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Showing: 541 - 560 of 576 results

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New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

Type

Blog Post

...doxycycline-free media and expand the cells. Use a subset of the cells to verify the mARG positivity, which...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

...patients from whom the blood is obtained and the subsequent reprogramming success [1]. Fibroblasts need to...
CRISPR-based Genome Editing Systems For Editing in E. coli

Type

Blog Post

...resulting in cutting of the plasmid. At this point, subsequent introductions of pTargetF containing another ...
Changing Labor Laws Bring Increased Postdoc Wages

Type

Blog Post

...surprising. Consortia of higher education institutions submitted letters to the Department of Labor (for example...
Healthcare Consulting: A Door to the Business of Life Sciences

Type

Blog Post

...decision determines whether the client invests substantial monetary and human resources towards the development...
Tips for Using FRET in Your Experiments

Type

Blog Post

... Constructs to target mTurquoise2 to various subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian...
Plasmids 101: The Promoter Region – Let's Go!

Type

Blog Post

...polymerases which are each responsible for a specific subset of RNAs. To gain this specificity, the eukaryotic...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

... of Ccd42 (Bendezú et al., 2015) and the alpha subunit of heterotrimeric G-proteins (Janetopoulos et al...
Hot Plasmids: Summer 2024

Type

Blog Post

...nervous system is composed of myriad cell types and subtypes, and a familiar challenge for anyone who has done...
CRISPR 101: Homology Directed Repair

Type

Blog Post

... ssDNA 3’ overhang. This will serve as both a substrate for proteins required for strand invasion and ...
Multiple Plasmids at a Low Price: Kits from Addgene

Type

Blog Post

...used to purify proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific...
Evolution of Lab Techniques

Type

Blog Post

...screen multiple colonies before selecting a smaller subset to sequence. Sequencing gels and next-generation...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

...course of infection, when HIV-1 is limited to a subset of T-cells, but it is unclear if enough T-cells...
Thoughts on the Future of Research 2015 Boston Symposium

Type

Blog Post

...had been more cognizant of this during my PhD. Submitting a publication doesn’t have to be like throwing...
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Type

Blog Post

...efficiently integrate into the DNA through an HDR subpathway. It's traditionally been difficult to produce...
Hot Plasmids: Spring 2025

Type

Blog Post

... to target and manipulate distinct interneuron subtypes. Neuron, 113(10), 1525–1547.e15. doi: https://...
Antibodies 101: Multiplex Immunofluorescence

Type

Blog Post

... J., & Trimmer, J. S. (2019). A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...
PiggyBac-ing Through the Genome Editing Field

Type

Blog Post

...marker such as thymidine kinase, a researcher may subsequently kill off any cells that still contain the donor...
Antibodies 101: Validation

Type

Blog Post

...antibody that you want to use to look at the subcellular localization of a protein of interest. Conveniently...
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

Type

Blog Post

...non-homologous sequence or a series of single-nucleotide substitutions along a 1.5 kb tract, all without selectable...

Showing: 541 - 560 of 576 results

Results per page:

New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

CRISPR-based Genome Editing Systems For Editing in E. coli

Changing Labor Laws Bring Increased Postdoc Wages

Healthcare Consulting: A Door to the Business of Life Sciences

Tips for Using FRET in Your Experiments

Plasmids 101: The Promoter Region – Let's Go!

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Hot Plasmids: Summer 2024

CRISPR 101: Homology Directed Repair

Multiple Plasmids at a Low Price: Kits from Addgene

Evolution of Lab Techniques

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Thoughts on the Future of Research 2015 Boston Symposium

Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Hot Plasmids: Spring 2025

Antibodies 101: Multiplex Immunofluorescence

PiggyBac-ing Through the Genome Editing Field

Antibodies 101: Validation

An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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