We narrowed to 750 results for: Dos

...Guide or find CRISPR/Cas plasmids for your research. Do you have a cool idea or application that involves...

...conventional FPs for super-resolution imaging, they still do not emit the large number of photons achievable with...use in yeast, as parts of biosenors, and much more. Do you have a favorite use for PA-FPs? Let us know in...

...sites — similar to restriction enzyme cloning. To do this, your DNA fragment must first be amplified with...

...the histogram plots look a bit different than they do for yes-no readouts.      Figure 4: A histogram...

... disillusioned with the gulf between what I was doing in the lab and the interactions I was having outside...

...monoclonal antibody. But what does that mean, and how do monoclonal antibodies differ from other types of ...

...You can find more information about us and what we do in our Frequently Asked Questions. We also invite...

...spatio-temporal dynamics of apoptosis in living animals. To do this they modified a different self-assembling split...

...chromosomal loci in human cells (Ma et al., 2015). To do so, they fused a different fluorescent protein to...

...could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by introducing...

... their low leakiness. Inducibility level How much do you want to induce transcription of your target gene...

...students are asking. Teachers are also asking, “How do I engage my students in real-world lab activities...

...majority of restriction enzymes commonly used in the lab do not have recognition sites that could overlap with...

...about the types of experiments you're planning on doing and read the eBook or browse our CRISPR resources...

...) antigens. Unfortunately, antibodies frequently do not work as assumed (Ayoubi et al., 2023). Additionally...may not be available to every scientist looking to do a flow cytometry experiment. But validation is critical...

...domains—including the CTD and the entire REC lobe—do not structurally resemble any other known proteins...check for sequence matches; a perfect guide RNA will do you no good if there is no adjacent PAM to anchor...

...resources on the Addgene blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? CRISPR 101: ...

...and are slightly different in sequence. Both LTRs do share common repeat regions in addition to their ...

...produced dsRNA rarely reach this threshold and thus do not typically activate an inflammatory response. ...

...broader community, and preprints are a great way to do that. We are happy to receive deposits of plasmids...