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We narrowed to 803 results for: mak

Showing: 541 - 560 of 803 results

  1. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...Multi-color labeling of neurons is for more than making pretty pictures (see figure 4). It also enables...a specific cell type in the brain, you needed to make a transgenic mouse. Combining AAv.PHP.eB’s or AAV.PHP.S...

  2. DNA Purification Without a Kit

    Type
    Blog Post
    ...powder slurry that has a milk-like appearance. To make DNA stick to the glass particles in glass milk, ...but the Zhao and Deng labs found they could just make their own buffers when the kits buffers ran out....

  3. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...our samples 1:6 million to 1:25 million.  After making the dilutions, they are transferred to a new plate...generator, you should avoid pipetting too quickly when making your virus dilutions. This will reduce aerosols...

  5. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...tumors. Lentiviral vectors are also commonly used to make stable transgene-expressing or knockout cell lines...clinical stages. Researchers are also working to make lentiviral vectors safer. Since lentiviral vectors...

  6. Plasmids 101: Protein tags

    Type
    Blog Post
    ...resources are being spent to make something the cell doesn’t need to make. Eukaryotes and some bacteria...

  8. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...the list of antibiotic options doesn’t end there. Make sure you read the instructions for whatever antibiotic...kingdoms of life. A bit of careful design will help make your experiment with shuttle vectors a success! ...

  9. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...buffer components can interfere with conjugates. Make sure to do your research before conjugating at the...The assay only uses one antigen binding antibody, making it less specific than other methods. In addition...

  11. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo...S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F (2019) Engineering of CRISPR-Cas12b...

  13. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...edits but the double strand breaks it introduces makes it difficult to perform two or more integrations...targeting another site of interest can be used to make other edits. Once you have all the edits you want...

  15. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...standard curve of known concentration. We regularly make and validate fresh plasmid standards. We validate...determine accuracy and reliability. When doing this, we make a judgement call on whether the two titer values...

  16. Hot Plasmids - November 2023

    Type
    Blog Post
    ...effective and with no additional off-target edits, making them ideal for size-limited AAV delivery. PE6c ...and MMLV gag, to specific cell types. It can also make CAR-T cell therapy more accessible by simplifying...

  17. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...for whom English is a second or third language (making publishing their research all the more challenging...Washington in St. Louis, offer guidance on how to make a low-cost whiteboard video about your research....

  19. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...microscopy techniques. FRET measurement sensitivity makes it suitable for studying interactions within living...approaches and appropriate controls are required to make this method useful for dynamic experiments in which...

  20. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...replication are necessary to replicate plasmids and make sure the cells maintains it. Here, we'll cover the...on type of RNA transcript, you will also need to make sure your plasmid has a promoter suited to working...
Showing: 541 - 560 of 803 results