We narrowed to 1,006 results for: RING
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TypeBlog Post...insertions were positive or negative selected for during the experiment (Sandoval-Villegas et al., 2021)...Targeting) system. INTEGRATE is based on a naturally occurring Tn7 transposon found in Vibrio cholerae that encodes...Sleeping Beauty, piggyBac and Tol2 for Genome Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084...about the INTEGRATE system for bacterial genome engineering Read about the Sleeping Beauty DNA transposon...
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Antibodies 101: Introduction to Antibodies
TypeBlog Post...letters and be able to guess whether or not that string of letters is a part of the word you’re searching...monoclonal antibodies, which come from naturally-occurring immunoglobulin genes, recombinant antibodies are...Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production of functional scFv antibody in E. ... the Addgene blog: Read about another reagent sharing platform for antibodies, the Developmental Studies... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...evidence,” and to speak out in unprecedented force during the 2015 federal election. Even as we marched... of the few members of Congress who holds an engineering degree. It was a great, positive event that I...a PhD Candidate studying Nuclear Science and Engineering at Rensselaer Polytechnic Institute. He also ...face of evidence. Stephanie Hays is moving from engineering synthetic microbial communities at Harvard University... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post... best known reversible PA-FP is the naturally occurring Dronpa protein (named after dron, a ninja term...dynamics (fission, fusion, etc). For example, by engineering PA-GFP tagged to a mitochondrial matrix protein...PMCID:PMC528746. 5.Gurskaya, Nadya G., et al. "Engineering of a monomeric green-to-red photoactivatable ...organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis." J Cell ... -
Lucky Thirteen: Guest Contributors of 2023
TypeBlog Post...Getting a Faculty Position Author: Erik Lee SnappSharing is Caring, But How to Distribute Open Hardware? Author... holiday celebrations! We're kicking it off by sharing all the incredible guest posts and stories featured... -
Enabling high school research at the Journal of Emerging Investigators
TypeBlog Post...breakdown Intro Chapter 1 - Introduction to Mark Springel, Olivia Ho-Shing, and the Journal of Emerging ...Many thanks to our guests,Olivia Ho-Shing, Mark Springel, Arooba Ahmed, Jiachen Lee, and Jillian Parker...to introduce students to this amazing genome enegineering technology Resources at Addgene.org Find basic... -
Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)
TypeBlog Post...a GIPHY page! The best part is, we’re not only sharing them with you, but you can share them with your...labmates, and friends as well! Addgene is sharing so much more than plasmids these days. via GIPHY...out everything we’ve uploaded so far, and start sharing the science love today! ... -
Imaging Tools and Following a Passion for Basic Biology: Interview with Joachim Goεdhart
TypeBlog Post...University of Amsterdam. In addition to creating and sharing many great fluorescent protein tools, Professor...and pay attention to the details. Chapter 3 - Answering interesting biological questions using microscopy... genome engineering... -
Depositor Week 2020 is Happening Now. Join us!
TypeBlog Post...prizes!), polls, and shout outs, and we’ll be featuring the work of the Addgene community. Perhaps you...Friday: Wrap up! To show the magnitude of plasmid sharing over the years, we crunched the numbers (shout ...news of deposits coming from two new countries bringing the total to 51 countries with plasmid deposits... -
Tips for Working in Industry & Success in Management: Interview with Crystal Shih
TypeBlog Post...completing her PhD in Chemistry and Chemical Engineering with Harry Gray at Caltech, Crystal moved on ...arise. Tips on applying for industry jobs and deciphering job listings. Mobility between different positions...Addgene Find more management resources in our Mentoring for Scientists Series Learn about Turning Your... -
Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service
TypeBlog Post... What if after ordering a plasmid you didn't have to grow the bacteria and prep the plasmid before you...preparative experiments. We’re excited to begin offering this service to further contribute to our mission... community and possibly expand the collection. During this period, we’d love to hear from you if you’... -
Newly Updated AAV Data Hub!
TypeBlog Post...and a key step for that is sharing the data when an AAVs doesn't work. Sharing your negative data helps ...others and, when published, will meet FAIR data-sharing guidelines. But what if my AAVs didn't work? ... -
Fueled by Coffee at #SfN14
TypeBlog Post... the neuroscience community pulls off such an inspiring, thought-provoking, collaborative event – all ... to help their labs save time and resources by storing and distributing their plasmids with the scientific...of this speaks to the importance of scientific sharing to the neuroscience community. The scientists we've... -
How Exercise Purges Stress and Keeps Depression at Bay
TypeBlog Post...control mice into depression. In case you were wondering, depression shows up in mice as poorer performance...kynurenic acid can’t cross the blood-brain barrier. By lowering circulating levels of kynurenine in the body, ...metabolites that are dynamically secreted by muscle during exercise to meet the brain’s physiological demands... -
Designer PUF Proteins for Any RNA Target
TypeBlog Post...has opened up a world of possibilities in the engineering of complex gene networks. A comparable system...assemble designer PUF domains for RNA specificity engineering. “The RNA binding domain is interesting because...report published in The Journal of Biological Engineering in March is fused to a translational repressor... -
Why Do I Need an MTA Anyway?
TypeBlog Post...legal complexities can arise when scientists are sharing their materials. Questions about intellectual property...material transfers. When Addgene entered the plasmid sharing space, we heard over and over again that the legal...were one of the biggest hurdles for scientific sharing. So we set out to streamline this process. We take... -
Stepping into a Virtual Internship: My Experience at Addgene
TypeBlog Post...figured this internship would be a perfect way of exploring my different interests but also a way of challenging...sciences, I knew I would run into some challenges during my internship, but nothing could have prepared ...challenges would be from doing a remote internship during a pandemic. Before I knew how bad COVID was getting... -
Scientist Networking: What is an Informational Interview?
TypeBlog Post...no secret that one of the best things you can do during your training is meet interesting people doing ...that position such as a direct connection to the hiring manager. They might say yes and they might say ...connections is your university’s alumni database. Sharing a school is a little connection that many people... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog Post...earlier, but the researchers in the directory are volunteering their time and resources and there is a chance...regulations from country to country when it comes to administering phage therapy. The laws behind phage therapy... open science Find blog posts about scientific sharing Find blog posts about networking Additional resources... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog Post...ago, Melina Fan had an idea and her family helped bring it to fruition. The concept, though relatively simple...was once a complicated process of requesting and sharing plasmids has become simplified through Addgene.... our impressive stats from 10 Years of Plasmid Sharing Or read more about what happens Inside Addgene ...