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We narrowed to 1,043 results for: STI;

Showing: 541 - 560 of 1043 results

  1. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...                           National Institute for Physiological Sciences Broad Institute                                       ...Hospital                           California Institute of Technology             Johns Hopkins University...Academy of Medical Sciences University of Texas at Austin University of California, San Francisco  University...

  2. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ... Twitter.” This paper addresses the following questions about scientists on Twitter: who are they? What...they are based on the US Occupational Employment Statistics (OES), but international Twitter users were included...orange)  scientists largely cluster by discipline, suggesting that scientists tend to follow scientists within...out the Top 50 Science Stars on Twitter Want suggestions on how to use social media as a scientist? Check...

  3. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    ... the panelists were interesting enough to make up for the somewhat naïve questions. During the panel, ...their seminal, cooperative paper in Science.  Interestingly, this project started with their labs studying...for billions of years (…) Bacteria are really sophisticated”.     New to CRISPR/Cas9 Technology? Learn ...

  4. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...14] Hepatocytes [15] Pancreatic β-cells [16] Intestinal tissue [17] Lung epithelial cells [18] Red ...approaches differ in several ways. Below is a table listing some of critical differences (adapted from Zhou...Zhou and Melton, 2008, [43]): Characteristic Reprogramming+ Transdifferentiation in vivo or in vitro...differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature, 2011. 470(7332): p. ...37. Merrell, A.J. and B.Z. Stanger, Adult cell plasticity in vivo: de-differentiation and transdifferentiation...

  5. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ... fastest way for us to release your plasmids is still for you to notify us as soon as your article is ...a project isn’t working out as planned, but you still hope somebody will find the plasmids useful, we’...article, a preprint, or without any paper at all. Posting preprints and openly sharing materials are both...think. We appreciate your feedback. If you have questions about depositing, check out our website, Help ...

  6. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...composed of camels, llamas, and alpacas (and their undomesticated counterparts) - not exactly species that jump... is a lot of excitement around using sdAbs in diagnostic applications. For example, fluorescently and ...generate new sdAbs. There has also been some interesting work done developing transgenic mice that express...S (2023) Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int J Mol Sci 24:5994. https...

  7. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...mitigating noise generated by off target digestion. After MNase digestion, fragments are released from nuclei... DNA around the target protein. This targeted digestion is controlled by the addition of calcium, which...measure of DNA binding sites in combination with existing ChIP-seq data sets, of which there are many readily...

  8. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...property by including ccdB on its destination plasmids. In the destination plasmid, the ccdB gene is flanked... interest by taking advantage of bacterial characteristics such as prototrophy, the ability to synthetize...sites. To introduce a gene of interest into the destination plasmid, recombination sites must first be added...

  9. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ... some special characteristics of scientists in the work place as described by Sebastiano Massaro in his...some of these common pitfalls of management and still not get the most from your team. It is pretty clear...learned to get results with the carrot, and not the stick.     This is the first in a 5 part series on Introduction...

  10. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...are on the right track if they start asking you questions. Remember that people like a good story and science... this case you – on a quest to ask and answer questions and hopefully discover something new. Science ...out to science writers in your area or at your institution; the news or communications office is a good ...internship at a place like Science News. Ask your institutional news office if there might be opportunities ...

  11. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...Makarova et al. grouped the systems into three distinct types and subtypes using polythetic classification... not translatable to the other CRISPR subtypes. Still, the large and dynamic range of CRISPR systems between...viral anti-CRISPR genes. These data highlight an interesting "arms race" between phages and prokaryotes; the... and further develop the CRISPR/CAS toolkit by posting generic lab protocols, providing tips from experts...

  12. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...will bind to both and you will not be able to distinguish between your two targets.    Figure 1:...primary antibodies and the targets will be indistinguishable (top panel). If one of the antibodies is converted...be converted? Isotype conversion is a unique characteristic of recombinant antibodies. Recombinant antibodies... that this blog post has answered all of your questions about the utility of isotype conversions and chimeric...

  13. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ...how to amplify recombination-prone plasmids and testing your prep can save you the headache of troubleshooting...colony you selected contains the full plasmid. A diagnostic digest is one of the easiest and clearest ways...you can try re-streaking for single colonies and testing multiple preps. In some cases, we suggest gel extraction...plasmid is present, but the recombined plasmid is still visible. This indicates that the DNA prep contains...

  14. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...University, and the Karolinska Institute developed mNeonGreen via 21 substitutions to LanYFP and the addition...Nathan Shaner and colleagues from The Scintillon Institute, Allele Biotechnology, Florida State University...applications from traditional imaging to FRET and stochastic single-molecule super resolution imaging. mNeonGreen...

  15. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...2016 Addgene’s research team has been actively investigating ways to improve viral vector production. Whether...Whether redesigning viral packaging plasmids, investigating different viral production strategies or developing...were unable to deduce the mechanism responsible we still believe that these results will be beneficial to...method to improve AAV yields. Although we only investigated 3 serotypes it’s possible that this approach...

  16. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ... have the potential to provide improvements to existing technologies based on polymerase processivity....stable, highly soluble, and non-immunogenic yet are still just as specific to their target as regular antibodies...podcast segment Steven Carr’s lab at the Broad Institute focuses on proteomics: developing and applying...analysed. In this work, the authors specifically investigated protein binding at the TERT gene, but this method...

  17. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... replicate the host's chromosomal DNA, but they still rely on the host machinery to make additional copies...Patrick says researchers can ask themselves a few questions to get started: Will the plasmid be used exclusively...that plasmids with low to medium copy numbers can still express massive amounts of protein given the proper...cells have not started to die off. Have any questions? Need something clarified? If so please, post ...

  18. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...tips for scientists working at home, and you will still find this useful. Now that we’ve been doing this...just need to know you well enough to ask good questions and hold you accountable for your goals.  I’ve...interviews are 1:1 discussions with someone who has interesting experiences to share. The conversation builds...edit everything you’ve tried to draft, but you’ll still be practicing and getting familiar with the process...

  19. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ... mountain – with many routes up and only one destination – a career in the sciences is more like an intergalactic...developmental programs unfold, absorbing the stochasticity of molecular interactions and embedding individual... weird and rare organisms so I found it hard to stick to vertebrates alone.  And a longstanding inclination...with things like word choice, punctuation, and stylistic consistency. If you’ve ever doubted the importance...

  20. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...I didn’t truly address the question of what I wanted to do when I grew up until I was all but finished...writing lab reports and empirical essays had also stirred some of my more creative faculties into motion,...skill acquisition. Classes covered filmmaking, podcasting, philosophy of science, creative writing, designing...communication graduates are often well-equipped. Many institutions are sincerely interested in connecting with ...
Showing: 541 - 560 of 1043 results