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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 712 results for: BASE

Showing: 561 - 580 of 712 results

  2. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...beyond generating frameshift mutations or single base pair edits, these enzymes are worth taking a look...Cas3 DNA digestion can proceed up to multiple kilobases, but only in one direction. Cas3 is thus not helpful...

  8. Caltech Systemic Capsids

    Type
    Collection
    ...quality control, including AAV titering by probe-based droplet digital PCR, in vitro and (when possible...

  10. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ...used to restrict expression to a single cell type based on the overlap in cell types both promoters expressed...Institute of Molecular and Clinical Ophthalmology Basel recently took a different approach to enhance specificity...targeting the same cell class in humans (R=0.67).  A database of the microscopy images of all AAVs that reproducibly...

  11. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite expression system that can regulate gene... is curated and added to other databases including wormbase and flybase. microPublication is run and maintained...pages and curated databases for scientists including Wormbook, Wormatlas and Wormbase. And scientists have...institute developed a 3’UTR database specifically for C. elegans. This database provides detailed information...coding mRNAs, and annotations extracted from other databases (Mangone et al., 2008).  OpenWorm OpenWorm is ...published there as well.   Worm Development Dynamics Database (WDDD) The WDDD aims to acquire quantitative data...Koji, et al. "WDDD: worm developmental dynamics database." Nucleic acids research 41.D1 (2012): D732-D737...

  14. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...you can extract practically every single reliable base pair from your results. This option is not as fast...sequence databases from the dropdown menu. Typically, we use the default nucleotide database “Nucleotide...plasmids with us or other entries in NCBI’s numerous databases. As our repository has grown over the years (we... explanation of the different BLAST programs, databases, and elements of the BLAST search pages. At Addgene...known reference sequence or to a BLAST sequence database. If you know the expected nucleotide sequence,...identity between your sequence and the reference/database sequence is ≥ 95%. [Our QC process would be trouble-free...blastx checks all six possible frames against the database. Another benefit is that a frameshift mutation...

  15. Deisseroth INTRSECT Collection

    Type
    Collection
    ...virus (AAV)-borne payloads to be expressed in cells based on a doubly-specified combination of genetic and...

  17. Rabies and Neuronal Tracing

    Type
    Blog Post
    ... target cell surface receptors (Callaway 2008). Based on this, cell type specificity of the starter cell...

  18. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...to help you learn more about fly biology and fly-based experimental systems. Some resources will direct...book for newbies on how to work with flies. Flybase.org is absolutely essential. You can look up information...many fly stocks that are available to everyone. Flybase lists what stocks are available at Bloomington,...
Showing: 561 - 580 of 712 results