We narrowed to 746 results for: UTY
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TypeBlog Post...History of CRISPR Cas - A tale of survival and evolution ...
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Handling Plasmids from Addgene - Purifying Plasmid DNA
TypeProtocol...EDTA Store Solution I at 4°C Solution II - Denaturing Solution 0.2 N NaOH 1.0% SDS Store Solution II at room...plasmid Resuspension buffer Denaturing solution Renaturing solution 2 mg/mL RNase A TE or water-saturated...that is compatible with the next solution. Add a denaturing solution to the resuspended bacteria. Note...contents, including plasmid DNA, into solution. Add a renaturing solution to the denatured bacteria. Note: ...Lysis Plasmid Miniprep Prepare the following solutions: Solution I - Resuspension Buffer 25 mM Tris-HCl (pH... room temperature Solution III - Renaturing Solution (Potassium Acetate) 120 mL 5M Potassium acetate 23...Resuspend the pellet in 100 μL of cold Solution I. Vortex the solution for 2 min or until all bacteria are... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog Post... you’re interested in (i.e. biotechnology, pharmaceuticals, etc.) Do their jobs sound interesting? Is ... -
An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog Post... responsible for establishing a new report and routine across the marketing and content team. Initially... -
Antibodies 101: Fc Effector Functions
TypeBlog Post... induce? Fc receptors are intriguing from a therapeutic standpoint. Engineered monoclonal antibodies ... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog Post...proficient and deficient for the target. CRISPR has revolutionized the generation of knockout cell lines which... -
Addgene at the ASM Microbe 2016 Conference
TypeBlog Post... to find out more about our free deposit and distribution service! Did you see a must-have plasmid during... -
Understanding GPCR Signaling Using TRUPATH
TypeBlog Post...well known and, as a result, a target of many pharmaceutical drugs, there is still only a partial understanding... -
Bricking Science: Portraying Scientific Reality Through LEGO
TypeBlog Post...people the very human struggles in the laboratory routine. We hope that sharing and laughing at these struggles... -
FastCloning: A Newer, Simpler Cloning Technique
TypeBlog Post...transformation. While restriction cloning is still routinely performed by many labs, a variety of other cloning... -
Management for Scientists: Managing vs. Leading
TypeBlog Post...take the initiative by bringing and effecting solutions to both known and unidentified issues. There are... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...expression at an opportune time or go all out with constitutive high level expression? Finally, you can add ... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post... your fingers in during a run or risk minor electrocution. These days you can buy small gel boxes, but... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...stable in powder form, but quickly breakdown in solution. Storing aliquots at -20oC and avoiding repeated... -
Colony Formation Titering Assay
TypeProtocol... Mix the dilutions well Note: the 1:10 dilution can usually be omitted because this dilution typically... serial dilutions of the lentivirus into DMEM complete containing 10 μg/mL polybrene: Dilution Volume ...quantified. Add 150 μL of a viral dilution to each well (each well gets one dilution) Also include an untransduced... colonies for at least 2 of the dilutions. Pro-Tip The higher dilution wells tend to provide more accurate...present in the lower dilutions, and single colonies are visible in the higher dilutions. Last reviewed on...staining resistant colonies with crystal violet solution and, therefore, the cells cannot be used for later...DMEM complete containing 10 μg/mL polybrene by diluting 20 μL of 10 mg/mL polybrene into 20 mL of media... -
Viral Vectors 101: Transductions
TypeBlog Post... Molecular basis of transduction Transductions utilize the ‘normal’ mechanism of viral infiltration into... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog Post...cabbage and kale to name a few. Despite Arabidopsis’ utility to science, and its well-known edible relatives... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...instability can cause partial loss of an ITR during routine plasmid propagation, with complete ITR loss abolishing... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...scripts with laboratories and viral vector cores routinely producing AAV. We believe that this simplified...