We narrowed to 912 results for: ire;

...probably never use the Illumina GAIIx we recently acquired  for sequencing again - it’s too inefficient and...

...however, is a “helper-free” system, in which the required adenoviral helper genes are supplied on two different...

...hundreds to thousands of different pegRNAs for each desired edit, it simply becomes impossible (or at least...

... blog posts are also frequently read: Homology Directed Repair has been viewed 219,289 times, while Validating...

...gone up to a poster where the presenter spends the entire time talking to the poster, as opposed to engaging...

...-CRISPRi is stably expressed with no selection required and titratable knockdown allows for the study ...

...accurately read (Bell, 2018.) Even though it does not require a standard curve or a normal control, it can take...

...resolution. Creative negotiation can also decrease or entirely eliminate the feeling of conflict many of us associate...

...pre-set timers for incubation steps, and save the entire process so you’ll have a record of your work. This...

...alternatives Depending on your sequencing result and desired analysis, BLAST may not always be your optimal ...

...software, don’t give up! Rather than plotting the entire data set, plot only the linear portion. This allows...

...racial bias from work being done today, is a nearly entirely separate effort from acknowledging its existence...

...And, many wanted to learn more, even those not directly involved in the project.  Once we gauged interest...

... to help the DNA enter the cells. Some samples require a reduced antibiotic concentration or need to be...

...cytometry, but rarely used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification...

...that charming fixer-upper house you have always admired or volunteer to lead a charitable cause.  While...

...tools that best fit their specific experimental requirements. Best-in-class collection at Addgene The GTA...

.... The supporting data and community engagement required to do this drove Addgene to develop new systems...

... addition to the neuronal networks involved in directly controlling muscle activity and coordinating movement...

...the production of a cell surface glycan that’s required for ricin uptake. The ricin screen did fail to...

...lines of showing that the checkout takes the user directly to the shipping step, and no longer takes the ...

...convert the citation style to whatever the journal requires. Using the (Last name, Year) format also has the...

... can also lend their power to other cells when required, through mitochondrial transfer. In the two decades...

...Your mental health and success in science will directly depend on your science mentor. One scientist who...

...and ideas toward solving one problem. It often requires thinking about a problem in a new way through ...

...gene editing process. See below for details. The entire process from start to finish took about 1 month...

...of interest or using non-natural amino acids to directly incorporate a fluorophore in your protein or to...

...oligos would even accept the Output_Short file directly. We have used CustomArray, Inc for this purpose...

...Sharps container Biohazard waste container Fire blanket Fire extinguisher Guidelines BSL-1 Guidelines Before...eyewash station, safety shower, fire blanket, and extinguisher are also required to be present in the room....each level, as each level has different safety requirements. BSL-1 is designated for those working with ...times when you may be working with a protocol that requires shaking or mixing, which may produce aerosols ... PPE protocols . Working in a BSL-2 laboratory requires laboratory glasses in addition to coat and gloves...room and on the door. BSL-2 centrifugation steps require an aerosol-tight lid and rotors should be loaded...in a biosafety cabinet. BSL-2 safety protocols require bloodborne pathogens training. It is strongly recommended...

...stringent buffers and a sonication step may be required for hard to extract proteins such as those in ...standard curve. Determine the volume of sample required to load an equivalent amount of total protein ...continue running the gel until you have obtained the desired separation. Gently open the gel with the spatula...Device. Close the lid of the device. Select the desired method and make sure the parameters are correct... incubation Dilute the primary antibody to the desired concentration in blocking buffer. Pro-Tip The optimal...peroxidase-conjugated secondary antibody to the desired concentration in blocking buffer. Pro-Tip The optimal...