We narrowed to 922 results for: POR

1. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   ...the deletion. REPLACR-mutagenesis successfully incorporated a 60-nucleotide insertion; beyond this insertion...

2. Plasmids 101: Blue-white Screening

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   ...they should be. Don't rush the process: It is important to give your plates enough time for any intact...

3. Antibodies 101: Conjugation

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   ...find yourself doing your own conjugation, it is important to keep in mind that you will need to confirm ...

4. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ...research. But be aware that the fluorescent proteins reported in publications are only characterized under a...

5. CRISPRainbow and Genome Visualization

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   ...similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA...

6. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   ...understanding all of the players becomes ever more important to ensure that you choose the most appropriate...

7. RNA Extraction Without A Kit

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   ...extractions- if you need really clean RNA, it’s important to make sure that when extracting, to only take...

8. Antibodies 101: Secondary Antibodies

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   ...subclasses, such as mouse IgG2 or IgG4. This can be important for some applications. Assay use While secondary...

9. What's New in CRISPR - November 2021

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   ...making it well suited for AAV packaging. Most importantly, this CasMINI system was shown to be effective...

10. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    ...into a hole in their cage. Since dopamine is an important neurotransmitter that promotes reward-motivated...

11. Summer SciComm Series: Cognitive Load

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    ... too little)?  The answer is: it depends! The important point here is to pay attention to exactly what...

12. Developing Lab Management Software for Biology

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    ...being treated in the lab, because of this, it is important to write loosely coupled, modular code. Use tried-and-true...

13. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    ... for West-Nile-virus-induced cell death." Cell reports 12.4 (2015): 673-683. PubMed PMID: 26190106. PubMed...

14. New Tool for Lineage Tracing: The ClonTracer Library

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    ...leukemia. Bhang says the work highlighted the importance of developing more potent ABL1 allosteric inhibitors...

15. Plasmids 101: Golden Gate Cloning

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    ...like Gateway cloning or Gibson assembly. Another important consideration is the design of flanking overhangs...

16. Plasmids 101: Cre-lox

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    ... Plasmids 101 series, we examined a number of important plasmid elements – promoters, origins of replication...

17. Plasmids 101: The Wide World of Natural Plasmids

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    ...plasmids — more on that in a minute! Fun fact! Electroporation is one popular method scientists use to promote...

18. Plasmids 101: Modular Cloning

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    ...phenotype, translational output, etc., which can support a larger cellular engineering goal, several of ...

19. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ...while also capturing information about spatial and temporal organization. The design use to detect PKA activity...

20. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ...basic research and other ongoing clinical trials support the utility of AAV as a broadly applicable gene...

21. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ... but instead selectively sequence the variable portion of the plasmid in the pool - ex. the gRNA in a ...

22. Adenoviral Vector Production and Troubleshooting

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    ... quickly due to immune response against AdV. Importantly, recombination-competent adenovirus (RCA) can...

23. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...Hybridization (WISH) WISH is a technique that detects the temporal and spatial localization of mRNA transcripts in...

24. Tips for Titering Your Lentiviral Preps

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    ...the sample regardless of whether or not it is incorporated into a viral particle. Consequently, titers ...

25. Experimenting in an Open Source lab: from CRISPR to Cats

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    ... funded by someone else (KittyBiome has been incorporated as a non-profit entity and so far most of their...

26. Common Injection Routes in Mice

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    ...muscle groups (usually the thigh), though it is important to avoid injection into the sciatic nerve. Mice...

27. Viral Vectors 101: Producing Your rAAV

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    ...different titers are measured. A TCID50 measurement reports an infectious titer, meaning the concentration ...

28. Antibodies 101: Flow Compensation

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    ...change your compensation values during the analysis portion of your experiment. So, as long as you are able...

29. Of Myc and Men

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    ... can find it in likely mean it will remain an important tool for the foreseeable future. These newer findings...

30. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...improvement in off-target:on-target editing ratio. Importantly, the observed off-target loci displayed a broad...