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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 937 results for: TIM;

Showing: 541 - 560 of 937 results

  1. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...certain number of days and tracking average transit time for each courier we contracted with so we can always...navigated customs and arrived at their destination in a timely manner and at the correct temperature. To date,...written resources are valuable, there does come a time when a picture is worth a thousand words, especially...

  2. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...Addgene repository can save researchers significant time and removes a variable from the lengthy process ... patterns so different? It could be a matter of timing, as jGCaMP7 was released relatively soon after ...informed choice on which AAV to request and/or save time in selecting experimental conditions. Sharing our...

  3. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...authors) are currently working on improving and optimizing ARGs, with Marjorie focused on bacterial ARGs...imaging (Fig. 2d). Creating a cell line can be a time-intensive endeavor. That is why we also included...Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria...

  4. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...therapeutic applications where longer retention times often increase therapeutic efficacy (Ahmed et al...re-solubilize and re-fold scFvs from inclusion bodies, it’s time-consuming and laborious.  To avoid this, one or ...https://doi.org/10.3390/antib2020193  Khantasup K, Chantima W, Sangma C, Poomputsa K, Dharakul T (2015) Design...

  5. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ... videos for free. We’re a small operation, so sometimes it can be a big challenge. But ask any filmmaker...was out the window. So, much like SynBio, it was time to start our Design-Build-Test-Learn cycles. If ...everyone through the recording process was going to be time consuming and difficult. Finding volunteers who ...

  6. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...to serve as a primer for DNA repair synthesis, ultimately leading to the formation of double Holliday junctions...Challenges: Standardization and HDR CrispyCrunch Optimizing Donor DNA for Enhanced CRISPR Genome Editing ...Methods in Enzymology Vol. 546; (2015). PubMed. Optimization of Genome Engineering Approaches with the CRISPR...

  7. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...imaging of genomic loci in living human cells by an optimized CRISPR/Cas system." Cell 155.7 (2013): 1479-1491... al. "Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging." Proceedings of...29472486. 17. Adjobo-Hermans, Merel JW, et al. "Real-time visualization of heterotrimeric G protein Gq activation...

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    .... Digestion with enzyme “B” detects primer multimerization (purple) which is evident by an increase in...manufacturers’ PCR protocol. Be sure to adjust the extension time to match the size of your template. Amplification...CRISPR-induced genome modifications. Occasionally, multimerization of the PCR primers can cause duplication of...

  9. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...tweets from the Symposium under #FORBOS15) - my first time live tweeting: a fun and surprisingly exhausting...why they are paid relatively low wages (Stephan estimates that postdocs are paid $16.15/h before fringe ... is certainly true that not all PIs can take the time to do this, you should always reach out to your ...

  10. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ... IF, where one target is labeled and imaged at a time, inactivating the fluorescent secondary antibodies...antibody may be binding non-specifically – try optimizing your blocking and washing conditions or consider...-reactivity. Keep in mind that you may need to optimize the conditions and concentrations of all the different...

  11. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...that sometimes it is necessary or desirable to control or look at multiple genes at the same time.  The...transcription of a target gene, it therefore determines the timing of gene expression and largely defines the amount...

  12. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...same pace and enter the resolving gel at the same time where they are then separated by size. Gradient ...concentrations, blocking buffers, and incubation times. If the vendor includes publications that cited ...   Analyzing the western blot Once visible, it’s time to analyze your protein of interest. Analysis typically...

  13. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...evidence of this, you needn’t look any further than testimonials from the students who participated in the program...mentee’s personality and professional needs at any time. In its Pilot Program 2014-15, IMP provided mentorship... monthly progress video conferences and had real-time correspondence via e-mail throughout the entire ...

  14. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...silencing efficacy, thereby requiring a substantial time investment. Once the target mRNA is cleaved, the...and robust gene silencing (following initial optimization of the amiRNA). Although downstream work is ... manually extracted. This process is tedious and time consuming, as RNAi mutants with a reduced growth...

  15. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...SNAP-OMP25 with Atto590-BG (green), imaged with stimulated emission depletion (STED) nanoscopy. Scale bar...updating your labels easy! Instead of taking the time to clone a new construct for every new-and-improved...switching between different colored ligands at different time points.   Figure 3: Single-molecule tracking...

  16. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...of protein function. In addition, the level and timing of expression can also be affected by the tag.  ...immune complexes will no longer recognize them. Sometimes, users will combine methods and fix with formaldehyde...health and limit debris, keep samples on ice at all times and use gentle pipetting instead of vortexing. Include...

  17. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...a large majority of neuronal cells are labeled overtime due to the continued replication of the virus....have been widely used by neuroscientists for some time due to their advantageous characteristics for brain... titration on the Addgene website).  Tissue Optimal Serotype CNS AAV1, AAV2, AAV4, AAV5, AAV8, ...

  18. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances ....G This plasmid has been requested nearly 6,000 times (over 800 orders in 2017) and expresses the VSV-...

  20. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ... have amazing stories to share. I spend a lot of time telling scientists about Addgene’s repository, and... and request plasmids, but I also spend a lot of time listening. I’ve had hundreds of conversations with...
Showing: 541 - 560 of 937 results