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Protocol - How to Ligate Plasmid DNA

Type

Protocol

...compatibly digested vector backbone. This is accomplished by covalently connecting the sugar backbone ...
Plasmids 101: Shuttle Vectors

Type

Blog Post

...sequences are translated into amino acids. And I was astonished that the molecular language shared by every living...
CRISPR 101: Any Base Transversion Editors

Type

Blog Post

...References and Resources Glossary Here’s a quick refresher if you ever lose track of which acronym is which...
Twenty Years of Sharing: Expanding Accessibility

Type

Blog Post

...our educational resources. From 2013–2023, we published 110 videos, which received over two million views...
Twenty Years of Sharing: Addgene's Viral Vector Service

Type

Blog Post

...work — in their system. The data is curated and published with a DOI, allowing others to reference and cite...
Designing Your Chalk Talk for the Academic Job Interview

Type

Blog Post

...exist to solve the problem. What will you have accomplished in 5-10 years? You need to be able to describe...
Fluorescent Proteins 101: Aptamer Fluorophores

Type

Blog Post

.... PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent Biosensors...
CRISPR Activation: A Practical Guide

Type

Blog Post

...Cassel SH, Vazquez F, Weir BA, Hahn WC, Marsh JA, Tsherniak A, Kadoch C (2018) Interrogation of Mammalian ...
Getting the Most from Your Lentiviral Transduction

Type

Blog Post

...possible transduction. Be consistent Once your lab establishes the best conditions for specific applications...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

... clinical grade hiPSCs is sold at $11,000 at ThermoFisher Scientific and can be used for only 6-8 reprogramming...
How to Write a Scientific Review Article

Type

Blog Post

...include every piece of information that was ever published. Obviously this isn’t possible. So, define your...
Save Time with Transient Plant Leaf Transformations

Type

Blog Post

...might help to get you started. Once you have established a well working protocol in your lab, it can become...
New Tools Enable CRISPRa for Neuroscience Applications

Type

Blog Post

...our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR eBook Resources on Addgene....
Plasmids 101: Broad Host Range Plasmids

Type

Blog Post

..., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas campestris...
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

Type

Blog Post

...express Anabaena GVs in mammalian cells without the shell stiffening protein GvpC, which allowed the GVs to...
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Type

Blog Post

...have been used successfully in experiments and published in peer reviewed journals. If you need to generate...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...
Plasmids 101: E. coli Strains for Protein Expression

Type

Blog Post

...better at 30°C or room-temperature, which is accomplished by growing your culture to the desired density...
Hot Plasmids: Summer 2024

Type

Blog Post

..., particle orientation distribution and Fourier-shell correlation curve (D), and the reconstructed 3D ...
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

Type

Blog Post

...dermis and identify that Langerhans cells are replenished through the proliferation of specific, and not...

Showing: 541 - 560 of 596 results

Results per page:

Protocol - How to Ligate Plasmid DNA

Plasmids 101: Shuttle Vectors

CRISPR 101: Any Base Transversion Editors

Twenty Years of Sharing: Expanding Accessibility

Twenty Years of Sharing: Addgene's Viral Vector Service

Designing Your Chalk Talk for the Academic Job Interview

Fluorescent Proteins 101: Aptamer Fluorophores

CRISPR Activation: A Practical Guide

Getting the Most from Your Lentiviral Transduction

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

How to Write a Scientific Review Article

Save Time with Transient Plant Leaf Transformations

New Tools Enable CRISPRa for Neuroscience Applications

Plasmids 101: Broad Host Range Plasmids

New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Plasmids 101: E. coli Strains for Protein Expression

Hot Plasmids: Summer 2024

Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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