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We narrowed to 928 results for: Por

Showing: 541 - 560 of 928 results

  1. CRISPR Antimicrobials

    Type
    Blog Post
    ...species, or even an antibiotic resistance gene. Importantly, native, non-pathogenic bacteria remain to help...recolonize the niche, reducing the chance of an opportunistic infection like for organisms like C. difficile...colonization model, phagemid treatment decreased the proportion of virulent S. aureus from 50% to 11% in just...

  3. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...dramatically when there are many new COVID-19 cases being reported (right, red), likely due to quarantining {%...chose to re-examine our global data at a finer temporal granularity. For each paper, we observed that ...parameters from relatively sparse data from China. An important additional caveat is that our dataset contains...

  4. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...by ddPCR begins with diluting the virus. It is important to note that the dynamic range of the ddPCR is...PCR plate. Maintaining a high droplet count is important for calculating the concentration of your sample...separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150 base pairs...

  5. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...After sequencing and searching for cellular RNAs important for Cpf1 function, they found that mature crRNAs...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...

  6. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...from 2016–2023 by type of tool. Equally as important to what is being delivered is where it’s being...vectors not only available, but accessible. The supporting data and community engagement required to do ...lessons learned from this process have shaped and supported our growth and development. We’ve shared what ...

  7. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...Labor Thomas Perez, Fair Pay for Postdocs: Why We Support New Federal Overtime Rules, made clear that institutional...National Postdoctoral Association Institutional Policy Report 2014). However, ~30% set their minima lower. Furthermore...consensus of academic stakeholders. There was ample opportunity to accept these recommendations, and make gradual...

  8. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...of interest and an inactivated fluorescent RNA reporter. If the target sequence is present in the pool...activity of Cas13a becomes activated and the RNA reporter will be cleaved resulting in activation of the...in the field and as always, we appreciate the opportunity to share these invaluable reagents with the scientific...

  9. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...this post will, indeed, focus on bacteria, it is important to note that the term ‘antibiotic’ can also be...animal farming is already being recognized as an important step, as you can’t walk into any supermarket now...Tackling drug-resistant infections globally: final report and recommendations." London: Wellcome Trust & ...

  10. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...RNA editing - and further experiments strongly supported their initial findings.  The team originally suspected.... Specifically, efficacy rates of Cas7-11 were reported as up to 85%, broadly similar to shRNA and Cas13...However, in those same comparisons, the AbuGoot lab reported no detectable effect on cell viability – meaning...

  11. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...recent lineage tracing approaches that led to important insights in developmental biology. Microscopy-...pathway regulated by one-eyed pinhead, incorrect proportions of otherwise normal cell types are produced, ...PMCID: PMC6247916. Felker, A., and C. Mosimann. "Contemporary zebrafish transgenesis with Tol2 and application...

  12. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    .... More importantly, the expression of this construct is Cre-dependent, enabling spatiotemporal control...astrocytes, and oligodendrocytes, provided that reporter genes specific to these cell types are used. The...

  13. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    ...evaluate the resulting expression of the fluorescent reporter, the brain tissue was extracted, sectioned, and...more neuroscience-focused tools in their Brain Map portal, and cell science-focused tools at the Allen Institute...expression from mouse to primate neocortex. Cell Reports, 34(13), 108754. https://doi.org/10.1016/j.celrep...

  14. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...sequences, which facilitate integration of this portion into the host genome. The packaging plasmid contains...more detailed description). Since the integrated portion does not contain any of the viral packaging genes...the resulting double-stranded DNA transgene is incorporated into a large complex of both viral and cellular...

  15. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...signals. For this, you’ll need to gate based on the proportion between H and A (or W and A) within the same ... just as fine (Figure 4). As H and A scale in proportion (while H and W do not; see Figure 2), single ...                                                                                                         B.    Figure 4: (A) Using the proportional relationship between FSC-H and FSC-A we can ...

  16. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...in competition with other similar integrins, as reported by IPI and the Springer lab (Hao et al., 2024)...In this study, we, along with the Springer lab, reported the binding specificity, kinetics, and affinity...belief that αVβ3 would be the most functionally important integrin on these cells. What’s next IPI is working...

  17. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...2016; Lambert, 2019). These characteristics are important, but have less priority than stickiness. In my...lead to over-expression artifacts. This is an important downside of the technology and should always be...fluorescent proteins in budding yeast." Scientific reports 9.1 (2019): 2234. PubMed PMID: 30783202. PubMed...

  18. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...split aptamer is composed of a “Top” and “Bottom” portion, each fused to a separate piece of RNA. Fluorescence...photostable, and were presented in the study to support the uniqueness of the photostability of the Corn-DFHO...growth as RNA biology, we are looking forward to reports on the next additions to aptamer soup! References...

  19. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...invasion and D loop formation, the newly synthesized portion of the invasive strand is displaced from the template...DSB-induced genome engineering, it has biological importance for the repair of breaks that lack a second end...Cut-to-Mutation distance" affects editing efficiency. One important point to note is that Cas enzymes can continue...
Showing: 541 - 560 of 928 results