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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 922 results for: tro

Showing: 541 - 560 of 922 results

  1. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... recombinase.   Once the SSR of choice is introduced, recombination can proceed either by first utilizing...alone rescues the phenotype being studied. Introduction of a conditional point mutation or functional...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ensure...

  2. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...replacements, small insertions, and deletions) without introducing DNA double-strand breaks (Anzalone et al., 2019... a multitude of configurations can be used to introduce specific target modifications. Since this means...University of Zurich (Switzerland), where he developed a strong passion for computational biology. His expertise...

  3. PhD Applications After COVID

    Type
    Blog Post
    ...selecting an advisor, it is always a good idea to introduce yourself to a few members of your desired department... potential PI should go something like this: Introduce yourself, brief educational background, and that... meetings can still be a great opportunity to introduce yourself and learn more about your schools of ...

  4. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...background without an introduction. If someone approaches your poster, introduce yourself and ask about...have extra time to practice at the actual venue. Introduce yourself: I always find it odd when I walk up ...

  5. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...pitch,” the 30 - 60 second blurb you will use to introduce yourself and what you do. You might want to include...research. Try to avoid jargon in this initial introduction. During the conference When you meet someone...is what their project is. Or maybe you want to introduce yourself to a scientist whose work you are familiar...

  6. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...resistance gene using Cre recombinase. If GFP is under control of an endogenous promoter, you can use expression...the resistance cassette has been placed into an intron.    Figure 4: A targeting vector to create... Figure 4. Since the loxP sites are located in intronic regions, this gene will still be expressed. You'd...

  7. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...evaluated what lessons I had learned – call it “troubleshooting.” Trying out new experiments and new ideas ...erlenmeyer flask with gold glitter spray paint to make a trophy for the winner. We promoted the contest for about...online science communicators like Phil Plait @BadAstronomer, Emily Graslie from The Brain Scoop YouTube ...

  8. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...complicated cloning of homology arms. MMEJ: An Introduction There are three primary methods for repairing...NHEJ is error-prone, such a system is likely to introduce additional nucleotides flanking the insertion....the DNA ends anneal incorrectly, MMEJ may also introduce, substitute, or delete nucleotides in addition...

  9. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...comment, annotate, and edit protocols. After my introduction to protocols.io, I decided to research what ...orders, tracking students and research projects, electronic lab notebooks and protocol management. Though...More Reading: How to manage scientific data: Electronic lab notebooks compared with Google Docs and wikis...

  10. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...average of 4 sgRNAs per gene and 1,000 non-coding control sgRNAs. This library was designed to improve on-target...CRISPRko libraries are highly effective at identifying strong hits in genetic screens, they do have several limitations... Luke A., et al. "Genome-scale CRISPR-mediated control of gene repression and activation." Cell 159.3 ...

  11. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...impossible for a career scientist such as Zimmer to introduce any one of these topics without some editorializing...fellow scientists--but that doesn’t make his introduction to these topics any less effective. Who is this...non-scientists in your life, the book may be the introduction they need to grasp the challenges you face in...

  12. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ... and negative (gray) droplets. The no template control wells should have very few positive droplets. In...positive copy per microliter in the no template control wells. You’ll also notice a reduction in the number...assay, so cross contamination into a no template control (NTC) well is common. Here are some steps you can...

  13. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...IgG antibody structures. Functional comparison Strong but Large: Advantages of Antibodies One of the ...are great indirect immunodetection tools is their strong signal strength. This signal strength is due in...ladders from Penn State University Use Nanobodies to Control Protein Activity Learn more about protein tags ...

  14. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...preferred platform before you start can save a lot of trouble in the long run.  Selecting the method Once you... Requires regular content production. Internet "trolls"   More resources on the Addgene blog:  Summer...  Summer SciComm Series: Cognitive LoadIntro to our Summer SciComm Series    ...

  15. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...standpoint, a plasmid with all four genes and necessary control elements for each would be relatively large. Cloning...separate plasmids allows researchers to partially control expression levels of each gene by altering the ...genes (either viral genes or minigenome) are under control of a T7 RNA polymerase promoter sequence. Thus,...

  16. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...Laboratory Golden Gate Cloning videos made by the Nicola Patron group, one of the developers of the MoClo system...extracting and sequencing the plasmid, or both! We strongly encourage you to sequence the entire insert region...direction (which is not easy to see in colony PCR electrophoresis), is free of undesired mutations, and that ...

  17. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... for saturation mutagenesis, and they provide a strong proof of concept for further development. ...excision repair pathway. The team behind AYBEv3 introduced the first deaminase-free base editor when they... called gTBE and gCBE, with Addgene. gTBE can introduce T → C and T → G edits, though with limited selectivity...

  18. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...degraders. But CRBN has been difficult to study in vitro, requiring either expressing it with additional ...codon-optimized for C. elegans and included artificial introns to improve expression. When using these constructs... an edited strain carrying Cas9 to allow the introduction of additional sgRNAs. Image reused from Neo ...

  19. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...a large number of requests for vectors in the “controls” category, which includes things like viral vectors...vectors in a variety of serotypes, including the retrograde AAVrg and the systemic capsid PHP.eB.   ...Patrick, M., Guerin, K., & Fan, M. (2023). Quality control for Adeno-associated viral vector production. Neuromethods...

  20. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...provided a major toehold into understanding how genes control biology (a fact celebrated by the Nobel prize committee...transgenics Creating transgenic organisms by introducing foreign DNA into the genome is useful for a variety...into adults and explain how signaling pathways control this process. Additional zebrafish applications...
Showing: 541 - 560 of 922 results