated and were chosen because of their broad applicability in different model systems. What’s the error... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post...retaining residual antibodies. If you have already applied antibodies and need to dry the membrane for storage... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...excitation or inhibition upon light (or drug) application. Genetic manipulation can also reduce expression... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: 21994751. PubMed... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...Proceedings of the National Academy of Sciences 100.suppl 1 (2003): 11861-11865. PubMed PMID: 12917490. PubMed... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...ura3 mutants and cannot grow without uracil supplementation (6). Expression of MET/Met genes from the pYES2.1...