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...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...

...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...

... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...

... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

...antibiotic resistant bacteria have previously flourished.   Figure 2: Two-phage strategies to resensitize...

...with a molecule of interest (the ‘bait’) can be ‘fished out’ for further study. In Y2H screens, a group...

...about Cpf1 and check out our CRISPR guide for a refresher on CRISPR/Cas9.  Figure 1: Cpf1 is a single...

...structure, first reported in 1996, is an eleven β-sheet-containing “barrel” shape, with the chromophore ...

...medium such as Opti-MEM or Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear...

... clinical grade hiPSCs is sold at $11,000 at ThermoFisher Scientific and can be used for only 6-8 reprogramming...

.... PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent Biosensors...

...include every piece of information that was ever published. Obviously this isn’t possible. So, define your...

...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...

...might help to get you started. Once you have established a well working protocol in your lab, it can become...

...our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR eBook Resources on Addgene....

...exist to solve the problem. What will you have accomplished in 5-10 years? You need to be able to describe...

... Alyssa Shepard...

...sequences are translated into amino acids. And I was astonished that the molecular language shared by every living...

...References and Resources Glossary Here’s a quick refresher if you ever lose track of which acronym is which...

...our educational resources. From 2013–2023, we published 110 videos, which received over two million views...