We narrowed to 644 results for: CAR
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TypeBlog Post...below. The main requirement for IVA cloning is carefully designed primers. The majority of mistakes are...
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AAVs in Retinal Gene Therapy
TypeBlog Post...Its main limitation is the small size of its DNA cargo - 4.8 kb at most - which means that a gene larger... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post...science and society." Science 376, (2022): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog Post... the outcome of your IF experiment and deserve careful thought and attention. * There are other methods... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog Post...Mozziconacci O, Schöneich C, Freund E, Ricci MS, Carpenter JF, Lengsfeld CS. Do not drop: mechanical shock... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...experiments must be set up, conducted and analysed with care to get reliable information. Although two-hybrid... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post...line. When expressing a viral construct transiently care needs to be taken to ensure that the proper cell... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...Ampicillin VWR: #7177-48-2. Use at 100 μg/mL. Carbenicillin VWR: #80030-956. Use at 100 μg/mL. C.2 Annealing...agar plates containing 100 μg/mL ampicillin or carbenicillin (an ampicillin analog). Due to the long terminal... ligation into LB + 100 μg/mL ampicillin or carbenicillin. Day 2: 2. Spin down the cultures and use a ... to 55°C. Add 1mL of 100mg/mL ampicillin or carbenicillin to obtain a final concentration of 100 μg/mL...distribution of any literature by Addgene is not meant to carry with it, and does not grant any license or rights...distribution of materials by Addgene is not meant to carry with it, and does not grant any license, express... -
Protocol - How to Inoculate a Bacterial Culture
TypeProtocol...Bacterial Culture Background Information Plasmids can carry one or more antibiotic resistance genes, which confer...resistance to a specific antibiotic to the bacteria carrying them. The presence of an antibiotic resistance...isolate individual (clonal) colonies of bacteria carrying a specific plasmid. However, a liquid culture ...Concentration Ampicillin 100 µg/mL Bleocin 5 µg/mL Carbenicillin 100 µg/mL Chloramphenicol 25 µg/mL Coumermycin... -
Hot Plasmids - August 2020
TypeBlog Post...markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within... -
An Inside Look into Addgene's Dev Team
TypeBlog Post...Figure 1: Addgene’s Agile Approach Sharing is caring There are many moving parts to our software, which... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...followed bylanding site recognition and integration of cargo via Cas9-directed integrases. Use PASTE can... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog Post...and muscle contractions (think skeletal muscle, cardiac muscle, and your airways and blood vessels). Spaghetti... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post...may exhibit slow head growth, develop seizures, cardio-respiratory dysfunction, distinctive hand movements... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post...it doesn’t eliminate the need to deliver larger cargo such as base editors and prime editors. While using... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog Post...continue to develop improved, reliable tools to help carry out that research. Organizations like the Allen ... -
QC Sequencing Technologies at Addgene
TypeBlog Post...significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary References and Resources... -
Antibodies 101: Reading a Flow Plot
TypeBlog Post...you realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted by the amount... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...up with new colors (Fig. 3), such as Radish and Carrot, and further means to increase aptamer stability... -
How to Write a Scientific Review Article
TypeBlog Post...the themes in the field and to learn what people cared about. I also used reviews to get a list of research...