We narrowed to 911 results for: TRO

...Using a spectrophotometer is among the faster methods of quantification. Many spectrophotometers have an... you have multiple contaminating bands or a few strong ones, consider increasing the number of washes ... period of two weeks. After this time, quality control assays showed that the majority of antibodies are...references More resources on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant Antibodies...Protein aggregation - Mechanisms, detection, and control. Int J Pharm. 2018 Oct 25;550(1-2):251-268. doi.../j.cocis.2014.10.002. Randolph TW, Schiltz E, Sederstrom D, Steinmann D, Mozziconacci O, Schöneich C, ...

... to introduce multiple genetic modifications in the same strain. MazF expression is under control of the... a GFP cassette, and introduced site-directed mutations, all without introducing unwanted selection markers...delivery vector can be integrated into the genome to introduce the genetic modification(s) contained within it...EcoRI and Bsp6I in Escherichia coli in 1995 when introduction of restriction-modification systems onto plasmids... able to monitor lacZ activity over 48 hours in vitro without antibiotic selection. In a subcutaneous ...

...derived from the control trace file. On the plot, the green (edited sample) and orange (control sample) lines...cells, genomic DNA from both edited and unedited (control) populations is PCR-amplified and Sanger sequenced...determined by comparing the edited trace to the control trace. In the ICE algorithm, potential editing ...summary table. You can also use your browser’s Control+F or Command+F functionality to search for a particular...disagreement between the non-edited wild type (control) and the edited sample in the region around the...difference. Traces The Traces tab shows the edited and control, non-edited Sanger traces in the region around ...

...for this task. However, most lesioning methods destroy areas larger than intended and may unintentionally...to the neuronal networks involved in directly controlling muscle activity and coordinating movement, several...optogenetics to support these existing findings by controlling neurons in the mPFC with light. Activation of...inhibited by fear/anxiety. On the other hand, the control group preferred staying in the dark compartment...time the animal spends in the passive mode, the stronger is the depressive component of the animal behavior... such genetic study focuses on brain derived neurotrophic factor (BDNF). BDNF is a small protein involved...the same paper, the authors used lentivirus to introduce an shRNA to downregulate expression of miR124a...

...unproductive conversations with internet trolls (urban dictionary for trolling: The art of deliberately, cleverly...to write our AMA submission to avoid rousing the trolls. 3. The AMA process was simple and well organized...while it can be anonymous, I can always block the trolls. It seemed like I would really have to open myself...moderators were on top of any comments that were at all trollish. Kudos to the moderators for keeping the quality...discourse. They even advised me on how to alter my intro to reduce the number of potentially hostile posters... the term “implicit bias” which is apparently a troll trigger phrase these days). Redditors ask serious...

...approach complete with freely available positive controls. Routinely test your cell line for mycoplasma ...freezing virus in a dry ice/ethanol bath or liquid nitrogen prior to storing. Know your target cells Labs ...These binding events can be impaired by the electrostatic repulsion of the negatively charged cell with..., A., Garnier, A. “Downstream processing of oncoretroviral and lentiviral gene therapy vectors.” Biotechnology...conditions on the production of lentiviral and retroviral vectors.” Human Gene Therapy Clinical Development...Cronin, J., Zhang, X.Y., Reiser, J. “Altering the tropism of lentiviral vectors through pseudotyping.” Current...Cleghorn G., West M.J., Wei MQ. “The significance of controlled conditions in lentiviral vector titration and...

...Optogenetics Guide γ-Retrovirus Guide Protocols Agarose Gel Electrophoresis Bacterial Transformation... direct shipments weren’t able to clear import control. Partnering with a distributor allowed us to share... How to Design Primers for PCR Agarose Gel Electrophoresis Inoculating Liquid Bacterial Cultures Restriction...Restriction Digest Analysis We also introduced guides — practical primers to help users get started ...we launched the Addgene YouTube channel to add a strong visual component to our educational resources. ... systems to allow for easier access to export-controlled items by streamlining permissions and paperwork...

...different scientific fields, and knowledge of how to troubleshoot problems at the bench. Specifically, roles in...loads of questions, and I also had to do some troubleshooting as advised by the technical support person....specificity of this antibody?” You will also help troubleshoot products, give general experimental guidance...the world easier. You should also demonstrate a strong willingness to help others with their problems,... initial contact, but is less suitable for troubleshooting or issuing replacement products. In order to...careers are open to experienced professionals with strong interest in the technical aspects of science and...work includes reviewing customer data sets, troubleshooting protocols, analyzing information, and solving...

... channelrhodopsin in the retrograde serotype, and a dTomato under the control of the E6 regulatory element...pAAV-CAG-emiRFP670 AAV1 Control Kiryl Piatkevich New viral tool pAAV-S5E6-dTom-nlsdTom AAV1 Control Jordane Dimidschstein...Wilson New serotype AAVrg = AAV retrograde (also called AAV2-retro) Looking for more viral vectors? ...

...field of view. Tetbow can be introduced in vivo either by electroporation of plasmids, or using AAV vectors...type of cell labeling technique involves the introduction of a transgene construct containing fluorescent...cells, the expression level of these XFP is not strong enough for all applications. To resolve this, similar...use in Drosophila melanogaster and is under the control of Flp recombinase. The XFPs in Flybow are membrane-tethered...entire organism or a specific tissue. XFPs are introduced using a plasmid construct called Zebrabow-GateDest...Additional resources on the Addgene blog Read an introduction to live cell imaging Learn more about the origin...