Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,009 results for: RING

Showing: 541 - 560 of 1009 results

  1. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...minimize recombination that are typically employed during viral plasmid amplification (Fischer, KB. 2019)... showing the mechanism of spontaneous reversion during AAV plasmid amplification. On the left homologous...  Some classes of neurons transiently express TH during development or have low levels of TH mRNA without...time, frustration, and funds in the long-run by ensuring you are minimizing any off-target expression that...

  2. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...insertions were positive or negative selected for during the experiment (Sandoval-Villegas et al., 2021)...Targeting) system. INTEGRATE is based on a naturally occurring Tn7 transposon found in Vibrio cholerae that encodes...Sleeping Beauty, piggyBac and Tol2 for Genome Engineering. IJMS 22:5084. https://doi.org/10.3390/ijms22105084...about the INTEGRATE system for bacterial genome engineering  Read about the Sleeping Beauty DNA transposon...

  3. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...the five is different. To identify significant pairings, additional tests, referred to as post-hoc analysis...temperature, humidity, sunlight exposure time, and watering volume on total crop production (though you would... error is the probability of a false positive occurring when many comparisons are performed. For example...drawbacks to these tests as they can be quite stringent if many samples are assessed. However, if many...

  4. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...questions in your own lab. 2. Seek multiple mentors during your postdoc At the end of grad school, you searched...own experience, great mentors often can be found during conversations with senior investigators. Discussions...fundamental conflict. Science is about exploration, discovering new information. There are literally infinite...well practiced or too skilled at presenting and answering questions. Organizing a thoughtful engaging talk...

  5. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...letters and be able to guess whether or not that string of letters is a part of the word you’re searching...monoclonal antibodies, which come from naturally-occurring immunoglobulin genes, recombinant antibodies are...Y, Srinivas S, Zhang Y, Lin M, Wang S (2013) Engineering production of functional scFv antibody in E. ... the Addgene blog: Read about another reagent sharing platform for antibodies, the Developmental Studies...

  6. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ... best known reversible PA-FP is the naturally occurring Dronpa protein (named after dron, a ninja term...dynamics (fission, fusion, etc). For example, by engineering PA-GFP tagged to a mitochondrial matrix protein...PMCID:PMC528746. 5.Gurskaya, Nadya G., et al. "Engineering of a monomeric green-to-red photoactivatable ...organelles shows that mitochondrial fusion is blocked during the Bax activation phase of apoptosis." J Cell ...

  7. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...evidence,” and to speak out in unprecedented force during the 2015 federal election.   Even as we marched... of the few members of Congress who holds an engineering degree. It was a great, positive event that I...a PhD Candidate studying Nuclear Science and Engineering at Rensselaer Polytechnic Institute. He also ...face of evidence. Stephanie Hays is moving from engineering synthetic microbial communities at Harvard University...

  8. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    ...Getting a Faculty Position Author: Erik Lee SnappSharing is Caring, But How to Distribute Open Hardware? Author... holiday celebrations! We're kicking it off by sharing all the incredible guest posts and stories featured...

  9. Enabling high school research at the Journal of Emerging Investigators

    Type
    Blog Post
    ...breakdown Intro Chapter 1 - Introduction to Mark Springel, Olivia Ho-Shing, and the Journal of Emerging ...Many thanks to our guests,Olivia Ho-Shing, Mark Springel, Arooba Ahmed, Jiachen Lee, and Jillian Parker...to introduce students to this amazing genome enegineering technology Resources at Addgene.org Find basic...

  12. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...prizes!), polls, and shout outs, and we’ll be featuring the work of the Addgene community. Perhaps you...Friday: Wrap up! To show the magnitude of plasmid sharing over the years, we crunched the numbers (shout ...news of deposits coming from two new countries bringing the total to 51 countries with plasmid deposits...

  13. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    ...completing her PhD in Chemistry and Chemical Engineering with Harry Gray at Caltech, Crystal moved on ...arise. Tips on applying for industry jobs and deciphering job listings. Mobility between different positions...Addgene Find more management resources in our Mentoring for Scientists Series Learn about Turning Your...

  14. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    ... What if after ordering a plasmid you didn't have to grow the bacteria and prep the plasmid before you...preparative experiments.  We’re excited to begin offering this service to further contribute to our mission... community and possibly expand the collection.  During this period, we’d love to hear from you if you’...

  15. Newly Updated AAV Data Hub!

    Type
    Blog Post
    ...and a key step for that is sharing the data when an AAVs doesn't work. Sharing your negative data helps ...others and, when published, will meet FAIR data-sharing guidelines. But what if my AAVs didn't work?  ...

  16. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ... the neuroscience community pulls off such an inspiring, thought-provoking, collaborative event – all ... to help their labs save time and resources by storing and distributing their plasmids with the scientific...of this speaks to the importance of scientific sharing to the neuroscience community. The scientists we've...

  17. How Exercise Purges Stress and Keeps Depression at Bay

    Type
    Blog Post
    ...control mice into depression. In case you were wondering, depression shows up in mice as poorer performance...kynurenic acid can’t cross the blood-brain barrier. By lowering circulating levels of kynurenine in the body, ...metabolites that are dynamically secreted by muscle during exercise to meet the brain’s physiological demands...

  18. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...has opened up a world of possibilities in the engineering of complex gene networks. A comparable system...assemble designer PUF domains for RNA specificity engineering. “The RNA binding domain is interesting because...report published in The Journal of Biological Engineering in March is fused to a translational repressor...

  19. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...legal complexities can arise when scientists are sharing their materials. Questions about intellectual property...material transfers. When Addgene entered the plasmid sharing space, we heard over and over again that the legal...were one of the biggest hurdles for scientific sharing. So we set out to streamline this process. We take...

  20. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    ...figured this internship would be a perfect way of exploring my different interests but also a way of challenging...sciences, I knew I would run into some challenges during my internship, but nothing could have prepared ...challenges would be from doing a remote internship during a pandemic. Before I knew how bad COVID was getting...
Showing: 541 - 560 of 1009 results