We narrowed to 611 results for: She

... selected and ranked based on the previously established sgRNA rules and  potential on- and off-target...

...specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors...

...vector. For example, although researchers know that Usher Syndrome (a disease that results in combined deafness...

...their N-terminal His14-bdNEDD8 tag. After several washes, this tag can be cleaved by the bdNEDP1 protease...

... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...

...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

...Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response...

...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...

...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...

...antibiotic resistant bacteria have previously flourished.   Figure 2: Two-phage strategies to resensitize...

...with a molecule of interest (the ‘bait’) can be ‘fished out’ for further study. In Y2H screens, a group...

...about Cpf1 and check out our CRISPR guide for a refresher on CRISPR/Cas9.  Figure 1: Cpf1 is a single...

...structure, first reported in 1996, is an eleven β-sheet-containing “barrel” shape, with the chromophore ...

.... PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent Biosensors...

...include every piece of information that was ever published. Obviously this isn’t possible. So, define your...

...might help to get you started. Once you have established a well working protocol in your lab, it can become...

...our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR eBook Resources on Addgene....

...exist to solve the problem. What will you have accomplished in 5-10 years? You need to be able to describe...

...References and Resources Glossary Here’s a quick refresher if you ever lose track of which acronym is which...