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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 910 results for: ire;

Showing: 841 - 870 of 910 results

  5. Transferable Skills: Negotiation

    Type
    Blog Post
    ...resolution. Creative negotiation can also decrease or entirely eliminate the feeling of conflict many of us associate...

  14. Plasmids 101: Protein tags

    Type
    Blog Post
    ...cytometry, but rarely used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification...

  27. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...Sharps container Biohazard waste container Fire blanket Fire extinguisher Guidelines BSL-1 Guidelines Before...eyewash station, safety shower, fire blanket, and extinguisher are also required to be present in the room....each level, as each level has different safety requirements. BSL-1 is designated for those working with ...times when you may be working with a protocol that requires shaking or mixing, which may produce aerosols ... PPE protocols . Working in a BSL-2 laboratory requires laboratory glasses in addition to coat and gloves...room and on the door. BSL-2 centrifugation steps require an aerosol-tight lid and rotors should be loaded...in a biosafety cabinet. BSL-2 safety protocols require bloodborne pathogens training. It is strongly recommended...

  28. Western Blot

    Type
    Protocol
    ...stringent buffers and a sonication step may be required for hard to extract proteins such as those in ...standard curve. Determine the volume of sample required to load an equivalent amount of total protein ...continue running the gel until you have obtained the desired separation. Gently open the gel with the spatula...Device. Close the lid of the device. Select the desired method and make sure the parameters are correct... incubation Dilute the primary antibody to the desired concentration in blocking buffer. Pro-Tip The optimal...peroxidase-conjugated secondary antibody to the desired concentration in blocking buffer. Pro-Tip The optimal...

  29. Kit Free RNA Extraction

    Type
    Protocol
    ... as bone, or bacteria/yeast/plant samples will require additional steps to effectively lyse open the cells...wash and add RNase-free water or TE as soon as the entire tube is dried but while the white pellet is still...Pro-Tip To avoid multiple freeze-thaw cycles of your entire RNA sample, consider making smaller aliquots of...

  30. CRISPR Library Amplification

    Type
    Protocol
    ...possible but note that individual libraries may require modifications dictated by the originating laboratory... can be scaled according to the actual number. Required Quality Control (QC) Pooled libraries can be challenging...a smaller plasmid containing only the elements required for bacterial propagation (origin of replication...100,000 plasmids. Colonies may appear small and require extra incubation time in order to be enumerated...colonies but as long as one can ensure more than the required colonies are present. If colonies are not present...can dramatically plateau and sometimes fall off entirely if the column or reagents are significantly overloaded...
Showing: 841 - 870 of 910 results