We narrowed to 888 results for: TRI

...This post was contributed by guest bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss...relative nuclease efficiencies as well as to more stringent targeting rules that result in a decrease in available...

...This post was contributed by Greg Dingle, a software engineer with the Chan Zuckerberg Initiative. We...McMaster University in Canada. He's happy to be contributing again to science.   Acknowledgements Chan Zuckerberg...

...standard curves, and normal controls must be run in triplicate.  The nice thing about qPCR is that since it ... of reactions, it does not need to be run in triplicates. ddPCR is an extremely accurate application, ...

...under which the plasmid is distributed.  All Addgene plasmids are distributed under the terms of the UBMTA...

...are treated with a drug of interest and gRNA distribution is analyzed in the drug-resistant population...6 gRNAs per target gene, and maintaining the distribution of each gRNA within the population is key. Loss...

...This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery ...along the illumination path. The shadows appear as stripes in the acquired image (5).  A way to get around...

...multicistronic vectors work? Scientists have "borrowed" some tricks discovered in positive single-stranded RNA viruses...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...

...This post was contributed by Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how... short as 100ms (EBFP) or as long as 1 hour (mAmetrine1.2). However, for most FPs it is a few seconds ...

...noticing unusual cases of acute hepatitis in the pediatric population. Once ~400 cases had amassed in places...removal of certain adenoviral genes that apparently contribute to virulence (Tollefson et al., 1996) – cell ...

...’ve got you covered in this blog, with tips and tricks for harnessing the homology-directed repair pathway...are overly confluent or don’t have sufficient nutrients in their media, they will not undergo DNA replication...

...This post was contributed by Laura Lee, a graduate student at Stanford University. Arabidopsis is a fantastic... it pretty much is, but there are some tips and tricks you can use to obtain optimum transformation efficiency...

...conditions commonly encountered in human gene therapy trials. Human Gene Therapy Methods, 26(2), 71–76. https...Qu, G. (2005). Identification of factors that contribute to recombinant AAV2 particle aggregation and ...

...This post was contributed by guest blogger Tessa Cressey. The highly pathogenic Ebola virus belongs to...Vectors Check out Our Scientist's Guide to Ebola Contribute to the Addgene Blog Resources on Addgene.org...

...functions differently. Since Cpf1 loci are widely distributed across bacterial species, Zetsche et al. hypothesized...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...

... This post was contributed by Jo Miller, founder and principal of J. Miller & Associates, Inc., a national...professionals is learning to succeed in grants through trial and error, through winning and losing funding, and...

...them! Note: A. Max Juchheim, Marcy Patrick, and Meghan Rego contributed to the writing of this article. ...

...an elderly family member, want to train for a triathlon, finally buy that charming fixer-upper house you... every school performance, chaperone every field trip or cheer at every soccer game. Tip: take turns going...

...the host organism being unable to synthesize a nutrient required for growth. Before delivery of the vector...cells are grown in media that supplies the key nutrient. The vector provides the missing synthesis machinery...

...than any other one. Luckily, you can do a lot to contribute to the relationship dynamic and set yourself ...notes on general protocol steps and other tips and tricks your mentor shares. Additionally, go ahead and ...

... libraries are typically DNA preps, but we do distribute several of our most popular CRISPR pooled libraries...cells at a concentrated seeding density while centrifuging. Spinfection transduction efficiency can vary...