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We narrowed to 841 results for: ang

Showing: 541 - 560 of 841 results

  1. The time and cost required to make a plasmid

    Type
    Blog Post
    ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...harder it is to throw in last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means...

  2. To Each HIS Own

    Type
    Blog Post
    ...and several contaminating proteins (blue, green, orange) flows through the Ni2+-containing resin. The protein...polyhistidine containing contaminating protein (orange). A low imidazole wash buffer disrupts the weak...

  3. All in a Twist: dsRNA

    Type
    Blog Post
    ... hybrid molecules both exist and have a diverse range of functions within mammalian cells. Here, we will... much more! Resources and References References Wang, I. X., Grunseich, C., Fox, J., et al., Human proteins...

  6. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...abilities is that its fluorescence emission spectra changes when it goes from a more neutral pH compartment...emission spectra. Component Excitation Emission pH Range DsRed.T3 488, 543*, 568 nm 587 nm ~4.9 - ...

  7. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...would be the effects, positive and negative, of changing the workforce structure? Helping to answer some...Roach. "Science PhD career preferences: levels, changes, and advisor encouragement." PloS one 7.5 (2012...

  8. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...that cause disease. While these plasmids can be dangerous, they also provide researchers with new means ..., Moody, T., Rice, P. A., Yu, M. K., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan...

  9. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...means that each component can be designed with an overhang specific to the type of module it is (Pol 2 protomer...for the Fragmid cuts, clone in the appropriate overhang for your module type, and voilà! You’ll have your...

  10. Hot Plasmids February 2024

    Type
    Blog Post
    ...Adapted from Zhang et al. 2023 with permission.   Find Cas-PAGE plasmids here!   Zhang, Z., et al....

  11. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...associated with low morbidity and mild symptoms. This changed in January with the report of an outbreak of the...Learn How Minigenomes Are Being Used to Study Dangerous Viruses Resources on Addgene.org Check out All...

  12. Rabies and Neuronal Tracing

    Type
    Blog Post
    .... It wasn’t until later time points that longer-range connections were seen. This study addressed the ...Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, Tsiang H, Ceccaldi PE. Spread and pathogenic characteristics...

  14. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... DNA in a number of ways, from small edits that change single base pairs, to chromosomal inversions and... S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, ...

  15. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    .... V., Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J...–375. https://doi.org/10.1038/s41586-023-06570-y Wang, J., Gessler, D. J., Zhan, W., Gallagher, T. L.,...

  16. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...titration by qPCR.   Pro tip! An optional media exchange can be performed 12–18 hours post-transfection...the density gradient material by dialysis/buffer exchange and further concentrate the virions if needed....

  17. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...infects host cells through binding the human angiotensin converting enzyme 2 (ACE2) cell-surface receptor....01.30.428974   The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids...

  18. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...of an exogenous small molecule). Degron tags can range in size from several kDas up to ~35 kDa and are ...10.1038/s41467-022-33246-4 Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper RK, Tsien RY, Lin MZ. Tunable...

  19. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...same type will produce the same unique SapI 5’ overhang, different combinations of connector and tag donor...interactions. Additionally, thanks to the modular arrangement of vectors included in tagging systems like SapTrap...

  20. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...stranded breaks, in which nucleotide sequences are exchanged between two similar or identical molecules of ...and animal models, their efficiency is very low, ranging from undetectable to 0.1%. In contrast, new genome...
Showing: 541 - 560 of 841 results