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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 768 results for: Dos

Showing: 561 - 580 of 768 results

  2. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by introducing... the culture, so bacteria must be grown in a turbidostat. Only mutate particular types of nucleotides...

  6. Components of CRISPR/Cas9

    Type
    Blog Post
    ...about the types of experiments you're planning on doing and read the eBook or browse our CRISPR resources...

  8. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...) antigens. Unfortunately, antibodies frequently do not work as assumed (Ayoubi et al., 2023). Additionally...may not be available to every scientist looking to do a flow cytometry experiment. But validation is critical...

  9. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...domains—including the CTD and the entire REC lobe—do not structurally resemble any other known proteins...check for sequence matches; a perfect guide RNA will do you no good if there is no adjacent PAM to anchor...

  12. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...and are slightly different in sequence. Both LTRs do share common repeat regions in addition to their ...virion assembly and particle release from late endosomes when AAV is trafficked. Rep and cap mediate AAV...

  13. All in a Twist: dsRNA

    Type
    Blog Post
    ...produced dsRNA rarely reach this threshold and thus do not typically activate an inflammatory response. ...
Showing: 561 - 580 of 768 results