We narrowed to 583 results for: SUB;
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TypeBlog Post...the mutant MECP2 RNA in each of three neuronal subtypes of the hippocampus. This technology has formed...
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Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post...genome modification, but they did so without a substantial immune response or toxicity. More AAV-based CRISPR... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...protein tools that are activated, respectively, by substrates, light, or engineered ligands (Figure 2). Biosensors... -
Hot Plasmids Spring 2024
TypeBlog Post...-to-noise ratio and better ability to record subthreshold voltage fluctuations, while having high fidelity... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post...Enterobacter sp., Erwinia amylovora, E. carotovora subsp. Carotovora, E. herbicola, E. rubrifaciens, E. stewartii... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog Post...doxycycline-free media and expand the cells. Use a subset of the cells to verify the mARG positivity, which... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...surprising. Consortia of higher education institutions submitted letters to the Department of Labor (for example... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...decision determines whether the client invests substantial monetary and human resources towards the development... -
Tips for Using FRET in Your Experiments
TypeBlog Post... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post...polymerases which are each responsible for a specific subset of RNAs. To gain this specificity, the eukaryotic... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post... of Ccd42 (Bendezú et al., 2015) and the alpha subunit of heterotrimeric G-proteins (Janetopoulos et al... -
Hot Plasmids: Summer 2024
TypeBlog Post...nervous system is composed of myriad cell types and subtypes, and a familiar challenge for anyone who has done... -
CRISPR 101: Homology Directed Repair
TypeBlog Post... ssDNA 3’ overhang. This will serve as both a substrate for proteins required for strand invasion and ... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific... -
Evolution of Lab Techniques
TypeBlog Post...screen multiple colonies before selecting a smaller subset to sequence. Sequencing gels and next-generation... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...course of infection, when HIV-1 is limited to a subset of T-cells, but it is unclear if enough T-cells... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post...had been more cognizant of this during my PhD. Submitting a publication doesn’t have to be like throwing... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post...efficiently integrate into the DNA through an HDR subpathway. It's traditionally been difficult to produce... -
Hot Plasmids: Spring 2025
TypeBlog Post... to target and manipulate distinct interneuron subtypes. Neuron, 113(10), 1525–1547.e15. doi: https://... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post...designs and administration doses. Don't forget to submit your validation data after your experiment! Thanks...