We narrowed to 888 results for: TRI

...approaching it. These include the traditional restriction enzyme/ligase-mediated method, the more recently.../19 vectors and demonstrating the concept of restriction enzyme-mediated disruption of ccdB, leading to...

...This post was contributed by guest blogger Alan Wong. The complexity of biological systems can hinder...iterative one-pot reactions with straightforward restriction digestion and ligation steps to build barcoded...

...photostable YFP to date. As such, mGold is expected to contribute to experiments that require high photostability...GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth...

...This post was contributed by guest blogger Daniel M. Sapozhnikov, a graduate student in the laboratory...but if this is not possible due to gRNA design restrictions, anything within 5 base pairs should work well...

...although both are of course preferred! When trying to strike that balance, you may wonder: how close is close...significant amount of flexibility compared to a strict single-compatibility sequence like NGG. Nonetheless...

...green, blue, red, and far-red. There is also a ratiometric GECO sensor (GEX), where one wavelength changes... working with a stably integrated biosensor. Ratiometric measurements with certain single FPs (such as...

...References and resources References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application...(08)60255-1  Manning, C. F., Bundros, A. M., & Trimmer, J. S. (2012). Benefits and Pitfalls of Secondary...

.... These plasmids have reached labs in over 75 countries. Figure 1: SARS-CoV-2 proteins and other... after reverse transcription (RT-LAMP) and a colorimetric readout that can be completed in just 45 minutes...

...laboratory of Karmella Haynes at Emory recently contributed a set of plasmids to the repository that will... function in receiver cells. There are over 40 trillion cells that make up the human body and G-baToN ...

...This post was contributed by guest blogger Clare O'Connor an Associate Professor at Boston College. National...plasmids are also used to teach students about restriction mapping and western blotting. Students who participate...

... to prep large quantities of DNA because some countries just can’t receive bacteria through the mail due..., if you have any great lab automation tips and tricks, be sure to share them in the comments section....

...efficiency and any unpaired fluorescent proteins can contribute additional noise to the measurement. If the distance... FRET experiments are expected to continue to contribute to our understanding of diseases and even assist...

...plays an important role in RNA processing and contributes to variability in RNA half-life, and ultimately... Rho-independent termination is also known as intrinsic termination, and it relies on the formation of...

...in-situ hybridization, methylation-sensitive restriction enzyme digestion, DNA adenine methyltransferase...Cardiovascular and diabetes mortality determined by nutrition during parents' and grandparents' slow growth ...

...This post was contributed by Brittany L. Uhlorn, a PhD Candidate at the University of Arizona. Perhaps... next poster session! There many other tips and tricks for presenting at scientific conferences, so let...

...standard curves, and normal controls must be run in triplicate.  The nice thing about qPCR is that since it ... of reactions, it does not need to be run in triplicates. ddPCR is an extremely accurate application, ...

...license. If you’re currently using mVenus or mCitrine, or if existing yellow fluorescent proteins just...anterograde labeling across single synapses, a key attribute for neuronal tracing. Labeling was nontoxic and...

...genes for drug discovery or to understand the intricacies of molecular pathways. These screens have gotten...solutions have been found to get around this restriction. The most obvious workaround is to use a multi-plasmid...

...This post was contributed by Greg Dingle, a software engineer with the Chan Zuckerberg Initiative. We...McMaster University in Canada. He's happy to be contributing again to science.   Acknowledgements Chan Zuckerberg...

...Updated Apr 6, 2021. This post was contributed by Laura Desrochers, a postdoctoral scientist at AstraZeneca... academic lab is closely involved with clinical trials or has ties to industry, the drug discovery process...