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A look at the history of the CRISPR/Cas system. Discovered in bacteria, this system has been harnessed for genome engineering.

Learn about different molecular cloning techniques, focusing on seven common cloning methods.

Photoactivatable fluorescent proteins can be switched on (and sometimes off) enabling a variety of techniques and applications.

If your flow plot has a smear instead of distinct populations, you can still intepret the data! In this post, we discuss how to interpret plots of all kinds.

Hear from Crowdfight members to see how their platform got started during COVID-19 to help connect scientific research and learn how they're expanding!

Learn about new plasmids at Addgene including CRISPR, auxin inducible degradation, MerMAID channelrhodopsins, and genome looping.

CRISPR-Sirius is a new tool to visualize genomic loci using dCas9. It's brighter than its predecessor CRISPRainbow and unlike fluorescent in situ hybridization, can be used in live cells.

Learn how you can use the Biobits plasmids and freeze-dried cell extracts to teach students about molecular biology.

Some promoters are only active under specific circumstances. Here's a guide to inducible promoters, which are regulated by chemicals, light, and/or temperature.

Find out how you can get involved with BioBuilder - a nonprofit bringing hands-on synthetic biology experiences to the classroom.

Two techniques developed by Addgene depositors have made it possible to track mRNA translation at the single molecule level using fluorescent proteins.

Addgene Scientist Eric Perkins gives insights into the stem cell field he gained from the 10 years of iPSCs symposium

A quick guide on the main components of CRISPR: the Cas9 endonuclease and the guide RNA.

Using synthetic biology principles has simplified and improved plasmids for optogenetic control of gene expression in E. coli.

If you can't find a validated antibody for your flow experiment, this blog post can help you validate your antibodies yourself.

Curious about how, exactly, Cas9 does what it does? Dive into the structural biology of Cas9 for an answer!

How do we know if a therapeutic antibody is a promising clinical prospect? In vivo and in silico methods can help us decide!

A review of the CasPEDIA resource and its features.

Description of the components of the 4 most common viruses - AAV, adenovirus, lentivirus, and gamma-retrovirus.

A review of the cellular functions and consequences of dsRNA and RNA:DNA hybrids.