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We narrowed to 921 results for: Por

Showing: 541 - 560 of 921 results

  1. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...transduced rat neurons in vivo with beta-gal and other reporters, and they showed that expression persisted for...Figure 2). The HIV genes that do remain are very important for viral production: Gag (structural precursor...activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral envelope protein...

  2. CRISPR Antimicrobials

    Type
    Blog Post
    ...species, or even an antibiotic resistance gene. Importantly, native, non-pathogenic bacteria remain to help...recolonize the niche, reducing the chance of an opportunistic infection like for organisms like C. difficile...colonization model, phagemid treatment decreased the proportion of virulent S. aureus from 50% to 11% in just...

  3. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...After sequencing and searching for cellular RNAs important for Cpf1 function, they found that mature crRNAs...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...

  4. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...recent lineage tracing approaches that led to important insights in developmental biology. Microscopy-...pathway regulated by one-eyed pinhead, incorrect proportions of otherwise normal cell types are produced, ...PMCID: PMC6247916. Felker, A., and C. Mosimann. "Contemporary zebrafish transgenesis with Tol2 and application...

  5. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    .... More importantly, the expression of this construct is Cre-dependent, enabling spatiotemporal control...astrocytes, and oligodendrocytes, provided that reporter genes specific to these cell types are used. The...

  6. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in the pool...activity of Cas13a becomes activated and the RNA reporter will be cleaved resulting in activation of the...in the field and as always, we appreciate the opportunity to share these invaluable reagents with the scientific...

  7. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...RNA editing - and further experiments strongly supported their initial findings.  The team originally suspected.... Specifically, efficacy rates of Cas7-11 were reported as up to 85%, broadly similar to shRNA and Cas13...However, in those same comparisons, the AbuGoot lab reported no detectable effect on cell viability – meaning...

  8. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...from 2016–2023 by type of tool. Equally as important to what is being delivered is where it’s being...vectors not only available, but accessible. The supporting data and community engagement required to do ...lessons learned from this process have shaped and supported our growth and development. We’ve shared what ...

  9. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...this post will, indeed, focus on bacteria, it is important to note that the term ‘antibiotic’ can also be...animal farming is already being recognized as an important step, as you can’t walk into any supermarket now...Tackling drug-resistant infections globally: final report and recommendations." London: Wellcome Trust & ...

  10. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...Labor Thomas Perez, Fair Pay for Postdocs: Why We Support New Federal Overtime Rules, made clear that institutional...National Postdoctoral Association Institutional Policy Report 2014). However, ~30% set their minima lower. Furthermore...consensus of academic stakeholders. There was ample opportunity to accept these recommendations, and make gradual...

  11. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...2016; Lambert, 2019). These characteristics are important, but have less priority than stickiness. In my...lead to over-expression artifacts. This is an important downside of the technology and should always be...fluorescent proteins in budding yeast." Scientific reports 9.1 (2019): 2234. PubMed PMID: 30783202. PubMed...

  12. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...split aptamer is composed of a “Top” and “Bottom” portion, each fused to a separate piece of RNA. Fluorescence...photostable, and were presented in the study to support the uniqueness of the photostability of the Corn-DFHO...growth as RNA biology, we are looking forward to reports on the next additions to aptamer soup! References...

  13. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...sequences, which facilitate integration of this portion into the host genome. The packaging plasmid contains...more detailed description). Since the integrated portion does not contain any of the viral packaging genes...the resulting double-stranded DNA transgene is incorporated into a large complex of both viral and cellular...

  14. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...signals. For this, you’ll need to gate based on the proportion between H and A (or W and A) within the same ... just as fine (Figure 4). As H and A scale in proportion (while H and W do not; see Figure 2), single ...                                                                                                         B.    Figure 4: (A) Using the proportional relationship between FSC-H and FSC-A we can ...

  15. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...in competition with other similar integrins, as reported by IPI and the Springer lab (Hao et al., 2024)...In this study, we, along with the Springer lab, reported the binding specificity, kinetics, and affinity...belief that αVβ3 would be the most functionally important integrin on these cells. What’s next IPI is working...

  16. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...invasion and D loop formation, the newly synthesized portion of the invasive strand is displaced from the template...DSB-induced genome engineering, it has biological importance for the repair of breaks that lack a second end...Cut-to-Mutation distance" affects editing efficiency. One important point to note is that Cas enzymes can continue...

  18. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...mycoplasma contamination The best and the most important ways to avoid mycoplasma are by providing proper...viability. Conclusion To sum up, it is always important to prevent contamination rather than eradicating...others when you can and help them understand the importance of preventing contamination. Be aware and cautious...

  19. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...of the gene with multiple gRNAs, but the most important is that you could avoid potential off target artifacts...you could run another gel that highlights the important results (for example, if you needed to make a ... Despite the shifting landscape of research opportunities, it’s an exciting time to be in the lab. Based...

  20. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...fibrillary acidic protein (GFAP) for astrocytes it is important to test it in the brain region of interest, especially...of Transduction Efficiency, Tropism and Axonal Transport of AAV Serotypes 1, 2, 5, 6, 8 and 9 in the Mouse... the Addgene blog Plasmids 101: FLEx Vectors Important Considerations When Using AAVs Three Key Considerations...
Showing: 541 - 560 of 921 results