We narrowed to 641 results for: CAR

1. AAVs in Retinal Gene Therapy

   Type

   Blog Post

   ...Its main limitation is the small size of its DNA cargo - 4.8 kb at most - which means that a gene larger...

2. Uncovering Ancestral Bias in CRISPR Research

   Type

   Blog Post

   ...science and society." Science 376, (2022): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter...

3. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

   Type

   Blog Post

   ... the outcome of your IF experiment and deserve careful thought and attention.   * There are other methods...

4. Tips for Screening with Yeast Two Hybrid Systems

   Type

   Blog Post

   ...experiments must be set up, conducted and analysed with care to get reliable information. Although two-hybrid...

5. Your Lentiviral Plasmid FAQs Answered

   Type

   Blog Post

   ...line. When expressing a viral construct transiently care needs to be taken to ensure that the proper cell...

6. Protocol - How to Inoculate a Bacterial Culture

   Type

   Protocol

   ...Bacterial Culture Background Information Plasmids can carry one or more antibiotic resistance genes, which confer...resistance to a specific antibiotic to the bacteria carrying them. The presence of an antibiotic resistance...isolate individual (clonal) colonies of bacteria carrying a specific plasmid. However, a liquid culture ...Concentration Ampicillin 100 µg/mL Bleocin 5 µg/mL Carbenicillin 100 µg/mL Chloramphenicol 25 µg/mL Coumermycin...

7. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

   Type

   Blog Post

   ...continue to develop improved, reliable tools to help carry out that research. Organizations like the Allen ...

8. QC Sequencing Technologies at Addgene

   Type

   Blog Post

   ...significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary References and Resources...

9. Antibodies 101: Reading a Flow Plot

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   Blog Post

   ...you realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted by the amount...

10. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    ...followed bylanding site recognition and integration of cargo via Cas9-directed integrases.   Use PASTE can...

11. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    ...it doesn’t eliminate the need to deliver larger cargo such as base editors and prime editors. While using...

12. Hot Plasmids - August 2020

    Type

    Blog Post

    ...markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within...

13. An Inside Look into Addgene's Dev Team

    Type

    Blog Post

    ...Figure 1: Addgene’s Agile Approach Sharing is caring There are many moving parts to our software, which...

14. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    ...up with new colors (Fig. 3), such as Radish and Carrot, and further means to increase aptamer stability...

15. How to Write a Scientific Review Article

    Type

    Blog Post

    ...the themes in the field and to learn what people cared about. I also used reviews to get a list of research...

16. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    ... clones. The entry clone and destination vector carry different antibiotic resistance markers (indicated...

17. Mesothelioma - Causes, Symptoms, and Treatment

    Type

    Blog Post

    ... The best option in such instances is palliative care, which helps patients manage the pain and discomfort...

18. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    ...and muscle contractions (think skeletal muscle, cardiac muscle, and your airways and blood vessels). Spaghetti...

19. Tips for CRISPR Gene Editing in Mice

    Type

    Blog Post

    ...Wang, Haoyi, et al. "One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated...

20. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type

    Blog Post

    ...may exhibit slow head growth, develop seizures, cardio-respiratory dysfunction, distinctive hand movements...