...amplification using cloned thermostable ligase." Proceedings of the National Academy of Sciences 88.1 (1991...

...with alumni before and during your application process so you can learn more! Figure 2: The many...

...Bonferroni Correction and the Benjamini-Hochberg procedure are designed to reduce false positive rates and...

...identified as your target protein, allowing you to proceed with confidence.  Share your results Ultimately...

...to study gene regulation during developmental processes, CRISPR/Cas9 allows us to delete sequences and...

...alternatives to Cas9. Cas12 is unique because it can process multiple gRNAs under a single promoter, allowing...

...genes, Green Listed can help make the gRNA design process quick and easy. Green Listed - a CRISPR Screen ...

...UV-inducible green-to-red fluorescence conversion." Proceedings of the National Academy of Sciences of the United...

... Encouraged by the high efficiency and easy procurement of cr:tracrRNA reagents, we scaled up these tagging...

...purposes, antibodies can be used to affect cellular processes in vivo, and these are referred to as functional...

...with deconvolution, a mathematical software post-processing step that reassigns out of focus light to its...

...Ca2+ measurements using improved cameleons." Proceedings of the National Academy of Sciences 96.5 (1999...

...surgeries and viral expression, you are ready to proceed to the material science part of optogenetics. In...

...timing, and reversibility of the genome editing process. Read on to learn about the various light-controlled...

...repair. Black arrows indicate natural cellular processes. BER = base excision repair. Note: hypoxanthine...

... light to alter cell physiology and molecular processes via genetically encoded proteins. PhoCl is a new...

.... They can be stored at your bench during the procedure and at 4 °C when you’re not running a western....

...high background or low signal during the imaging process, try adjusting your exposure time first, since ...

...organs. AAV.CAP-B22 was developed through the same process as AAV.CAP-B10 and shows high transduction levels...

...randomized reorganization process, creating a unique binding site. This process, known as V(D)J recombination...delaying a process through sequential rounds of validation. Validation of an antibody is a process separate...binding partners, location in the cell, and sample processing can hide or reveal epitopes on an antigen. Monoclonal...can interfere with the conjugate or conjugation process. Sodium azide, an antimicrobial agent, can block...proteins to detect simultaneously. Conjugation The process of attaching signaling molecules to an antibody...available. Tissue samples may need additional processing steps, such as sonication. Denature proteins,...proteins from a complex mix. Overview of application Process the tissue or cell culture samples to extract proteins...