We narrowed to 605 results for: She

1. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   ... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...

2. Isolating Genomic Regions of Interest with the CRISPR System

   Type

   Blog Post

   ...our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org Read our CRISPR guide...

3. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

   Type

   Blog Post

   ...genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa/i,...

4. A Guide to Starting Your Own Journal Club

   Type

   Blog Post

   ...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...

5. An Introduction to Adenovirus

   Type

   Blog Post

   ...Kafri, T., Morgan, D., Krahl, T., Sarvetnick, N., Sherman, L., & Verma, I. (1998). Cellular immune response...

6. Course-Based Undergraduate Research Experiences (CUREs)

   Type

   Blog Post

   ...Molecular Biology Annual Meeting. Some of my students published their work identifying new genes required for ...

7. Tips for Screening with Yeast Two Hybrid Systems

   Type

   Blog Post

   ...with a molecule of interest (the ‘bait’) can be ‘fished out’ for further study. In Y2H screens, a group...

8. Cpf1: A New Tool for CRISPR Genome Editing

   Type

   Blog Post

   ...about Cpf1 and check out our CRISPR guide for a refresher on CRISPR/Cas9.  Figure 1: Cpf1 is a single...

9. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   ...structure, first reported in 1996, is an eleven β-sheet-containing “barrel” shape, with the chromophore ...

10. Lentivirus Production

    Type

    Protocol

    ...medium such as Opti-MEM or Opti-Pro SFM, Thermo Fisher, 12309019 25 mM chloroquine Polyethylenimine, linear...

11. Fluorescent Proteins 101: Aptamer Fluorophores

    Type

    Blog Post

    .... PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent Biosensors...

12. How to Write a Scientific Review Article

    Type

    Blog Post

    ...include every piece of information that was ever published. Obviously this isn’t possible. So, define your...

13. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type

    Blog Post

    ... clinical grade hiPSCs is sold at $11,000 at ThermoFisher Scientific and can be used for only 6-8 reprogramming...

14. CRISPR Activation: A Practical Guide

    Type

    Blog Post

    ...Cassel SH, Vazquez F, Weir BA, Hahn WC, Marsh JA, Tsherniak A, Kadoch C (2018) Interrogation of Mammalian ...

15. Getting the Most from Your Lentiviral Transduction

    Type

    Blog Post

    ...possible transduction. Be consistent Once your lab establishes the best conditions for specific applications...

16. Save Time with Transient Plant Leaf Transformations

    Type

    Blog Post

    ...might help to get you started. Once you have established a well working protocol in your lab, it can become...

17. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    ...our CRISPR blog posts Download our CRISPR cheat sheet Download our CRISPR eBook Resources on Addgene....

18. Designing Your Chalk Talk for the Academic Job Interview

    Type

    Blog Post

    ...exist to solve the problem. What will you have accomplished in 5-10 years? You need to be able to describe...

19. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    ...Originally published Nov 12, 2015 and last updated Aug 20, 2020. Cas9 can be used to modify any desired...

20. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type

    Blog Post

    ...have been used successfully in experiments and published in peer reviewed journals. If you need to generate...