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Addgene

We narrowed to 746 results for: UTY

Showing: 541 - 560 of 746 results
  1. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...EDTA Store Solution I at 4°C Solution II - Denaturing Solution 0.2 N NaOH 1.0% SDS Store Solution II at room...plasmid Resuspension buffer Denaturing solution Renaturing solution 2 mg/mL RNase A TE or water-saturated...that is compatible with the next solution. Add a denaturing solution to the resuspended bacteria. Note...contents, including plasmid DNA, into solution. Add a renaturing solution to the denatured bacteria. Note: ...Lysis Plasmid Miniprep Prepare the following solutions: Solution I - Resuspension Buffer 25 mM Tris-HCl (pH... room temperature Solution III - Renaturing Solution (Potassium Acetate) 120 mL 5M Potassium acetate 23...Resuspend the pellet in 100 μL of cold Solution I. Vortex the solution for 2 min or until all bacteria are...
  2. Colony Formation Titering Assay

    Type
    Protocol
    ... Mix the dilutions well Note: the 1:10 dilution can usually be omitted because this dilution typically... serial dilutions of the lentivirus into DMEM complete containing 10 μg/mL polybrene: Dilution Volume ...quantified. Add 150 μL of a viral dilution to each well (each well gets one dilution) Also include an untransduced... colonies for at least 2 of the dilutions. Pro-Tip The higher dilution wells tend to provide more accurate...present in the lower dilutions, and single colonies are visible in the higher dilutions. Last reviewed on...staining resistant colonies with crystal violet solution and, therefore, the cells cannot be used for later...DMEM complete containing 10 μg/mL polybrene by diluting 20 μL of 10 mg/mL polybrene into 20 mL of media...
  3. Viral Vectors 101: Transductions

    Type
    Blog Post
    ... Molecular basis of transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into...
Showing: 541 - 560 of 746 results